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Chapter 1 Bioaugmentation for Ground Water Remediation: An Overview |
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1 | (38) |
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1 | (2) |
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1.1.1 Background: The Pollution Problem |
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1 | (1) |
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1.1.2 Definitions: General Bioremediation Terminology |
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2 | (1) |
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3 | (1) |
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1.2 Development of Bioaugmentation for Groundwater Bioremediation |
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3 | (3) |
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1.2.1 Historical Development of Bioaugmentation |
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3 | (1) |
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1.2.2 Recent Developments: Bioaugmentation with Dehalococcoides for Reductive Dehalogenation of Chlorinated Ethenes |
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4 | (2) |
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1.3 Types of Bioaugmentation |
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6 | (6) |
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1.3.1 Currently Practiced Methods |
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6 | (3) |
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1.3.2 Potential Bioaugmentation Strategies |
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9 | (3) |
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1.4 Making the Decision to Bioaugment |
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12 | (7) |
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1.4.1 Technical Analysis/Site Evaluation |
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14 | (1) |
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1.4.2 Select and Test Bioaugmentation Strategy |
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15 | (1) |
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1.4.3 Implement the Treatment |
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16 | (1) |
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1.4.4 Monitoring Effectiveness |
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17 | (2) |
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1.4.5 Other Considerations: Economics and Degradation Kinetics |
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19 | (1) |
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1.5 Bioaugmentation Issues |
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19 | (3) |
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1.5.1 Development of Effective Bioaugmentation Cultures |
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20 | (1) |
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1.5.2 Successful Inoculum Delivery and Dispersion |
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20 | (1) |
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20 | (1) |
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1.5.4 Pollutant Bioavailability |
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21 | (1) |
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1.5.5 Potential Undesirable Side-Effects |
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21 | (1) |
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1.6 Bioaugmentation to Remediate Chlorinated Compounds |
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22 | (2) |
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1.6.1 Chlorinated Aliphatic Hydrocarbons (CAHs): Dehalococcoides and the Chloroethenes |
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23 | (1) |
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1.6.2 Applications for Other Chlorinated Compounds |
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24 | (1) |
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1.7 Bioaugmentation to Remediate Other Contaminants |
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24 | (4) |
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25 | (1) |
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1.7.2 Polycyclic Aromatic Hydrocarbons (PAHs) |
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25 | (1) |
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1.7.3 Methyl Tert-Butyl Ether (MTBE) |
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26 | (1) |
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26 | (1) |
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27 | (1) |
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27 | (1) |
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28 | (11) |
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28 | (11) |
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Chapter 2 Dehalococcoides and Reductive Dechlorination of Chlorinated Solvents |
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39 | (50) |
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39 | (10) |
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2.1.1 The Chlorinated Ethene Problem |
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39 | (4) |
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2.1.2 Anaerobic Microbial Degradation of Chlorinated Ethenes |
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43 | (3) |
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2.1.3 Discovery of Dehalococcoides |
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46 | (3) |
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2.2 Dehalococcoides Isolation and Cultivation Strategies |
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49 | (3) |
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2.2.1 General Considerations |
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49 | (1) |
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50 | (1) |
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50 | (1) |
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51 | (1) |
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2.2.5 Reducing Agent (Reductant) |
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51 | (1) |
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2.2.6 Incubation Conditions |
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52 | (1) |
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52 | (1) |
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52 | (3) |
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2.3.1 Isolation of Dhc mccartyi Strain 195 |
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52 | (1) |
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2.3.2 Isolation of Dhc sp. Strain CBDB1 |
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53 | (1) |
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2.3.3 Isolation of Dhc sp. Strain FL2 |
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53 | (1) |
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2.3.4 Isolation of Dhc Strains That Respire VC: Strains BAV1, GT and VS |
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53 | (1) |
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2.3.5 Isolation of Dhc Strain MB |
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54 | (1) |
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2.4 Maintenance of Dehalococcoides Pure Cultures |
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55 | (1) |
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2.4.1 General Considerations |
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55 | (1) |
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55 | (1) |
