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E-grāmata: Biotechnology and Biology of Trichoderma

Edited by (Vienna University of Technology, Austria), Edited by (National Laboratory of Genomics for Biodiversity, Mexico), Edited by (Banaras Hindu University, India), Edited by , Edited by (Plants), Edited by (Department of Chemistry and Biotechnology, Tallinn University of Technology, Estonia)
  • Formāts: EPUB+DRM
  • Izdošanas datums: 17-Feb-2014
  • Izdevniecība: Elsevier Science Ltd
  • Valoda: eng
  • ISBN-13: 9780444595942
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  • Formāts: EPUB+DRM
  • Izdošanas datums: 17-Feb-2014
  • Izdevniecība: Elsevier Science Ltd
  • Valoda: eng
  • ISBN-13: 9780444595942
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Biotechnology and Biology of Trichoderma serves as a comprehensive reference on the chemistry and biochemistry of one of the most important microbial agents, Trichoderma, and its use in an increased number of industrial bioprocesses for the synthesis of many biochemicals such as pharmaceuticals and biofuels. This book provides individuals working in the field of Trichoderma, especially biochemical engineers, biochemists and biotechnologists, important information on how these valuable fungi can contribute to the production of a wide range of products of commercial and ecological interest.

  • Provides a detailed and comprehensive coverage of the chemistry, biochemistry and biotechnology of Trichoderma, fungi present in soil and plants
  • Includes most important current and potential applications of Trichoderma in bioengineering, bioprocess technology including bioenergy & biofuels, biopharmaceuticals, secondary metabolites and protein engineering
  • Includes the most recent research advancements made on Trichoderma applications in plant biotechnology and ecology and environment

Papildus informācija

A comprehensive reference on the chemistry/biochemistry of one of the most important microbial agents, trichoderma, and its use in an increased number of industrial bioprocesses for the synthesis of many biochemicals such as pharmaceuticals and biofuels.
Preface xi
Foreword xiii
List of Contributors
xv
A BIOLOGY AND BIODIVERSITY
1 Biodiversity of the Genus Hypocrea/Trichoderma in Different Habitats
Laszlo Kredics
Lorant Hatvani
Shahram Naeimi
Peter Kormoczi
Laszlo Manczinger
Csaba Vagvolgyi
Irina Druzhinina
Introduction
3(1)
Methodology of Studying Trichoderma Biodiversity
3(2)
Trichoderma Diversity in Different Habitats
5(13)
Conclusions
18(1)
Acknowledgments
18(1)
References
18(7)
2 Ecophysiology of Trichoderma in Genomic Perspective
Lea Atanasova
Trichoderma in Its Ecological Niche
25(2)
From Diversity to Genomics
27(1)
Mycotrophy of Trichoderma
28(2)
Saprotrophy of Trichoderma on Dead Wood
30(1)
Trichoderma Growth in Soil
31(1)
Rhizosphere Competence of Trichoderma
32(1)
Trichoderma versus Mycorrhizae
32(1)
Trichoderma + Bacteria = ?
33(1)
Facultative Endophytism of Trichoderma
33(1)
Animal Nourishment of Trichoderma
34(1)
Most of the Famous Trichoderma Species are Environmental Opportunists
34(1)
Versatile Carbon Utilization Patterns Reflect Ecological Specialization of Trichoderma spp
35(2)
Acknowledgments
37(1)
References
37(4)
3 DNA Barcode for Species Identification in Trichoderma
Lorant Hatvani
Csaba Vagvolgyi
Laszlo Kredics
Irina Druzhinina
Introduction
41(1)
The Tools
42(1)
Application of DNA Barcoding in Species-Level Identification of Trichoderma
43(1)
Taxonomic Studies
43(2)
Biodiversity Studies
45(2)
Identification of Industrial Trichoderma Strains
47(1)
Identification of Biocontrol Trichoderma Strains
48(2)
Identification of Trichoderma Isolates with Clinical Relevance
50(1)
Identification of Mushroom Pathogenic Trichoderma Strains
51(1)
Conclusions
51(1)
Acknowledgments
52(1)
References
52(5)
