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Biomedical Science Practice: Experimental and Professional Skills [Mīkstie vāki]

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  • Formāts: Paperback / softback, 608 pages, height x width x depth: 246x190x26 mm, weight: 1298 g, 200 colour
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 14-Oct-2010
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0199533296
  • ISBN-13: 9780199533299
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  • Formāts: Paperback / softback, 608 pages, height x width x depth: 246x190x26 mm, weight: 1298 g, 200 colour
  • Sērija : Fundamentals of Biomedical Science
  • Izdošanas datums: 14-Oct-2010
  • Izdevniecība: Oxford University Press
  • ISBN-10: 0199533296
  • ISBN-13: 9780199533299
Citas grāmatas par šo tēmu:
The Fundamentals Of Biomedical Science Series is written to reflect the challenges of practising biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analytical approaches that lead to diagnosis.

A blend of science theory and biomedical science practice make this series ideal for those seeking both the knowledge and skills to become proficient Biomedical Scientists.

Content carefully developed to help you demonstrate the competencies required during the study of biomedical science at both undergraduate and postgraduate level.

Case studies and other examples enrich the text, firmly rooting it in the context of clinical and biomedical practice.

Biomedical scientists are the foundation of modern healthcare, from cancer screening to diagnosing HIV, from blood transfusion for surgery to food poisoning and infection control. Without biomedical scientists, the diagnosis of disease, the evaluation of the effectiveness of treatment, and research into the causes and cures of disease would not be possible.

The Fundamentals of Biomedical Science series is written to reflect the challenges of practicing biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analytical approaches that lead to diagnosis.

Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a Biomedical Scientist may be exposed - from microbiology to cytopathology to transfusion science. Alongside volumes exploring specific biomedical themes and related laboratory diagnosis, an overarching Biomedical Science Practice volume gives a grounding in the professional and experimental skills with which any Biomedical Scientist must be equipped.

The series
DT Understands the complex roles of Biomedical Scientists in the modern practice of medicine.
DT Understands the development needs of employers and the Profession.
DT Addresses the need for understanding of a range of fundamental sciences in the context of Biomedicine.
DT Places the theoretical aspects of Biomedical Science in their practical context.

Biomedical Science Practice presents the essential practical and professional skills that every biomedical scientist should master, making it the perfect foundation for the study of each of the key subject specialisms that maybe encountered in the biomedical lab.

The book reviews a broad range of professional skills and concepts, including health and safety considerations, personal development, and communication, and also introduces the key experimental and analytical approaches that form the basis of the investigation and diagnosis of clinical conditions.

Drawing on the wealth of experience of a range of biomedical science practitioners and educators, Biomedical Science Practice is the ideal companion throughout your biomedical science education and training.


Online Resource Centre
The Online Resource Centre to accompany Biomedical Science Practice features:

For registered adopters of the book:
DT Figures from the book, available to download

For students:
DT An interactive Digital Microscope, encouraging the exploration of tissue samples
DT Self-assessment modules to help you to check and reinforce understanding of the basic science introduced in each volume
DT Video podcasts including interviews with practicing biomedical scientists, and 'in the lab' footage showing biomedical science in practice

Recenzijas

A highly useful resource that brings together a range of specialty knowledge that would not normally be found in one text. Nathaniel Milton, Roehampton University Challenges students on a scientific note and should inspire the more confident to really think about what is going on in the diagnostic laboratory and the technical pros and cons associated with various techniques. Glenn Hussey, Keele University I think the idea of a book covering professional practice is invaluable to both the undergraduate student wishing to follow a career as a Biomedical Scientist and a postgraduate recruit to the profession who is about to start the training portfolio. Gillian Campling, Durham University