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2.5 Dehalococcoides Morphology and Physiology |
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56 | (2) |
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2.6 Phylogeny of Dehalococcoides and Related Bacteria |
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58 | (3) |
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2.7 Dehalococcoides Genetics |
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61 | (3) |
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2.7.1 Insights from Dehalococcoides Genomes |
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61 | (2) |
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2.7.2 Dehalococcoides Reductive Dehalogenases Gene Operons |
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63 | (1) |
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2.8 Dehalococcoides Reductive Dehalogenases (RDASES) |
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64 | (1) |
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2.9 Biochemistry of Reductive Dechlorination by Dehalococcoides |
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65 | (2) |
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2.10 Dehalococcoides Biomarkers |
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67 | (5) |
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2.11 Dehalococcoides Evolution and Dissemination of Reductive Dehalogenase Genes |
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72 | (1) |
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2.12 Dehalococcoides Biogeography |
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73 | (1) |
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2.13 Dehalococcoides Ecology |
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74 | (1) |
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75 | (1) |
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2.15 Implications for Bioremediation Practice: Take Home Messages |
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76 | (13) |
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76 | (13) |
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Chapter 3 Production and Handling of Dehalococcoides Bioaugmentation Cultures |
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89 | (28) |
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89 | (2) |
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3.1.1 Microbial Cultures Used for Bioaugmentation |
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89 | (2) |
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3.1.2 Why High Density Microbial Cultures Are Important |
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91 | (1) |
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91 | (4) |
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3.2.1 Microbial Growth Options: Batch Versus Continuous |
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91 | (2) |
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3.2.2 Culture Growth Protocol |
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93 | (2) |
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3.3 Full-Scale Production Results |
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95 | (9) |
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3.3.1 Factors Affecting Culture Growth |
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99 | (5) |
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3.4 Quality Assurance/Quality Control Considerations |
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104 | (2) |
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104 | (1) |
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104 | (1) |
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105 | (1) |
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3.4.4 Other QA/QC Considerations |
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106 | (1) |
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3.5 Concentrating and Storing Inocula |
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106 | (4) |
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3.5.1 Concentrating Cultures |
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107 | (2) |
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3.5.2 Culture Stability and Storage |
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109 | (1) |
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110 | (1) |
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111 | (2) |
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111 | (1) |
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112 | (1) |
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3.7.3 Mixing with Other Reagents Before Injection |
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112 | (1) |
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113 | (4) |
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113 | (4) |
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Chapter 4 Bioaugmentation With Dehalococcoides: A Decision Guide |
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117 | (24) |
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117 | (1) |
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4.2 Need for Decision Guidance |
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118 | (1) |
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4.3 Decision Guidance Overview |
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119 | (1) |
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4.4 Is Complete Dechlorination Occurring? |
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119 | (2) |
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4.5 Are the Site Conditions Inhibitory? |
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121 | (1) |
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4.6 Is the Site Highly Aerobic? |
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122 | (1) |
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4.7 Will Biostimulation Work? |
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123 | (8) |
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4.7.1 Laboratory Diagnostic Tests |
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124 | (4) |
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128 | (3) |
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4.8 How Valuable is Time? |
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131 | (1) |
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4.9 Is the Risk of Exposure to Toxic Intermediates Unacceptable? |
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132 | (1) |
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4.10 Economic Assessments of Bioaugmentation |
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132 | (2) |
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4.11 Summary and Recommendations |
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134 | (7) |
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135 | (6) |
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Chapter 5 Bioaugmentation Considerations |
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141 | (30) |
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141 | (1) |
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5.2 Effect of Site Conditions on Effectiveness of Bioaugmentation |
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141 | (3) |
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141 | (1) |
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142 | (1) |
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5.2.3 Competition for Electron Donor/Geochemical Conditions |
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142 | (1) |