4 Understanding the Diversity and Versatility of Trichoderma by Next-Generation Sequencing
Christin Zachow
Gabriele Berg
Introduction
57(1)
Access to Fungal and Trichoderma Diversity---Taxonomic Profiling
58(4)
Plants Life under Control of Trichoderma---Functional Profiling
62(1)
Conclusion
63(1)
Acknowledgments
63(1)
References
63(4)
5 Molecular Evolution of Trichoderma Chitinases
Verena Seidl-Seiboth
Katarina Ihrmark
Irina Druzhinina
Magnus Karlsson
Introduction
67(1)
Phylogeny and Evolution of the GH Family 18 Gene Family in Trichoderma
68(1)
Subgroup A Chitinases
69(2)
Subgroup B Chitinases
71(3)
Subgroup C Chitinases
74(3)
Conclusions
77(1)
Acknowledgments
77(1)
References
77(4)
B SECRETION AND PROTEIN PRODUCTION
6 Protein Production---Quality Control and Secretion Stress Responses in Trichoderma reesei
M. Saloheimo
T. Pakula
N. Aro
J.J. Joensuu
Introduction---Milestones of Trichoderma reesei
81(1)
Protein Secretome of T. reesei
82(2)
ER Quality Control and Secretion Stress Responses
84(2)
Conclusion
86(1)
References
86(3)
7 Heterologous Expression of Proteins in Trichoderma
Helena Nevalainen
Robyn Peterson
Introduction
89(3)
Promoter Options
92(1)
Fusion Partners
93(1)
Extracellular Proteases
94(1)
Secretion Stress in the Frame
95(2)
Mass Production of Heterologous Protein by Fermentation
97(1)
N-glycosylation of Heterologous Proteins Produced in T. reesei
97(1)
Conclusions
98(1)
Acknowledgments
99(1)
References
99(4)
8 Trichoderma Secretome: An Overview
Sunil S. Adav
Siu Kwan Sze
Introduction
103(2)
Proteomic Analysis of Secretory Proteins
105(1)
Extraction of Extracellular Proteins for Proteomic Analysis
106(1)
Extracellular Protein Secretion by T. reesei
107(1)
Polysaccharide Degradation Machinery of T. reesei
108(1)
New Candidates in Cellulose Degradation
109(1)
Hemicellulose Hydrolyzing Enzymes
110(1)
Lignin Degradation by T. reesei
111(1)
Industrial Applications of T. reesei Cellulolytic Enzymes
111(1)
Conclusion
112(1)
References
112(3)
9 The Secretory Pathway in the Filamentous Fungus Trichoderma
Marco J. Hernandez-Chavez
Roberto J. Gonzalez-Hernandez
Jose E. Trujillo-Esquivel
Arturo Hernandez-Cervantes
Hector M. Mora-Montes
Introduction
115(1)
Translocation
115(1)
Cotranslational Translocation
116(1)
Post Translational Translocation
116(1)
Protein Modifications in the ER
116(2)
Vesicle Transport from ER to Golgi Complex and Trafficking within the Golgi Cisternae
118(1)
Transport after Trafficking within the Golgi Complex
119(1)
Secreted Proteins in Trichoderma
119(1)
Concluding Remarks
120(1)
Acknowledgments
120(1)
References
120(5)
C SECONDARY METABOLISM
10 Secondary Metabolism and Antimicrobial Metabolites of Trichoderma
Rosa Hermosa
Rosa Elena Cardoza
Maria Belen Rubio
Santiago Gutierrez
Enrique Monte
Introduction
125(1)
Peptaibols
126(3)
Diketopiperazine-Like Compounds
129(1)
Polyketides
129(1)
Pyrones
130(1)
Terpenes
131(2)
Concluding Remarks and Future Directions
133(1)
Acknowledgments
134(1)
References
134(5)
11 Recent Advancements on the Role and Analysis of Volatile Compounds (VOCs) from Trichoderma
Shafiquzzaman Siddiquee
Introduction
139(1)
Detection Techniques of VOCs
140(2)
Types of Volatiles Compounds
142(23)
Application of VOCs in Agriculture
165(3)
Conclusion
168(1)
References
168(11)
D TOOLS
12 Molecular Tools for Strain Improvement of Trichoderma spp
Robert Bischof
Bernhard Seiboth
Introduction
179(1)
Genetic Transformation Techniques
180(1)
Auxotrophic and Dominant Selection Markers
181(1)
Marker Recycling Strategies and Marker Free Strains
182(1)
Advanced Methods for Gene Targeting
183(1)
RNA Mediated Gene Silencing
184(1)
Promoters for Recombinant Protein Expression and Targeting
185(3)
Concluding Remarks
188(1)
References
188(5)
13 Genetic Transformation and Engineering of Trichoderma reesei for Enhanced Enzyme Production
Anli Ceng
Introduction
193(1)
Engineering Cellulase and Hemicellulase Regulation