An introduction to the Fundamentals of Biomedical Science series x
Acknowledgements xiv
Contributors xv
Abbreviations xvi
1 Biomedical science and biomedical scientists 1(19)
Hedley Glencross
Introduction
1(1)
1.1 What is biomedical science?
2(1)
1.2 Biomedical science degree programmes
3(1)
1.3 What is a biomedical scientist?
4(2)
1.4 Professional skills
6(4)
1.5 Initial employment as a biomedical scientist
10(2)
1.6 What do biomedical scientists do?
12(6)
Summary
18(1)
Further reading
19(1)
Questions
19(1)
2 Fitness to practise 20(22)
Hedley Glencross
Introduction
20(1)
2.1 Healthcare regulation
20(1)
2.2 Health Professions Council
21(3)
2.3 HPC Standards of Conduct, Performance and Ethics
24(6)
2.4 Renewal of HPC registration
30(1)
2.5 Institute of Biomedical Science
30(1)
2.6 IBMS Code of Conduct and Good Professional Practice
31(3)
2.7 IBMS and education
34(5)
Summary
39(1)
Further Reading
39(1)
Questions
40(2)
3 Communications in laboratory medicine 42(24)
Georgina Lavender
Introduction
42(1)
3.1 Communication and the clinical laboratory
43(5)
3.2 Communication and confidentiality
48(1)
3.3 Methods of communication
49(6)
3.4 Data protection
55(6)
3.5 Record keeping
61(2)
Summary
63(1)
Further Reading
63(1)
Questions
64(2)
4 Health and safety 66(29)
Alison Taylor
Introduction
66(1)
4.1 Hazards
67(3)
4.2 Routes of entry into the body by chemical and biological agents
70(1)
4.3 Statutory framework for health and safety
70(6)
4.4 Risk assessment
76(4)
4.5 Control of substances hazardous to health
80(3)
4.6 Fire regulations
83(2)
4.7 Personal protective equipment
85(2)
4.8 Reporting of Injuries, Diseases and Dangerous Occurrences Regulations
87(1)
4.9 Transport regulations
88(1)
4.10 Personal health and safety
89(3)
4.11 Universal or standard precautions
92(1)
Summary
92(1)
Further reading
93(1)
Questions
93(2)
5 Statistics and handling data 95(30)
Andrew Blann
Introduction
95(1)
5.1 Types of information and how to describe them
96(9)
5.2 Analysis and interpretation of data
105(15)
5.3 Statistical packages
120(2)
Summary
122(1)
Further truthful
123(1)
Questions
123(2)
6 Preparing and measuring reagents 125(26)
Ian Graham
Introduction
125(1)
6.1 Balances and weighing
126(5)
6.2 Volume measurements and delivery
131(4)
6.3 Pipettors
135(6)
6.4 Preparing reagents: concentrations and dilutions
141(7)
Summary
148(1)
Further reading
149(1)
Questions
149(2)
7 Samples and sample collection 151(28)
Joyce Overfield
Introduction
151(1)
7.1 Blood
152(9)
7.2 Urine
161(2)
7.3 Fluids other than blood and urine
163(4)
7.4 Cytopathology and histopathology samples
167(2)
7.5 Samples for microbiology testing
169(7)
Summary
176(1)
Further reading
176(1)
Questions
177(2)
8 Microscopy 179(32)
Tony Sims
Introduction
179(2)
8.1 Microscopy and image formation
181(6)
8.2 Components of a microscope
187(5)
8.3 Magnification
192(1)
8.4 Dark field microscopy
192(1)
8.5 Phase contrast microscopy
193(2)
8.6 Polarization microscopy
195(1)
8.7 Fluorescence microscopy
195(3)
8.8 Confocal microscopy
198(1)
8.9 Inverted microscopes
199(1)
8.10 Electron microscopy
200(9)
Summary
209(1)
Further reading
209(1)
Questions
209(2)
9 Electrochemistry 211(25)
Peter Robinson
Introduction
211(1)
9.1 Basic concepts and definitions
212(1)
9.2 Principles of electrochemical techniques
213(2)
9.3 Potentiometric techniques
215(10)
9.4 Voltammetric techniques
225(3)
9.5 Biosensors
228(5)
Summary
233(1)
Further reading
233(1)
Questions
234(2)
10 Radioactivity and radiation 236(21)
John Gaffney
Mark Slevin
Introduction
236(1)
10.1 Atomic structure
236(2)
10.2 Types of radioactive decay
238(3)
10.3 Rate of radioactive decay
241(1)
10.4 Units associated with radioactivity
242(1)
10.5 Interaction of radiation with matter
243(1)
10.6 Detection and measurement of radioactivity
244(3)
10.7 Safety precautions when using radioisotopes
247(2)
10.8 Clinical applications of radioactivity
249(5)
Summary
254(1)
Further Reading
254(1)
Questions
255(2)
11 Spectroscopy 257(33)
Qiuyu Wang
Helen Montgomery
Nessar Ahmed
Chris Smith
Introduction
257(1)
11.1 Radiation and the electromagnetic spectrum
257(2)
11.2 Interactions between radiation and matter
259(1)
11.3 Radiation, particles, and quanta
260(1)
11.4 Absorbance
261(5)
11.5 Absorption spectra
266(6)
11.6 Light scattering methods
272(1)
11.7 Fluorescence and fluorimetry
273(1)
11.8 Nuclear magnetic resonance spectroscopy
274(5)
11.9 Mass spectrometry
279(8)
Summary
287(1)
Further reading
288(1)
Questions
288(2)
12 Centrifugation 290(21)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
290(1)
12.1 Basics of centrifugation theory
291(4)
12.2 Types of centrifuges
295(3)
12.3 Tubes and rotors
298(1)
12.4 Separation methods using centrifuges
299(4)
12.5 Safety and centrifuges
303(2)
12.6 Examples of clinical centrifugation
305(3)
Summary
308(1)
Further Reading
308(1)
Questions
309(2)
