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Biomedical Science Practice 2nd Revised edition [Mīkstie vāki]

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Edited by (School of Healthcare Science, Manchester Metropolitan University), Edited by (School of Healthcare Science, Manchester Metropolitan University), Edited by (Advanced Specialist Biomedical Scientist, Cytology Department, Queen Alexandra Hospital)
  • Formāts: Paperback / softback, 512 pages, height x width x depth: 246x193x21 mm, weight: 828 g, 270 colour line drawings and photographs
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 14-Jul-2016
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0198717318
  • ISBN-13: 9780198717317
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Biomedical Science Practice 2nd Revised editionPalielināt
  • Formāts: Paperback / softback, 512 pages, height x width x depth: 246x193x21 mm, weight: 828 g, 270 colour line drawings and photographs
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 14-Jul-2016
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0198717318
  • ISBN-13: 9780198717317
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9780198717317.html
Keywords:
Biomedical scientists are the foundation of modern healthcare, from cancer screening to diagnosing HIV, from blood transfusion for surgery to food poisoning and infection control. Without biomedical scientists, the diagnosis of disease, the evaluation of the effectiveness of treatment, and research into the causes and cures of disease would not be possible.

The Fundamentals of Biomedical Science series has been written to reflect the challenges of practicing biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analytical approaches that lead to diagnosis. Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a Biomedical Scientist may be exposed - from microbiology to cytopathology to transfusion science.