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5.2.4 Volatile Organic Compound (VOC) Concentration |
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143 | (1) |
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5.2.5 Inhibitory Constituents |
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143 | (1) |
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144 | (1) |
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144 | (9) |
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5.3.1 Injection Infrastructure Considerations |
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144 | (1) |
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5.3.2 Preconditioning Requirements |
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145 | (3) |
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5.3.3 Culture Requirements |
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148 | (1) |
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5.3.4 Injection Techniques |
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148 | (4) |
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5.3.5 Distribution Techniques |
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152 | (1) |
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5.4 Bioremediation Configurations Employing Bioaugmentation |
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153 | (8) |
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5.4.1 Active Recirculation Approach |
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153 | (6) |
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5.4.2 Semi-Passive Approach |
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159 | (1) |
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160 | (1) |
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161 | (10) |
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162 | (1) |
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Appendix 5A Background on Innoculum Density and Dechlorination Rates |
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162 | (9) |
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Chapter 6 Microbial Monitoring During Bioaugmentation with Dehalococcoides |
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171 | (28) |
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171 | (2) |
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6.2 MBTs for Chlorinated Ethene Biodegradation |
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173 | (1) |
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6.3 Developing a Monitoring Strategy |
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174 | (1) |
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6.3.1 Defining Monitoring Objectives |
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174 | (1) |
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6.3.2 Temporal Considerations |
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174 | (1) |
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6.3.3 Selection of Sampling Wells |
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174 | (1) |
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175 | (5) |
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6.4.1 General Sampling Considerations |
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175 | (2) |
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6.4.2 Groundwater Sampling Protocol |
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177 | (3) |
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180 | (5) |
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6.5.1 Description and General Methodology |
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180 | (2) |
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182 | (1) |
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182 | (2) |
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184 | (1) |
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185 | (1) |
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6.6 Fluorescent In Situ Hybridization |
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185 | (4) |
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185 | (1) |
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6.6.2 Description and General Methodology |
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186 | (1) |
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187 | (1) |
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6.6.4 Conjunctive Technologies |
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188 | (1) |
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188 | (1) |
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189 | (4) |
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6.7.1 Gel Electrophoresis |
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189 | (1) |
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6.7.2 Cloning and Sequencing |
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189 | (1) |
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6.7.3 Terminal-Restriction Fragment Length Polymorphism |
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190 | (1) |
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6.7.4 Denaturing Gel Gradient Electrophoresis |
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190 | (1) |
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6.7.5 Temperature Gel Gradient Electrophoresis |
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190 | (1) |
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6.7.6 Microarrays and High-Throughput Sequencing |
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191 | (1) |
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192 | (1) |
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6.8 Data Evaluation and Interpretation of MBTs |
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193 | (1) |
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6.9 Future Research Needs |
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194 | (5) |
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194 | (5) |
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Chapter 7 Bioaugmentation For Aerobic Degradation Of CIS-1,2-Dichloroethene |
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199 | (20) |
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199 | (1) |
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7.2 Polaromonas sp. Strain JS666 |
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200 | (7) |
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201 | (1) |
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7.2.2 Kinetics, Thresholds and Tolerances to cis-DCE and Oxygen |
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201 | (1) |
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7.2.3 Insight About Metabolic Pathways from Genomics and Proteomics |
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202 | (2) |
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7.2.4 Cometabolism of Other Chlorinated Solvents |
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204 | (1) |
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7.2.5 Development of a Molecular Probe for Process Monitoring |
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205 | (1) |
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7.2.6 Development of Strategy for Growth of Inocula |
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206 | (1) |
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7.3 Microcosm Assessment of Site-Suitability |
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207 | (1) |
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7.3.1 Microcosm Preparation |