13 Chromatography 311(30)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
311(1)
13.1 Partition or distribution coefficient
312(2)
13.2 Planar chromatography
314(5)
13.3 Column chromatography
319(10)
13.4 High performance liquid chromatography
329(5)
13.5 Gas-liquid chromatography
334(3)
Summary
337(1)
Further Reading
338(1)
Questions
338(3)
14 Electrophoresis 341(36)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
341(1)
14.1 Principles of electrophoresis
341(2)
14.2 Factors affecting electrophoretic separations
343(2)
14.3 Detection of separated components
345(3)
14.4 Paper or cellulose electrophoresis
348(1)
14.5 Cellulose acetate electrophoresis
349(2)
14.6 Starch electrophoresis
351(1)
14.7 Polyacrylamide gel electrophoresis
352(10)
14.8 Agarose gel electrophoresis
362(5)
14.9 Capillary electrophoresis
367(3)
14.10 Isoelectric focusing
370(2)
14.11 Two-dimensional electrophoresis
372(2)
Summary
374(1)
Further Reading
374(1)
Questions
375(2)
15 Immunological techniques 377(35)
Christine Yates
Introduction
377(1)
15.1 Outline of the immune system
378(2)
15.2 Synthesis of antibodies in vivo
380(2)
15.3 Production of polyclonal and monoclonal antibodies in vitro
382(3)
15.4 Antigen-antibody interactions
385(4)
15.5 Immunological techniques used in clinical laboratories
389(20)
Summary
409(1)
Further reading
410(1)
Questions
411(1)
16 Molecular biology techniques 412(49)
Qiuyu Wang
Nessar Ahmed
Chris Smith
Introduction
412(1)
16.1 Structure and properties of macromolecules
413(9)
16.2 Molecular biology-based techniques
422(1)
16.3 Isolation of nucleic acids
422(6)
16.4 Hydrolysis of nucleic acids and restriction endonudeases
428(6)
16.5 Gel electrophoresis
434(1)
16.6 DNA sequencing
435(4)
16.7 Blotting techniques
439(7)
16.8 Enzyme-linked immunosorbent assays and fluorescence in situ hybridization
446(3)
16.9 DNA cloning
449(8)
16.10 DNA microarrays
457(1)
Summary
458(1)
Further reading
459(1)
Questions
460(1)
17 Laboratory automation 461(17)
Tim James
Introduction
461(1)
17.1 Benefits of automation
462(3)
17.2 Collection of suitable samples and delivery to the laboratory
465(2)
17.3 Specimen reception considerations in the core automated laboratory
467(3)
17.4 Tracked automation systems and the core automated laboratory
470(3)
17.5 Automation in wider laboratory settings
473(2)
Summary
475(1)
Further reading
476(1)
Questions
476(2)
18 Point of care testing 478(27)
Jan Still
Lynda Petley
Introduction
478(1)
18.1 Standards and guidelines
479(1)
18.2 Advantages and limitations of point of care testing
480(4)
18.3 POCT committee and POCT policy
484(4)
18.4 Procurement
488(1)
18.5 Evaluating a POCT device
489(3)
18.6 Use of POCT devices
492(2)
18.7 Connectivity
494(1)
18.8 Training for POCT
495(1)
18.9 Quality and POCT
496(2)
18.10 Audit
498(1)
18.11 Problems, incidents, and litigation
499(3)
Summary
502(1)
Further reading
502(1)
Questions
503(2)
19 Quality assurance and management 505(21)
Elaine Moore
Introduction
505(1)
19.1 Quality control
506(2)
19.2 Quality management
508(4)
19.3 Documentation in the clinical laboratory
512(1)
19.4 Standards and regulatory requirements in the medical laboratory
513(4)
19.5 Tools and techniques for continual improvement
517(7)
Summary
524(1)
Further reading
524(1)
Questions
525(1)
20 Personal development 526(27)
Georgina Lavender
Introduction
526(2)
20.1 Continuing professional development
528(1)
20.2 Opportunities for personal development in the workplace
529(3)
20.3 Raining and development
532(2)
20.4 Professional bodies and personal development
534(6)
20.5 Commercial concerns and personal development
540(1)
20.6 Opportunities for self-guided personal development
541(1)
20.7 Reflective learning
541(1)
20.8 Study Skills
542(5)
20.9 Evidence supporting personal development
547(2)
20.10 Performance appraisal and personal development plans
549(1)
Summary
550(1)
Further reading
551(1)
Questions
551(2)
Glossary 553(10)
Index 563
Hedley Glencross is the Professional Practice Liaison Officer at the Institute of Biomedical Science. Hedley gained 28 years practice in the NHS before taking on his present role with the Institute. He was a previous Institute Council member (1995-2005), part-time lecturer to BSc, MSc and FIBMS courses (1990-2005), IBMS Specialist Advisor for cytology (2001-2005), and examiner for the Institute and NHS Cervical Screening Programme (1996-2005). Dr Nessar Ahmed is Senior Lecturer in Clinical Biochemistry at the Manchester Metropolitan University where his research examines the role of protein glycation in diabetes. His teaching includes clinical biochemistry, endocrinology, and analytical science. Dr Qiuyu Wang is a lecturer in nutritional physiology at Manchester Metropolitan University. She teaches many aspects of physiology and molecular and cellular biology. She has major research interests in cancer biology and the activities of stem cells.