Author Bio |
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Chapter 1 Buffers |
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1 | (8) |
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2 | (2) |
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Method 1: A Free Acid Titrated by NaOH |
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2 | (1) |
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Method 2: A Free Base Titrated by HCl |
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2 | (1) |
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Method 3: Adding Free Acid and Free Base to Make the Buffer |
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3 | (1) |
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4 | (1) |
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5 | (1) |
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6 | (1) |
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6 | (1) |
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7 | (2) |
Chapter 2 Assays |
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9 | (12) |
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1 Lactate Dehydrogenase: Assay by Substrates Alone |
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9 | (1) |
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2 Phosphofructokinase: Assay by Metabolic Pathway |
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10 | (2) |
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3 Assay by Immunochemistry |
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12 | (1) |
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4 Catalase: Assay by Physical Properties |
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12 | (7) |
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Ways of Reporting Enzyme Activity |
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13 | (1) |
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Evaluating a Source for an Enzyme |
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14 | (1) |
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14 | (1) |
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15 | (1) |
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15 | (1) |
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16 | (3) |
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19 | (1) |
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20 | (1) |
Chapter 3 Protein Concentration |
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21 | (10) |
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21 | (1) |
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22 | (1) |
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3 Bicinchionic Acid Assay |
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23 | (1) |
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Finding the Concentration |
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23 | (5) |
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26 | (1) |
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26 | (1) |
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26 | (2) |
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Recipe for Bradford Reagent |
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28 | (1) |
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28 | (1) |
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29 | (2) |
Chapter 4 ELISA |
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31 | (6) |
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33 | (1) |
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33 | (1) |
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33 | (1) |
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34 | (1) |
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35 | (2) |
Chapter 5 Salting Out Proteins and Other Biomolecules |
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37 | (8) |
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40 | (1) |
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40 | (1) |
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41 | (1) |
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42 | (1) |
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42 | (3) |
Chapter 6 A Discussion of Isoelectric Point and Effective Charge |
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45 | (8) |
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45 | (2) |
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47 | (3) |
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50 | (3) |
Chapter 7 Column Chromatography |
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53 | (16) |
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54 | (5) |
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59 | (1) |
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60 | (5) |
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65 | (1) |
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66 | (3) |
Chapter 8 Michaelis-Menten Kinetics |
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69 | (12) |
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73 | (2) |
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75 | (3) |
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75 | (1) |
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76 | (9) |
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76 | (2) |
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78 | (1) |
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79 | (2) |
Chapter 9 Protein Purification |
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81 | (8) |
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81 | (1) |
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82 | (3) |
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85 | (1) |
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85 | (1) |
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85 | (1) |
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86 | (1) |
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86 | (1) |
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86 | (1) |
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87 | (2) |
Chapter 10 Polyacrylamide Gels |
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89 | (12) |
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Acrylamide Chemistry Affects Separations |
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89 | (2) |
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91 | (2) |
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92 | (1) |
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92 | (1) |
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Loading and Running an SDS-PAGE Gel |
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93 | (3) |
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93 | (1) |
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94 | (2) |
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96 | (2) |
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98 | (3) |
Chapter 11 In Silico Biochemistry: The Evolution of Globins |
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101 | (14) |
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101 | (1) |
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102 | (4) |
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Aligning and Scoring Sequences |
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106 | (1) |
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106 | (1) |
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107 | (1) |
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Calculating Evolutionary Distance |
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108 | (6) |
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111 | (3) |
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114 | (1) |
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114 | (1) |
Chapter 12 Growing Crystals of Hemoglobin |
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115 | (12) |
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Hanging Drop and Sitting Drop Methods |
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116 | (3) |
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119 | (1) |
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120 | (1) |
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121 | (5) |
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121 | (12) |
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121 | (5) |
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126 | (1) |
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126 | (1) |
Chapter 13 Enzyme Inhibition |
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127 | (10) |
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127 | (2) |
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129 | (2) |
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131 | (1) |
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132 | (1) |
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133 | (3) |
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134 | (2) |
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136 | (1) |
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136 | (1) |
Chapter 14 Multisubstrate Kinetics |
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137 | (8) |
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137 | (2) |
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139 | (1) |
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140 | (3) |
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140 | (3) |
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143 | (2) |
Chapter 15 Fluorescence and Denaturation |
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145 | (14) |
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145 | (2) |
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Structural Studies by Fluorescence |
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147 | (3) |
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Fluorescence Resonance Energy Transfer (FRET) |
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150 | (1) |
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FRET Studies for Unfolding |
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151 | (1) |
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Experimental Design Considerations |
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151 | (1) |
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151 | (1) |
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151 | (2) |
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Using an Unfolding Curve to Find Kfold and ΔGfold |
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153 | (3) |
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156 | (2) |
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156 | (1) |
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156 | (2) |
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158 | (1) |
Chapter 16 Fluorescence Studies of Ligand Binding |
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159 | (10) |
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Experimental Considerations |
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161 | (3) |
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161 | (1) |
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161 | (1) |
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Fitting Data to a Loss of Intensity |
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161 | (3) |
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164 | (3) |
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164 | (1) |
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165 | (2) |
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167 | (1) |
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167 | (1) |
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168 | (1) |
Chapter 17 DNA Restriction Digests |
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169 | (10) |
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169 | (2) |
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Restriction Endonucleases |
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171 | (1) |
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Plasmid Types and Purposes |
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172 | (1) |
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173 | (3) |
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173 | (1) |
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174 | (2) |
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176 | (3) |
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Tutorial: How to Construct a Restriction Map, Logically |
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176 | (3) |
Chapter 18 Western Blotting |
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179 | (6) |
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181 | (3) |
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181 | (1) |
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181 | (1) |
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181 | (1) |
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182 | (1) |
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182 | (1) |
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182 | (2) |
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Part 1: Native Gel Electrophoresis |
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182 | (1) |
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183 | (1) |
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183 | (1) |
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184 | (1) |
Index |
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185 | |