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207 | (1) |
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7.3.2 Previous Experiences with Microcosm Assessment |
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208 | (1) |
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208 | (4) |
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7.4.1 Test Site Selection |
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208 | (1) |
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7.4.2 Preliminary Microcosm Study |
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209 | (1) |
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7.4.3 Titration Studies with SJCA Groundwater |
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210 | (1) |
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210 | (2) |
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7.5 Summary and Future Prospects |
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212 | (7) |
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213 | (6) |
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Chapter 8 Bioaugmentation for the In Situ Aerobic Cometabolism of Chlorinated Solvents |
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219 | (38) |
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219 | (1) |
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8.2 Aerobic Cometabolic Processes |
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219 | (3) |
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8.3 Aerobic Cometabolism by Indigenous Microorganisms |
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222 | (6) |
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8.3.1 Microcosm Studies with Indigenous Microorganisms |
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222 | (1) |
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8.3.2 Field Studies with Indigenous Microorganisms |
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222 | (6) |
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8.4 Bioaugmentation Approaches |
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228 | (21) |
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8.4.1 Bioaugmentation Approach I |
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228 | (9) |
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8.4.2 Bioaugmentation Approach II |
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237 | (5) |
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8.4.3 Bioaugmentation Approach III |
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242 | (6) |
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8.4.4 Bioaugmentation Approach IV |
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248 | (1) |
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249 | (8) |
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251 | (6) |
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Chapter 9 Bioaugmentation with Pseudomonas Stutzeri KC for Carbon Tetrachloride Remediation |
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257 | (32) |
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9.1 Introduction and Rationale |
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257 | (1) |
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9.2 Physiological Function of PDTC Production |
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258 | (1) |
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9.3 CT Transformation by P. Stutzeri KC as a Novel Dechlorination Reaction |
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259 | (4) |
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9.3.1 Pathway of PDTC-Promoted CT Dechlorination |
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260 | (1) |
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9.3.2 Transition Metal Chelation of PDTC |
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261 | (2) |
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9.4 Genetic Requirements for PDTC Production |
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263 | (2) |
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9.5 PDTC-Mediated CT Transformation |
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265 | (4) |
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266 | (1) |
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9.5.2 Cell and CT Concentration |
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266 | (2) |
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9.5.3 Cell Membrane Components |
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268 | (1) |
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9.5.4 An Overall Model for CT Transformation by Pseudomonas stutzeri KC |
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269 | (1) |
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9.6 Bioaugmentation with P. Stutzeri KC: Transport, Growth and Competition |
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269 | (4) |
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9.6.1 Inoculation and Transport |
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270 | (1) |
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9.6.2 Growth and Competition |
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271 | (2) |
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273 | (2) |
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9.8 Field Experience: Pilot- and Demonstration-Scale Testing |
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275 | (8) |
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9.8.1 Design and Site Characterization |
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275 | (1) |
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9.8.2 pH of Adjustment, Inoculation and Biocurtain Colonization |
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275 | (4) |
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9.8.3 Long-Term Maintenance of the Biocurtain |
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279 | (4) |
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9.9 Future use of Pseudomonas Stutzeri KC and PDTC |
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283 | (6) |
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285 | (4) |
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Chapter 10 Bioaugmentation for MTBE Remediation |
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289 | (24) |
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289 | (1) |
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10.2 MTBE Use and Occurrence in Groundwater |
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289 | (1) |
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10.3 Scientific Basis for Bioaugmentation of MTBE and TBA |
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290 | (6) |
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10.3.1 MTBE Degrading Bacteria |
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290 | (3) |
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10.3.2 MTBE and TBA Biodegradation in Microcosms |
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293 | (1) |
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10.3.3 Evaluating MTBE and TBA Biodegradation |
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293 | (3) |
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10.4 Bioaugmentation Pilot Testing |
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296 | (7) |
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10.4.1 Bioaugmentation with Direct Degraders (MC-100 and SC-100) |
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296 | (6) |
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10.4.2 Bioaugmentation with Direct Degraders (PM1) |
