This book provides a general introduction of plant tissue culture followed by specific applications of biotechnology in regeneration of rice (Oryza sativa), Maize, Eucalyptus, hot pepper, guava (Psidium guajava L.) stone fruit (Pinus pinea) and compares the features of in vitro grown plants to in vivo plants, Transgenic plants production and application, generating marker-free transgenic plants, genetic engineering and metabolic engineering of plants, molecular farming, abiotic stress tolerance, transgenic in floriculture and ornamental plants, celery, Secondary metabolite production with special reference to sennoside, genetic transformation of potato and biosafety concerns, bioinformatics and its application to crop improvement, Intellectual property rights, biotechnological aspects of secondary metabolite production, application of biotechnology in pharmaceutical sciences and production of recombinant proteins, cyclotides, Hypericum perforatum and Gentiana punctata provide a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. Besides covering basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, gene technology and secondary metabolite production. The book will prove useful to both students and researchers of biotechnology, agriculture, horticulture, forestry as well as for the industry.
1. Tissue Culture: Historical Perspectives and Applications; Section I:
In Vitro Plant Regeneration;
2. High Frequency Plant Regeneration via
Multiple Shoot Induction from Shoot Apex in Indica Rice (Oryza sativa L.)
Varieties;
3. Somatic and Gametic Embryogenesis in Maize: Cell Biology and
Applications;
4. Somatic Embryogenesis in Eucalyptus - An Update to 2009;
5.
Increased Embryo Production by Manipulation of Pretreatment Materials and
Media in Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.).
6.
Biotechnological Advancement in Getting In Vitro Regeneration and Mass
Multiplication in Guava (Psidium guajava L.): A Success Story in Fruit Trees;
7. In Vitro Propagation of Stone Pine (Pinus pinea L.) Molecular and
Physiological Bases;
8. Morphological, Anatomical and Physiological
Characteristics of in vitro Grown Plants in Comparison to ex vitro Grown
Plants; Section II: Transgenic Plants and their Applications;
9. Production
of Transgenic Plants and Their Application in Biotechnology;
10. Practical
Application of Genetic Transformation;
11. Generating Marker-free Transgenic
Plants;
12. Role of recA/Rad51 Gene Family in Homologous Recombination,
Repair and Genetic Engineering of Transgenic Plants;
13. Metabolic
Engineering in Plants;
14. Molecular Farming for Production of Biologically
Important Molecules in Plants;
15. Improving Crop Tolerance to Abiotic
Stresses by Plant Biotechnology;
16. Transgenic Floricultural and Ornamental
Plants;
17. Flux in Morphology and GUS Expression in Somatic
Embryogenic-Derived Populations of Transgenic Celery;
18. The Potato (Solanum
tuberosum L.): An Overview of Genetic Transformation and Related Biosafety
Issues;
19. Clean DNA Transformation of Rice Using Minimal
Gene-Expression-Cassettes to Minimize Biosafety Concerns;
20. Bioinformatics
Application to Crop Improvement;
21. Intellectual Property Rights, Biopiracy
& Patent Revocation; Section III: Secondary Metabolites and medicinal plants;
22. Biotechnological Aspects of Secondary Metabolite Production;
23.
Applications of Plant Biotechnology in Pharmaceutical Sciences: A Critical
Review;
24. Production of Recombinant Proteins in Plants for Pharmaceutical
Use;
25. Intraspecific Variation of Plant Secondary Metabolites;
26.
Optimisation Steps Towards Bioprocess Development for the Production of
Cyclotides;
27. Genetic (In)stability in Hypericum perforatum L.:
Physiological and Induced Sources of Genetic Variation;
28. Biotechnology of
Endangered Medicinal Plants: Spotted Gentian, Gentiana punctata L.;
29.
Direct Shoot Regeneration of Cassia senna for Sustainable Production of
Anthraquinone Glycoside-Sennoside; Index; Author Index.
Ashwani Kumar, Professor, Department of Botany, University of Rajasthan, Jaipur, has earned gold medal in M.Sc, and obtained Ph.D. from Rajasthan University. He also has the distinction of being an Alexander Von Humboldt Fellow (Germany). He was the Honorary Director at the School of Life Sciences, University of Rajasthan. The author's repertoire of published works spreads across 150 research articles in various national and international journals. With an experience of over three decades in his field of research, namely, tissue culture and biochemistry, the author has been at the helm of various major projects carried out by USDA-ICAR, U.G.C., M.N.E.S., C.S.I.R and DBT. He has long association with Professor Dr. Karl-Hermann Neumann and subsequently with Professor Dr. Sven Schubert at Institut Fur Pflanzenernahrung, Justus Liebig Universitat, Giessen, Germany, as visiting Humboldt Fellow and visiting Professor at Toyama Medical and Pharmaceutical University, Toyama, Japan. The author is currently engaged in research on salinity tolerance at molecular level in cereals and crops. Recently he has been awarded the prestigious V. Puri Medal 2008. Sudhir K. Sopory, Head of Plant Molecular Biology Division at the International Centre for Genetic Engineering and Biotechnology, New Delhi, is a well-known Plant Biologist who is recognized for his contributions in research and teaching in the field of Molecular Plant Physiology and Plant Biotechnology. He obtained Ph.D. from the University of Delhi. He is a visiting scientist at Max-Planck-Institute, Kolen, and University of Munich, in Germany, and at the University of Texas, Austin and United States Department of Agriculture, Maryland, USA. Prof. Sopory is one of the pioneers in the country for adapting genetic engineering technologies for the improvement of plant productivity. He has published over 175 papers and over 50 book chapters and co-edited 8 books. Prof. Sopory has received numerous awards and distinctions including Chakravorty Award, Indian Science Congress, 1986 (given by the Vice-President of India); Shanti Swaroop Bhatnagar Award, (CSIR) 1987 (given by the Prime Minister of India); Fellow, Indian Academy of Sciences, Bangalore, 1992; Fellow, Indian National Science Academy, Delhi, 1992; Fellow, National Academy of Sciences, Allahabad, 1994; Birbal Sahni Medal, Indian Botanical Society, 2001; Fellow, National Academy of Agricultural Sciences, 2002; Tenth Godnev Award lecture of the Belarus Academy of Sciences, 2003; Vice-President, Indian National Science Academy, 2004-2006; Birbal Sahni Centenary Gold Medal Award for Life Time Achievement in Plant Sciences, Indian Science Congress Association, 2005 (given by the Prime Minister of India); Fellow: The World Academy of Sciences (Trieste), 2005; Padmashree, 2007, given by the President of India.
