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Bacterial Pathogenesis: Methods and Protocols Second Edition 2023 [Mīkstie vāki]

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  • Formāts: Paperback / softback, 337 pages, height x width: 254x178 mm, 66 Illustrations, color; 4 Illustrations, black and white; XIV, 337 p. 70 illus., 66 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2674
  • Izdošanas datums: 02-Jun-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071632450
  • ISBN-13: 9781071632451
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  • Mīkstie vāki
  • Cena: 145,08 €*
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Bacterial Pathogenesis: Methods and Protocols Second Edition 2023Palielināt
  • Formāts: Paperback / softback, 337 pages, height x width: 254x178 mm, 66 Illustrations, color; 4 Illustrations, black and white; XIV, 337 p. 70 illus., 66 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2674
  • Izdošanas datums: 02-Jun-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071632450
  • ISBN-13: 9781071632451
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071632451.html
Keywords:
This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Bacterial Pathogenesis: Methods and Protocols, Second Edition is a vital resource for researchers in the area of infection biology, as well as but not limited to, those working in the fields of microbiology, immunology, structural biology, molecular biology, genetics, imaging, and computational study.
Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm
Bacteria.- Formation and Analysis of Mono-Species and Polymicrobial Oral
Biofilms in Flow-Cell Models.- Isolation and Purification of Mycobacterial
Extracellular Vesicles (EVs).- Strategies to Isolate Extracellular Vesicles
from Gram - and Gram + Bacteria.- Total Bacterial RNA Isolation and Northern
Blotting Analysis.- Phylogenetic Analysis of Bacterial Pathogen Genomes.-
Determination of Growth Rate and Virulence Plasmid Copy Number
during Yersinia pseudotuberculosis Infection Using Droplet Digital PCR.-
Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to
Streptococcus pyogenes Virulence.- Expression of the Bacterial Enzyme IdeS
Using a GFP Fusion in the Yeast Saccharomyces cerevisiae.- Mass
Spectrometry-Based Methods to Determine the Substrate Specificities and
Kinetics of N-Linked Glycan Hydrolysis by Endo--N-Acetylglucosaminidases.-
Identification of Substrates of Secreted Bacterial Protease by APEX2-Based
Proximity Labeling.- Affinity-Purification Combined with Crosslinking Mass
Spectrometry for Identification and Structural Modeling of Host-Pathogen
Protein-Protein Complexes.- Elucidating the Stoichiometries of Host-Pathogen
Protein Interactions with Mass Photometry.- Analysis of Neutrophil and
Monocyte Inflammation Markers in Response to Gram-Positive Anaerobic Cocci.-
Quantification of Phagocytosis Using Flow Cytometry.- Anti-Bacterial
Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death
Associated with Extracellular Trap Release.- Measurement of Antibody Binding
Affinity on Bacterial Surfaces Using Flow Cytometry.- Detection of
Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected
with Group A Streptococcus.- In Vivo Profiling of the Vascular Cell Surface
Proteome in Murine Models of Bacteremia.- Barcoded Consortium Infections: A
Scalable, Internally Controlled Method to Study Host Cell Binding and
Invasion by Pathogenic Bacteria.- A Murine Mycobacterium marinum Infection
Model for Longitudinal Analyses of Disease Development and the Inflammatory
Response.- In Vitro Approaches for the Study of Pneumococcal-Neuronal
Interaction and Pathogenesis.