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302 | (1) |
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10.4.3 Bioaugmentation with Propane Oxidizers (ENV 425) |
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302 | (1) |
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10.5 Full Scale Bioaugmentation |
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303 | (2) |
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10.5.1 MC-100 and SC-100 (Port Hueneme, California, USA) |
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303 | (2) |
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10.5.2 MC-100 (Connecticut, USA) |
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305 | (1) |
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10.5.3 MC-100 (California, USA) |
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305 | (1) |
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10.5.4 Propane Oxidizing Bacteria (Camden, New Jersey, USA) |
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305 | (1) |
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305 | (2) |
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307 | (1) |
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10.8 Future Prospects for MTBE Bioaugmentation |
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308 | (5) |
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308 | (5) |
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Chapter 11 Economics and Valuation of Bioaugmentation |
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313 | (20) |
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313 | (1) |
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11.2 Primary Cost Drivers |
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313 | (5) |
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11.2.1 Site Specific Testing to Evaluate Bioaugmentation |
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314 | (1) |
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11.2.2 Amount and Distribution of Active Organisms |
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315 | (3) |
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11.3 Costs, Value and Benefits of Bioaugmentation |
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318 | (2) |
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11.3.1 Costs for Bioaugmentation Culture and Injection |
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318 | (1) |
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11.3.2 Value of Bioaugmentation Relative to a "Wait and See" Approach to Degradation of DCE and VC |
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319 | (1) |
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11.4 Economics of Alternative Approaches |
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320 | (1) |
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11.4.1 Costs for Purchase and Injection of Concentrate Versus In Situ Growth and Distribution |
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320 | (1) |
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11.5 Estimated Costs for Template Scenarios |
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321 | (9) |
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11.5.1 Template Site Descriptions |
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321 | (2) |
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11.5.2 Costs Categories and Components |
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323 | (1) |
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11.5.3 EISB Remediation Technology Description |
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324 | (2) |
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11.5.4 EISB Remediation Technology Costs |
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326 | (4) |
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330 | (3) |
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331 | (2) |
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Chapter 12 Research Needs for Bioaugmentation |
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333 | (30) |
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333 | (1) |
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12.2 Research Needs in Basic Science |
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333 | (8) |
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334 | (1) |
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335 | (4) |
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339 | (1) |
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12.2.4 Environmental/Ecosystem and Earth Scale |
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339 | (2) |
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12.3 Key Concepts for Bioaugmentation Research |
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341 | (8) |
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12.3.1 The Niche Concept and Its Importance for Bioaugmentation |
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341 | (2) |
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12.3.2 Hydrocarbons and Other Reduced Contaminants |
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343 | (2) |
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12.3.3 The Much-Maligned Microcosm and the Need for Activity-Based Tests |
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345 | (1) |
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12.3.4 The Enrichment Paradox |
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346 | (3) |
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12.4 Applied Research Needs |
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349 | (2) |
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349 | (1) |
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12.4.2 Production, Storage and Shipping |
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350 | (1) |
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12.4.3 Delivery and Mixing |
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350 | (1) |
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12.4.4 Electron Donor Choice |
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350 | (1) |
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12.4.5 Regulatory Considerations |
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351 | (1) |
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12.4.6 Modeling of Sites, Dechlorination and Biological Activity |
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351 | (1) |
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351 | (5) |
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12.5.1 Biosensors as MBTs |
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352 | (1) |
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12.5.2 Designer Microbes and Synthetic Biology |
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352 | (1) |
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12.5.3 Bioaugmenting with Genes |
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353 | (1) |
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12.5.4 Bioaugmenting with Viruses |
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354 | (2) |
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356 | (7) |
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356 | (7) |
Appendix A List of Acronyms, Abbreviations and Symbols |
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363 | (4) |
Appendix B Unit Conversion Table |
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367 | (2) |
Appendix C Glossary |
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369 | (16) |
Index |
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385 | |