Notes on contributors |
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ix | |
Preface |
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xi | |
Acknowledgements |
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xiii | |
PART I Biological recognition |
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1 | (46) |
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Principles of antigen-antibody recognition |
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3 | (16) |
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3 | (1) |
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Structure of immunoglobulins |
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4 | (1) |
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5 | (1) |
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6 | (3) |
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Antigen-antibody interactions |
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9 | (4) |
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The specificity of immunological reagents |
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13 | (1) |
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Structure-function relationships in immunochemistry |
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14 | (1) |
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15 | (4) |
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Protein-protein interactions |
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19 | (14) |
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Characterization of protein-protein interfaces |
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19 | (4) |
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Double mutant cycle analysis of a protein-protein interface |
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23 | (2) |
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Mutational induced structural rearrangement in protein interfaces |
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25 | (1) |
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Kinetic pathway of protein-protein association |
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25 | (1) |
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The mechanism of association of a protein complex |
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26 | (1) |
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Measuring protein-protein interactions in homogeneous and heterogeneous phase |
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27 | (2) |
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29 | (1) |
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29 | (1) |
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30 | (3) |
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33 | (14) |
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36 | (1) |
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37 | (2) |
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39 | (3) |
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42 | (5) |
PART II Immobilisation of biomolecules |
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47 | (74) |
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Immobilisation chemistry of biological recognition molecules |
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49 | (38) |
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49 | (1) |
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Creation of the molecular recognition interface on the transducer |
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49 | (1) |
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50 | (10) |
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Adsorption to the transducer surface |
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60 | (1) |
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60 | (4) |
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Covalent coupling chemistry |
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64 | (10) |
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Techniques giving some control over the orientation of the immobilised antibody |
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74 | (4) |
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Spatial control of surface immobilisation |
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78 | (5) |
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83 | (1) |
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83 | (4) |
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Binding isotherms and kinetics of immobilized biological systems |
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87 | (34) |
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87 | (2) |
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The determination of binding constants and of kinetic rate constants |
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89 | (29) |
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118 | (1) |
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118 | (3) |
PART III Transducer technology |
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121 | (118) |
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123 | (53) |
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123 | (1) |
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Optical transducers: general considerations |
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124 | (9) |
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133 | (25) |
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158 | (12) |
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170 | (2) |
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172 | (1) |
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172 | (4) |
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176 | (31) |
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176 | (1) |
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176 | (4) |
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180 | (3) |
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Acoustic wave sensors for studying biomolecular interactions |
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183 | (17) |
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Comparison of acoustic sensors |
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200 | (2) |
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202 | (1) |
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202 | (1) |
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202 | (5) |
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Immunoassays using enzymatic amplification electrodes |
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207 | (32) |
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207 | (1) |
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Coupling of immunoassays with enzymatic recycling electrodes |
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207 | (23) |
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230 | (2) |
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232 | (1) |
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232 | (7) |
PART IV Applications |
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239 | (78) |
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Surface plasmon resonance: development and use of BIACORE instruments for biomolecular interaction analysis |
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241 | (28) |
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241 | (3) |
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244 | (1) |
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SPR-refractometer instrumental configurations |
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245 | (11) |
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256 | (3) |
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Biomolecular interaction analysis-BIA |
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259 | (4) |
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263 | (2) |
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265 | (4) |
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IAsys: the resonant mirror biosensor |
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269 | (22) |
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269 | (1) |
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Modus operandi--light and surfaces |
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269 | (2) |
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271 | (3) |
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274 | (13) |
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287 | (1) |
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287 | (4) |
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Commercial quartz crystal microbalances: theory and applications |
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291 | (13) |
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291 | (1) |
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Quartz crystal microbalance--theory |
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291 | (4) |
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295 | (1) |
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295 | (4) |
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Conclusion and future directions |
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299 | (1) |
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300 | (4) |
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The quartz crystal microbalance with dissipation monitoring (QCM-D) |
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304 | (13) |
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304 | (1) |
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The QCM beyond the Sauerbrey regime |
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305 | (2) |
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307 | (1) |
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307 | (2) |
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Example 2: Mussel adhesive protein |
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309 | (3) |
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312 | (1) |
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313 | (1) |
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314 | (3) |
Index |
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317 | |