194(1)
Homologous and Heterologous Gene Expression and Gene Disruption
195(1)
Protein Engineering
196(1)
Engineering Promoters
197(1)
Conclusion
198(1)
References
198(3)
14 Applications of RNA Interference for Enhanced Cellulase Production in Trichoderma
Shaowen Wang
Gang Liu
Introduction
201(1)
RNA Interference in Fungus
202(1)
Transcriptional Regulation of Cellulase Gene Expression
203(1)
Application of Gene Downregulation Strategy for Enhanced Cellulase Production
204(4)
Combination of RNAi and Overexpression of the Regulating Genes
208(3)
Conclusions and Prospects
211(1)
References
211(4)
15 RNAi-Mediated Gene Silencing in Trichoderma: Principles and Applications
Xiaoyun Su
Lina Qin
Zhiyang Dong
Introduction
215(1)
Molecular Mechanisms
216(2)
Advantages and Disadvantages of Using RNAi-Mediated Gene Silencing as a Genetic Manipulation Tool in Filamentous Fungi
218(2)
Strategies of Applying RNAi for Gene Silencing in Trichoderma and Other Filamentous Fungi
220(3)
Conclusions
223(1)
References
224(5)
E CELLULASES
16 Cellulase Systems in Trichoderma: An Overview
Luis H.F. Do Vale
Edivaldo X.E. Filho
Robert N.G. Miller
Carlos A.O. Ricart
Marcelo V. De Sousa
Introduction
229(1)
Degradation of Cellulose by Cellulase Systems
230(2)
History of the Trichoderma Cellulase Research
232(1)
Structural and Functional Diversity of Trichoderma Cellulases
232(8)
Cellulase Systems and Complexes
240(1)
Acknowledgments
241(1)
References
241(4)
17 Use of Cellulases from Trichoderma reesei in the Twenty-First Century---Part I: Current Industrial Uses and Future Applications in the Production of Second Ethanol Generation
Nicolas Lopes Ferreira
Antoine Margeot
Senta Blanquet
Jean-Guy Berrin
Overview of the Global Enzyme Market
245(1)
Industrial Cellulases
246(3)
Current Applications
249(4)
Perspectives
253(1)
Application of Trichoderma Cellulases in the Bioethanol Industry
253(5)
References
258(5)
18 Use of Cellulases from Trichoderma reesei in the Twenty-First Century---Part II: Optimization of Cellulolytic Cocktails for Saccharification of Lignocellulosic Feedstocks
Jean-Guy Berrin
Isabelle Herpoel-Gimbert
Nicolas Lopes Ferreira
Antoine Margeot
Senta Heiss-Blanquet
Genetics of Industrial Trichoderma reesei Strains
263(1)
The T. reesei Enzyme Cocktail
264(2)
Hydrolysis of Cellulose
266(1)
Limitations in Lignocellulose Hydrolysis
267(2)
Improvement of Enzyme Cocktails by Optimization of Enzyme Ratios
269(1)
Improvement by Supplementation of T. reesei Enzyme Cocktails
270(5)
Adapting Cellulose Cocktails to Process Conditions
275(1)
Conclusions and Perspectives
275(1)
References
275(6)
19 Beta-Glucosidase from Trichoderma to Improve the Activity of Cellulase Cocktails
Warawut Chulalaksananukul
Introduction
281(1)
Cellulase Classification
282(1)
Trichoderma reesei Cellulases
282(2)
Trichoderma reesei BGLs
284(1)
BGLs from Aspergillus oryzae
284(2)
Synergism between Cellulases
286(1)
Heterologous Expression of Cellulases
286(1)
Yarrowia Lipolytica Expression Platforms
286(1)
Pichia pastoris Expression Platforms
287(1)
β-Glucosidase from Trichoderma to Improve the Activity of Cellulase Cocktails
287(1)
Acknowledgments
288(1)
References
288(3)
20 Regulation of Glycoside Hydrolase Expression in Trichoderma
Hoda Bazafkan
Doris Tisch
Monika Schmoll
Introduction
291(1)
Regulation by Environmental Parameters
292(5)
Regulatory Mechanisms
297(5)
Physiological Responses
302(1)
References
303(6)
21 Trichoderma Proteins with Disruption Activity on Cellulosic Substrates
Christian Derntl
Astrid R. Mach-Aigner
Robert L. Mach
Structure and Occurrence of Cellulose in Nature
309(1)
General Aspects of Cellulose Degradation
310(1)
Cellulose Degradation by T. reesei
311(3)
Cellulolytic Enzymes in Other Trichoderma Species
314(1)
Acknowledgments
314(1)
References
314(5)
22 Molecular Mechanism of Cellulase Production Systems in Trichoderma