A core text in the Fundamentals of Biomedical Science series, Biomedical Science Practice gives a comprehensive overview of the key laboratory techniques and professional skills that students need to master. The text is supported throughout with engaging clinical case studies, written to emphasize the link between theory and practice, providing a strong foundation for beginning biomedical science students.
Dedication ix
An introduction to the Fundamentals of Biomedical Science series x
Preface xv
Contributors xvii
Abbreviations xix
1 Biomedical science and biomedical scientists
1(17)
Hedley Glencross
Introduction
1(1)
1.1 What is biomedical science?
2(1)
1.2 Biomedical science degree programmes
3(1)
1.3 What is a biomedical scientist?
4(2)
1.4 Professional skills
6(1)
1.5 Initial employment as a biomedical scientist
6(2)
1.6 What do biomedical scientists do?
8(10)
Summary
16(1)
Further reading
16(1)
Questions
16(2)
2 Fitness to practise
18(18)
Hedley Glencross
Introduction
18(1)
2.1 Healthcare regulation
18(1)
2.2 Health and Care Professions Council
19(3)
2.3 Health and Care Professions Council Standards of Conduct, Performance and Ethics
22(3)
2.4 Renewal of Health and Care Professions Council registration
25(1)
2.5 Institute of Biomedical Science
26(1)
2.6 Institute of Biomedical Science Code of Conduct and Good Professional Practice
27(2)
2.7 Institute of Biomedical Science and education
29(7)
Summary
34(1)
Further reading
34(1)
Questions
35(1)
3 Communications in laboratory medicine
36(20)
Hedley Glencross
Georgina Lavender
Introduction
36(1)
3.1 Communication and the clinical laboratory
37(4)
3.2 Communication and confidentiality
41(1)
3.3 Methods of communication
42(4)
3.4 Data protection
46(5)
3.5 Recordkeeping
51(5)
Summary
53(1)
Further reading
54(1)
Questions
54(2)
4 Health and safety
56(26)
Hedley Glencross
Alison Taylor
Introduction
56(1)
4.1 Hazards
57(2)
4.2 Routes of entry into the body by chemical and biological agents
59(1)
4.3 Statutory framework for health and safety
59(5)
4.4 Risk assessment
64(4)
4.5 Control of Substances Hazardous to Health
68(2)
4.6 Fire regulations
70(2)
4.7 Personal protective equipment
72(2)
4.8 Reporting of Injuries, Diseases and Dangerous Occurrences Regulations
74(1)
4.9 Transport regulations
74(1)
4.10 Personal health and safety
75(3)
4.11 Universal or standard precautions
78(4)
Summary
79(1)
Further reading
79(1)
Questions
80(2)
5 Preparing and measuring reagents
82(23)
Ian Graham
Introduction
82(1)
5.1 Balances and weighing
83(4)
5.2 Volume measurements and delivery
87(4)
5.3 Pipettors
91(4)
5.4 Preparing reagents: concentrations and dilutions
95(10)
Summary
103(1)
Further reading
103(1)
Questions
104(1)
6 Samples and sample collection
105(25)
Joyce Overfield
Introduction
105(1)
6.1 Blood
106(8)
6.2 Urine
114(2)
6.3 Fluids other than blood and urine
116(3)
6.4 Cytopathology and histopathology samples
119(2)
6.5 Samples for microbiology testing
121(9)
Summary
127(1)
Further reading
128(1)
Questions
129(1)
7 Microscopy
130(30)
Tony Sims
Qiuyu Wang
Introduction
130(1)
7.1 Microscopy and image formation
131(6)
7.2 Components of a microscope
137(4)
7.3 Magnification
141(1)
7.4 Dark field microscopy
141(1)
7.5 Phase contrast microscopy
142(1)
7.6 Polarization microscopy
143(1)
7.7 Fluorescence microscopy
144(3)
7.8 Confocal microscopy
147(1)
7.9 Inverted microscopes
148(1)
7.10 Electron microscopy
149(11)
Summary
157(1)
Further reading
158(1)
Questions
158(2)
8 Electrochemistry
160(22)
Peter Robinson
Introduction
160(1)
8.1 Basic concepts and definitions
161(1)
8.2 Principles of electrochemical techniques
162(2)
8.3 Potentiometric techniques
164(8)
8.4 Voltammetric techniques
172(2)
8.5 Biosensors
174(8)
Summary
179(1)
Further reading
179(2)
Questions
181(1)
9 Spectroscopy
182(30)
Qiuyu Wang
Helen Montgomery
Nessar Ahmed
Chris Smith
Introduction
182(1)
9.1 Radiation and the electromagnetic spectrum
182(2)
9.2 Interactions between radiation and matter
184(1)
9.3 Radiation, particles, and quanta
185(1)
9.4 Absorbance
186(4)
9.5 Absorption spectra
190(5)
9.6 Light scattering methods
195(1)
9.7 Fluorescence and fluorimetry
196(1)
9.8 Nuclear magnetic resonance spectroscopy
197(5)
9.9 Mass spectrometry
202(10)
Summary
209(1)
Further reading
210(1)
Questions
211(1)
10 Centrifugation
212(19)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
212(1)
10.1 Basics of centrifugation theory
213(4)
10.2 Types of centrifuges
217(2)
10.3 Tubes and rotors
219(2)
10.4 Separation methods using centrifuges
221(3)
10.5 Safety and centrifuges
224(1)
10.6 Examples of clinical centrifugation
225(6)
Summary
228(1)
Further reading
229(1)
Questions
229(2)
11 Chromatography
231(25)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
231(1)
11.1 Partition or distribution coefficient
232(1)
11.2 Planar chromatography
233(3)
11.3 Column chromatography
