Animal Models of Disease, Part D, Volume 197 in the Methods in Cell Biology series presents significant advancements in understanding disease mechanisms through animal models. This volume features chapters on timely topics such as characterizing tumor-infiltrating group 1 innate lymphoid cells in PyMT breast tumors and using zebrafish to unravel the molecular mechanisms of Alzheimers disease. The book also covers colitis mouse models and appropriate models for studying diabetes pathophysiology, demonstrating its comprehensive approach to current research.
Additional sections cover evaluating polyglutamine protein aggregation in transgenic Caenorhabditis elegans models of Huntington's disease and studying tumor responses to radioimmunotherapy in ovarian cancer models. It also highlights the antimicrobial regime for gut microbiota depletion in mice and the biodistribution of metallic nanoparticles for lymphoma studies. This book is an essential resource for researchers seeking to explore disease mechanisms and therapeutic approaches through animal models.
Preface
Lorenzo Galluzzi, Fernando Aranda, Aitziber Buque and José Manuel Bravo-San
Pedro
1. Characterization of Mutants of the Fish Pathogen Flavobacterium
psychrophilum.
Mario Garcķa Domķnguez
2. Cannulation of mesenteric lymphatic vessels in a murine model
Zohaib N. Khan
3. Mouse intestine as a useful model for CFTR electrophysiology function
analysis
Maria Chiara Maiuri
4. An immunocompetent mouse model of metastatic triple-negative breast
cancer
Mohamed Jemaa
5. Generation of Humanized Bone for Disease Modeling Using Porcine Adipose
Tissue-Derived Extracellular Matrix Scaffolds and Human Dental Pulp Stem
Cells
Gaskon Ibarretxe, Iratxe Madarieta, Jon Luzuriaga, Nerea Garcķa-Urkia, Jone
Salvador-Moya, Beatriz Pardo-Rodrķguez, Patricia Garcķa Gallastegui, Verónica
Uribe-Etxebarria, Francisco-Javier Fernįndez-San-Argimiro, Igor Irastorza,
Lucķa Jiménez-Rojo, Beatriz Olalde, Fernando Unda and José Ramón Pineda
6. The acute 6-OHDA mouse model
Jose A. Morales-Garcia
7. Methodology and evaluation of the induction of experimental autoimmune
encephalomyelitis, a murine preclinical model of multiple sclerosis
Maria Luisa Nieto
8. Precise Measurement of Motor Neuron Dysfunction in Drosophila ALS Model
via Climbing Assay and Leg Imaging
Yanan Wei, Hongyu Miao, Hadi Najafi and Woo Jae Kim
9. Evaluation of in vivo target cell elimination by antigen-specific
cytotoxic T lymphocytes
Gustavo P. Amarante-Mendes, Abolaji S. Olagunju, Isau H. Noronha and José
Ronnie C. Vasconcelos
10. A protocol for measuring phenotypical facial disease markers in a mouse
model of iatrogenic Cushings syndrome.
Oliver Kepp, Guido Kroemer, Hui Pan, Ai-Ling Tian and Isabelle Martins
11. Swiss Webster mice as a model of alcohol overconsumption
Mohamed Jemaa
12. Orthotopic model of tongue cancer for the study of head and neck squamous
cell carcinoma in mice
Maria Chiara Maiuri, Jonathan Pol, Guido Kroemer, Sarah Adriana Scuderi,
Fatima Domenica Elisa De Palma, Deborah Mannino, Maria Perez-Lanzon, Lionel
Deroche, Marie Valet and Pierre Cordier
13. Experimental model of Unilateral Ureteral Obstruction reproduces key
events of chronic kidney disease: from mice to humans.cide
Lara Valińo, Alberto Lįzaro Fernįndez, Elena Vįzquez Ogando and Marķa įngeles
Gonzįlez Nicolas
14. Animal Models of Obesity
Eugenia Morselli
15. Methods for experimentally increasing circulating acyl coenzyme A binding
protein (ACBP) levels in mice under chronic restraint stress
ISABELLE MARTINS
16. Assessing mitochondrial number and morphology in a C. elegans model of
human tauopathy
Konstantinos Palikaras
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome La Sapienza. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Fernando Aranda holds a BSc in Biology (2006) and Biochemistry (2007) from the University of Navarra. Then, he specialized in different strategies of Cancer Immunotherapy with a MSc in Biomedical Research (2008), and a PhD Degree (2012) from the University of Navarra (Pamplona) Cima University of Navarra. More than 12 years in translational research focus on antitumor immune responses and Cancer Immunotherapy. Author of 64 publications indexed in PubMed in prestigious international journals, with h-index 30 and 4,296 cites (October 2022). He completed the Program of Sara Borrell (ISCIII) -competitive Postdoctoral contract- in the Group of Immune Receptors of the Innate and Adaptive System (IDIBAPS), Barcelona (2016-2018). Co-author of 1 invention patent: Composition based on the fibronectin domain A for the treatment of melanoma - WO/2011/101332. In 2012, Fernando Aranda obtained a Scientific Award, "Profesor Durantez" II Edición, for the best scientific article in Tumor Immunology by Fundación LAIR. Recently, Fernando Aranda awarded a competitive Research Fellow contract Miguel Servet tipo I by Instituto de Salud Carlos III, to continue his independent researcher career (IP) in cancer immunotherapy issues. Specifically, he is involved in Translational Immunotherapy of Peritoneal Carcinomatosis. Currently, Fernando Aranda leads a research group in cooperation with Dr. Pedro Berraondo. Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
