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E-grāmata: Basic Bioscience Laboratory Techniques: A Pocket Guide

4.17/5 (11 ratings by Goodreads)
(Nottingham Trent University), (Nottingham Trent University)
  • Formāts: EPUB+DRM
  • Izdošanas datums: 24-Aug-2011
  • Izdevniecība: John Wiley & Sons Inc
  • Valoda: eng
  • ISBN-13: 9781119956440
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Basic Bioscience Laboratory Techniques: A Pocket GuidePalielināt
  • Formāts: EPUB+DRM
  • Izdošanas datums: 24-Aug-2011
  • Izdevniecība: John Wiley & Sons Inc
  • Valoda: eng
  • ISBN-13: 9781119956440
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This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further reading All of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations.

In short, this is a 'must-have' for all first year bioscience students struggling to get to grips with this vitally important element of their course.

Recenzijas

"This would be a useful guide for those at the very beginning of their bioscience research career wanting in-depth information on very basic laboratory methods." (Doody's, 19 August 2011)

"Basic Bioscience Laboratory Techniques is a unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques." (Laboratory Journal, 10 March 2011)

Preface ix
Glossary xi
Abbreviations xvii
1 The preparation of solutions in bioscience research
1(26)
1.1 Introduction
1(1)
1.2 Concentration
2(1)
1.3 Using balances to weigh out reagents
3(3)
1.4 Practical considerations when making a 1.0 M solution
6(5)
1.5 Dilutions and the use of pipettes
11(4)
1.6 Water, acids and bases
15(6)
1.7 Buffers
21(3)
1.8 The equilibrium/dissociation constant (Ka) for an acid or base and the Henderson-Hasselbalch equation
24(2)
1.9 Summary
26(1)
2 Microscopy
27(24)
2.1 Introduction
27(2)
2.2 Microscopes - general principles
29(1)
2.3 Principles of image formation
30(2)
2.4 Light microscopy
32(14)
2.5 Electron microscopy
46(3)
2.6 Summary
49(2)
3 Spectrophotometry
51(23)
3.1 Introduction
51(1)
3.2 The electromagnetic spectrum
51(3)
3.3 The absorbance of light
54(2)
3.4 Absorbtion spectrophotometry
56(1)
3.5 The laws of the absorbance of light
57(4)
3.6 The Beer Lambert law
61(2)
3.7 Spectrophotometers
63(5)
3.8 Applications of spectrophotometry in bioscience
68(5)
3.9 Summary
73(1)
4 Data analysis and presentation
74(29)
4.1 Introduction
74(2)
4.2 Statistical analysis of data: some key definitions
76(3)
4.3 Distributions
79(6)
4.4 Statistical comparison of data
85(9)
4.5 Presentation, structure and organization of data in laboratory reports
94(6)
4.6 Summary
100(3)
5 The extraction and clarification of biological material
103(18)
5.1 General introduction
103(1)
5.2 Extraction
103(2)
5.3 Extraction methods for animal and plant tissue
105(2)
5.4 Extraction methods for bacteria
107(1)
5.5 Clarification
108(7)
5.6 Centrifugation techniques
115(4)
5.7 Points of good practice in centrifugation
119(1)
5.8 Summary
120(1)
6 Electrophoresis of proteins and nucleic acids
121(26)
6.1 General introduction
121(1)
6.2 Separation of protein mixtures by gel electrophoresis
122(14)
6.3 Other electrophoretic techniques applied to proteins
136(1)
6.4 Separation of nucleic acids by gel electrophoresis
137(4)
6.5 Applications of gel electrophoresis of nucleic acids
141(5)
6.6 Summary
146(1)
7 Chromatography
147(22)
7.1 General introduction
147(1)
7.2 The theory of chromatography
147(5)
7.3 Factors to consider in chromatography
152(1)
7.4 Methods used to elute samples in chromatography
153(1)
7.5 Different types of chromatography and what properties can be used to separate molecules
153(3)
7.6 Thin layer chromatography (TLC)
156(2)
7.7 High pressure liquid chromatography (HPLC)
158(3)
7.8 Gas liquid chromatography (GLC)
161(1)
7.9 Ion exchange chromatography (IEX)
162(2)
7.10 Size exclusion chromatography
164(2)
7.11 Affinity chromatography
166(1)
7.12 Summary
167(2)
8 Cell culture techniques
169(26)
8.1 Introduction
169(5)
8.2 Growth and maintenance of cells in culture
174(18)
8.3 Summary
192(3)
Suggestions for further reading 195(3)
Index 198
Dr Philip L.R. Bonner and Dr Alan J. Hargreaves, both of the School of Science and Technology, Nottingham Trent University.
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