236(9)
11.4 High-performance liquid chromatography
245(4)
11.5 Gas-liquid chromatography
249(7)
Summary
253(1)
Further reading
253(1)
Questions
254(2)
12 Electrophoresis
256(31)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
256(1)
12.1 Principles of electrophoresis
256(2)
12.2 Factors affecting electrophoretic separations
258(2)
12.3 Detection of separated components
260(2)
12.4 Paper or cellulose electrophoresis
262(1)
12.5 Cellulose acetate electrophoresis
263(2)
12.6 Starch electrophoresis
265(1)
12.7 Polyacrylamide gel electrophoresis
265(8)
12.8 Agarose gel electrophoresis
273(4)
12.9 Capillary electrophoresis
277(3)
12.10 Isoelectric focusing
280(2)
12.11 Two-dimensional electrophoresis
282(5)
Summary
284(1)
Further reading
284(1)
Questions
285(2)
13 Immunological techniques
287(31)
Christine Yates
Qiuyu Wang
Introduction
287(1)
13.1 Outline of the immune system
288(2)
13.2 Synthesis of antibodies in vivo
290(2)
13.3 Production of polyclonal and monoclonal antibodies in vitro
292(2)
13.4 Antigen-antibody interactions
294(1)
13.5 Immunological techniques used in clinical laboratories
295(23)
Summary
316(1)
Further reading
316(1)
Questions
317(1)
14 Molecular biology techniques
318(44)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
318(1)
14.1 Structure and properties of macromolecules
319(8)
14.2 Molecular biology-based techniques
327(1)
14.3 Isolation of nucleic acids
327(5)
14.4 Hydrolysis of nucleic acids and restriction endonucleases
332(4)
14.5 Gel electrophoresis
336(1)
14.6 DNA sequencing
337(4)
14.7 Blotting techniques
341(6)
14.8 Enzyme-linked immunosorbent assays and fluorescence in situ hybridization
347(2)
14.9 DNA cloning
349(7)
14.10 DNA microarrays
356(6)
Summary
359(1)
Further reading
360(1)
Questions
361(1)
15 Laboratory automation
362(15)
Tim James
Introduction
362(1)
15.1 Benefits of automation
363(2)
15.2 Collection of suitable samples and delivery to the laboratory
365(2)
15.3 Specimen reception considerations in the core automated laboratory
367(2)
15.4 Tracked automation systems and the core automated laboratory
369(4)
15.5 Automation in wider laboratory settings
373(4)
Summary
374(1)
Further reading
375(1)
Questions
375(2)
16 Point-of-care testing
377(23)
Jan Still
Lynda Petley
Garry McDowell
Introduction
377(1)
16.1 Standards and guidelines
378(1)
16.2 Advantages and limitations of point-of-care testing
378(4)
16.3 Point-of-care committee and point-of-care policy
382(3)
16.4 Procurement
385(1)
16.5 Evaluating a point-of-care device
386(1)
16.6 Use of point-of-care devices
387(2)
16.7 Connectivity
389(1)
16.8 Training for point-of-care
389(1)
16.9 Quality and point-of-care
390(1)
16.10 Audit
391(1)
16.11 Problems, incidents, and litigation
392(8)
Summary
397(1)
Further reading
397(1)
Questions
398(2)
17 Quality assurance and management
400(30)
Elaine Moore
Introduction
400(1)
17.1 Why is quality important?
401(1)
17.2 Quality in the laboratory
401(6)
17.3 Audit and self-assessment
407(4)
17.4 Regulatory requirements
411(5)
17.5 Quality governance and risk management
416(7)
17.6 Patient safety
423(3)
17.7 Tools and techniques for continual improvement
426(4)
Summary
427(1)
Further reading
428(1)
Questions
429(1)
18 Personal development
430(38)
Hedley Glencross
Georgina Lavender
Introduction
430(2)
18.1 Continuing professional development
432(2)
18.2 Opportunities for personal development in the workplace
434(2)
18.3 Training and development
436(1)
18.4 Professional bodies and personal development
437(5)
18.5 Commercial organizations and personal development
442(1)
18.6 Opportunities for self-guided personal development
443(1)
18.7 Reflective learning
443(1)
18.8 Study skills
444(5)
18.9 Evidence supporting personal development
449(1)
18.10 Performance appraisal and personal development plans
450(18)
Summary
451(1)
Further reading
451(1)
Questions
452(2)
Answers to self-check questions
454(5)
Answers to case studies
459(2)
Answers to end-of-chapter questions
461(7)
Glossary 468(9)
Index 477
Dr Nessar Ahmed is a Reader in Clinical Biochemistry at the Manchester Metropolitan University where his research examines the role of protein glycation in diabetes. His teaching includes clinical biochemistry, endocrinology, and analytical science.

Hedley Glencross is an Advanced Specialist Biomedical Scientist at the Cytology Department, Queen Alexandra Hospital, Portsmouth, where his special interest is in diagnostic (non-gynaecological cytology. He is an elected member of the Executive Committee of the British Association for Cytopathology and is also an assessor for UK NEQAS.

Dr Qiuyu Wang is a Senior Lecturer in Molecular and Cell Biology at the Manchester Metropolitan University. Her major research interest is in cancer biology and stem cell biology. She teaches many aspects of Molecular and Cellular Biology.