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E-grāmata: Biosafety Assessment of Probiotic Potential

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Biosafety Assessment of Probiotic PotentialPalielināt
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This volume provides methods on procedures for assessing the biosafety aspects of probiotics. Chapters are divided into five parts detailing in vitro biosafety assessment, biogenic amine production, D-lactic acid production, toxin production, production of various enzymes, determination of toxicity, mutagenicity, virulence genes, capsule formation, hemolytic activity, DNAse activity, bile salt deconjugation, antibiotic resistance, antibiotic resistance gene transfer, mucin degradation, platelet aggregation, and in vivo biosafety assessment of probiotics including determination of  infectivity, reproductive and developmental toxicity, and evaluation of immunological parameters in animal models.

Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  


Determination of biogenic Amine Production.- Determination of
Gelatinases,Glycosidases, and Enolase production.- Determination of
-Glucuronidase Production.- Determination of Nitroreductase Production.-
Determination of Azoreductase production.- Determination of Hemolytic
activity.- Determination of DNAse Activity.- Determination of Bile Salts
Deconjugation.- Determination of D-lactic Acid Production.- Determination of
Antibiotic Resistance.- Determination of Antibiotic Resistance Gene
Transfer.- Determination of Toxin Production.- Detection of Toxin Genes by
PCR Based Methods.- Determination of Presence of Plasmids and Virulence and
Structural Genes.- Determination of Toxicity through Cytotoxicity Assays.- In
vitro Evaluation of the Nitric Oxide Pathway.- Assessment of Capsule
Formation.- Assessment of Platelet aggregation.- Assessment of Fibrinogen and
Fibronectin Binding Activity.- Assessment of ProbioticsAdhesion to Mammalian
Cells.- Assessment of Mutagenicity.- Assessment of Induction and Destruction
of Thrombi.- Assessment of degradation of mucin.- Evaluation of General
Health Status of the Animals during the in-Life Phase.- Assessment of
Bacterial Translocation Through Blood Cultures.- Determination of Splenic
weight index and Weight-to-length ratio.- Determination of Total liver
glutathione and plasma malondialdehyde concentrations.- Determination of
Serum Lactate and Fecal Calprotectin for Assessing The Intestinal
Inflammation.- In vivo Evaluation of Adhesion properties of
Probiotics.- Determination of Streptomyces Probiotics oral administration in
broiler chicken.- Determination of infectivity of probiotics using animal
models.- Determination of Infectivity of Probiotics using Immunosuppressed
Hosts.- Assessment of Reproductive toxicity.- Assessment of Developmental
toxicity in Zebrafish model.- Assessment of Inflammation in Animal Models
(Macroscopic Or Histological Inflammation In The Ileum Or In The
Colon).- Assessment of inflammation in animal models (Quantification of TNFA,
IFNG, IL4, and IL10 mRNAs by Real-time PCR).- Assessment of inflammation in
animal models (Quantification of TNF-, IFN-, IL-4, & IL-10 proteins by
ELISA).- Detection of Myeloperoxidase activity by Enzyme Linked Immunosorbent
Assay.- Assessment of Bacterial Translocation Through Mesenteric Lymph Nodes
[ MLN] and Spleen Cultures.
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