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E-grāmata: Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification

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Volume editor (University of California, Berkeley, U.S.A.), Volume editor (Howard Hughes Medical Institute and School of Medicine, University of California, San Diego, La Jolla, U.S.A.), Volume editor (California Institute of Technology), Editor-in-chief (The Salk Institute, La Jolla, CA, USA)
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 11-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496818
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Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein PurificationPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 11-Oct-2000
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080496818
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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Recenzijas

Praise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE "Incomparably useful." --ANALYTICAL BIOCHEMISTRY "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY "The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY "The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS "If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA "A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY

Contributors to Volume 326 ix Preface xiii Volumes in Series xv Section I. Historical Overview The All Purpose Gene Fusion 3(8) Jon Beckwith Section II. Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli 11(24) Nicholas J. Hand Thomas J. Silhavy Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions 35(12) Colin Manoil Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells 47(26) Raphael H. Valdivia Lalita Ramakrishnan IVET and RIVET: Use of Gene Fusions to Identify Bacterial Virulence Factors Specifically Induced in Host Tissues 73(24) James M. Slauch Andrew Camilli Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin 97(10) Micah J. Worley Fred Heffron Section III. Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast 107(13) Joe Horecka George F. Sprague, Jr. Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae 120(14) Sofie Reda Salama Use of Fusions to Thymidine Kinase 134(12) Christiaan Karreman In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster 146(13) Stephen Small Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme 159(6) Bryan R. Cullen Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters 165(10) Satoshi Inouye Novel Methods for Chemiluminescent Detection of Reporter Enzymes 175(27) Corinne E. M. Olesen Yu-Xin Yan Betty Liu Dina Martin Brian DEon Ray Judware Chris Martin John C. Voyta Irena Bronstein Fusions to Chloramphenicol Acetyltransferase as a Reporter 202(19) Clayton Bullock Cornelia Gorman Fusions to S-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells 221(24) Gregor Zlokarnik Section IV. Functional Tags for Biochemical Purification Purification of Proteins Using Polyhistidine Affinity Tags 245(9) Joshua A. Bornhorst Joseph J. Falke Generating Fusions to Glutathione S-Transferase for Protein Studies 254(17) Donald B. Smith Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins 271(34) Arne Skerra Thomas G. M. Schmidt Streptavidin-Containing Chimeric Proteins: Design and Production 305(7) Takeshi Sano Charles R. Cantor Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification 312(10) Deepali Sachdev John M. Chirgwin Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli 322(18) Edward R. La Vallie Zhijian Lu Elizabeth A. Diblasio-Smith Lisa A. Collins-Racie John M. McCoy Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides 340(22) Peter Vaillancourt Chao-Feng Zheng Danny Q. Hoang Lisa Breister The S Tag Fusion System for Protein Purification 362(14) Ronald T. Raines Mark McCormick Thomas R. Van Oosbree Robert C. Mierendorf Fusions to Self-Splicing Inteins for Protein Purification 376(42) Ming-Qun Xu Henry Paulus Shaorong Chong Fusion Proteins Containing Cellulose-Binding Domains 418(12) Jae-Seon Park Hae-Sun Shin Roy H. Doi Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags 430(10) Millard G. Cull Peter J. Schatz Biotinylation of Proteins in Vivo: A Useful Post-translational Modification for Protein Analysis 440(21) John E. Cronan, Jr. Kelynne E. Reed Section V. Hybrid Proteins for Detection and Production of Antigens and Antibodies Methods for Generating Multivalent and Bispecific Antibody Fragments 461(19) Ian Tomlinson Philipp Holliger Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes 480(26) Francesca Viti Fredrik Nilsson Salvatore Demartis Adrian Huber Dario Neri Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display 506(10) Charles F. Earhart Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes 516(11) Dolph Ellefson Adrianus W. M. van der Velden David Parker Fred Heffron Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells 527(15) Pierre Guermonprez Catherine Fayolle Gouzel Karimova Agnes Ullmann Claude Leclerc Daniel Ladant Use of Anthrax Toxin Fusions to Stimulate Immune Responses 542(9) Christopher C. Zarozinski R. John Collier Michael N. Starnbach Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development 551(20) Peter G. Stockley Robert A. Mastico Author Index 571(30) Subject Index 601
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