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E-grāmata: Analyzing Microbes: Manual of Molecular Biology Techniques

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  • Formāts: PDF+DRM
  • Sērija : Springer Protocols Handbooks
  • Izdošanas datums: 03-Feb-2013
  • Izdevniecība: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Valoda: eng
  • ISBN-13: 9783642344107
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Analyzing Microbes: Manual of Molecular Biology TechniquesPalielināt
  • Formāts: PDF+DRM
  • Sērija : Springer Protocols Handbooks
  • Izdošanas datums: 03-Feb-2013
  • Izdevniecība: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
  • Valoda: eng
  • ISBN-13: 9783642344107
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This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.



This Springer Protocols manual offers authoritative practical guidance on applying key techniques in molecular biology to microbiological research, focusing on experimental protocols with stepwise instructions that make for easy laboratory reproduction.

Recenzijas

From the reviews:

The Analyzing Microbes­Manual of Molecular Biology Techniques is indeed a practical guide to the application of various molecular biology techniques in general microbiological research, with special emphasis in/on biotechnology and environmental microbiology. All chapters are written in a concise step-by-step algorithm and are easy to follow during reading or bench working. In my opinion, the aim of this book to produce a self-contained laboratory manual which will be useful to both experienced practitioners and beginners in the field, is fully accomplished. (Ioan I. Ardelean, Romanian Journal of Biochemistry, Vol. 50 (1), 2013)

1 Microbial DNA Extraction, Purification, and Quantitation
1(16)
Sukumar Mesapogu
Chandra Mouleswararao Jillepalli
Dilip K. Arora
2 Fluorescent-Based Detection, Quantitation, and Expression of Viral Gene by qRT-PCR
17(14)
Shelly Praveen
Vikas Koundal
3 Restriction Enzymes and Their Role in Microbiology
31(6)
Sukumar Mesapogu
Chandra Mouleswararao Jillepalli
Dilip K. Arora
4 Genetic Fingerprinting Techniques for Molecular Characterisation of Microbes
37(36)
Annette Reineke
K. Uma Devi
5 Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis: Methods and Principles
73(20)
Sukumar Mesapogu
Chandra Mouleswararao Jillepalli
Dilip K. Arora
6 Molecular Identification of Microbes: I. Macrophomina phaseolina
93(6)
Bandamaravuri Kishore Babu
T. Kiran Babu
Rajan Sharma
7 Molecular Identification of Microbes: II. Bacillus
99(6)
Anil Kumar Saxena
8 Molecular Identification of Microbes: III. Pseudomonas
105(8)
Bhim Pratap Singh
Ratul Saikia
9 Molecular Identification of Microbes: IV. Vibrio
113(10)
Hirak Ranjan Dash
Neelam Mangwani
Surajit Das
10 Molecular Identification of Microbes: V. methylotrophs
123(12)
Kamlesh K. Meena
Manish Kumar
D.P. Singh
11 Preservation and Maintenance of Microbial Cultures
135(18)
Sudheer Kumar
Prem L. Kashyap
Ruchi Singh
Alok K. Srivastava
12 Microbes from Extreme Environment: Molecular Identification Procedures
153(16)
Surajit Das
13 ELISA-Based Identification and Detection of Microbes
169(18)
Jyoti Verma
Sangeeta Saxena
Sunil G. Babu
14 Analysis of Microbial Diversity and Construction of Metagenomic Library
187(22)
Thangamani Rajesh
Jeyaprakash Rajendhran
Paramasamy Gunasekaran
15 Bioinformatics Tools for Interpretation of Data Used in Molecular Identification
209(36)
Suchi Smita
Krishna P. Singh
Bashir A. Akhoon
Shishir K. Gupta
Shailendra K. Gupta
16 Molecular Phylogenetics of Microbes
245(16)
Surajit Das
Hirak Ranjan Dash
17 Microarray Technology: Basic Concept, Protocols, and Applications
261(20)
P.P. Dubey
Dinesh Kumar
18 Microarray Analysis of Different Functional Genes of Microorganisms
281(10)
Hirak Ranjan Dash
Surajit Das
19 DNA Cloning and Sequencing
291(12)
Bandamaravuri Kishore Babu
Anu Sharma
Hari Kishan Sudini
20 Biological Sequence Analysis: Algorithms and Statistical Methods
303(32)
Suchi Smita
Krishna P. Singh
Bashir A. Akhoon
Shailendra K. Gupta
Appendix 1 Biosafety, GLP, and Biosecurity 335(4)
Appendix 2 Address for Instruments and Chemicals Suppliers 339(6)
Index 345
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