Katoch Meenu
Gurpreet Singh
R. A. Vishwakarma
Introduction
319(1)
Cellulase System of T. reesei
319(1)
Induction Mechanism of Cellulase Production
320(1)
Promoter Involved in Cellulase Production
320(1)
Molecular Mechanism of Cellulase Production
320(1)
Approaches for Refining the Cellulases Production System in T. reesei
321(1)
References
322(3)
23 Trichoderma in Bioenergy Research: An Overview
Vijai K. Gupta
Anthonia O'Donovan
Maria G. Tuohy
Gauri Dutt Sharma
Introduction
325(1)
Fungal Enzyme Systems and Trichoderma Technology
326(1)
Industrial Applications of Trichoderma
327(1)
Trichoderma Enzyme Systems in Bioenergy Research
328(4)
Conclusion
332(1)
References
332(7)
F INDUSTRIAL APPLICATIONS
24 Trichoderma Enzymes for Food Industries
Adinarayana Kunamneni
Francisco J. Plou
Antonio Ballesteros
Introduction
339(1)
Fungus of Industrial Interest
340(1)
Trichoderma Enzymes for Industries
340(1)
Xylanases
341(1)
Cellulases
341(1)
Other Enzymes
342(1)
Food Industry
342(1)
Perspectives for Biotechnological Production of Enzymes by Trichoderma
343(1)
References
343(2)
25 Trichoderma: A Dual Function Fungi and Their Use in the Wine and Beer Industries
Carlos Roberto Felix
Eliane Ferreira Noronha
Robert N. G. Miller
Introduction
345(2)
Application in the Wine and Beer Industries
347(1)
Acknowledgments
348(1)
References
348(3)
26 Trichoderma Enzymes for Textile Industries
Terhi Puranen
Marika Alapuranen
Jari Vehmaanpera
Substrate
351(1)
Enzymes
352(1)
Textile Processes
353(2)
Trichoderma Enzymes in Textile Finishing Processes
355(2)
Trichoderma as a Production Host for Textile Enzymes
357(2)
Future Trends
359(1)
Acknowledgments
359(1)
References
359(4)
27 Metabolic Diversity of Trichoderma
Roberto Nascimento Silva
Andrei Stecca Steindorff
Valdirene Neves Monteiro
Introduction
363(1)
Global Metabolism
364(2)
Carbohydrate Metabolism and Glycoside Hydrolases
366(2)
Energy Metabolism
368(1)
Secondary Metabolism
369(3)
Metabolism and Transporters
372(2)
Acknowledgments
374(1)
References
374(3)
28 Sequence Analysis of Industrially Important Genes from Trichoderma
Ahmed M.A. El-Bondkly
Introduction
377(1)
Gene Sequence Analysis Fundamentals
378(5)
Genome Analysis of Trichoderma
383(1)
Industrially Genes from Trichoderma
384(1)
Sequence Analysis of Industrially Genes from Trichoderma
384(5)
Conclusion
389(1)
References
390(3)
29 Biosynthesis of Silver Nano-Particles by Trichoderma and Its Medical Applications
Khabat Vahabi
Sedigheh Karimi Dorcheh
Introduction
393(2)
SNP Biosynthesis
395(2)
Mechanism
397(2)
Medical Application
399(1)
References
400(5)
30 Role of Trichoderma Species in Bioremediation Process: Biosorption Studies on Hexavalent Chromium
Dhara Shukla
Padma S. Vankar
Introduction
405(2)
Hexavalent Chromium Bioremediation will Be Discussed Here with a Case Study Representing Chromium Biosorption by Trichoderma Species
407(4)
Conclusion
411(1)
References
412(3)
G BIOCONTROL AND PLANT GROWTH PROMOTION
31 Applications of Trichoderma in Plant Growth Promotion
Alison Stewart
Robert Hill
Introduction
415(1)
Trichoderma as a Plant Growth Promoter
416(2)
Consistency of Growth Promotion
418(1)
Commercialization
419(1)
Mechanisms of Growth Promotion
420(5)
Conclusions
425(1)
References
425(4)
32 Molecular Mechanisms of Biocontrol in Trichoderma spp. and Their Applications in Agriculture
Vianey Olmedo Monfil
Sergio Casas-Flores
Introduction
429(1)
Mycoparasitism
430(1)
Morphological Changes
430(1)
Roll of Cell Wall Degrading Enzymes
431(1)
Signal Transduction in Mycoparasitism
432(1)
ROS-Nox-Signal Transduction
433(2)
Antibiosis (Secondary Metabolites Involved in Biocontrol)
435(1)
Pyrones
436(1)
Polyketides
437(1)
Nonribosomal Peptides
437(1)
Mycotoxins Produced by Trichoderma spp
438(1)
Synergism between Enzymes and Antibiotics
439(1)
Competition for Nutrients
439(1)
Plant Growth Promotion by Trichoderma
440(2)
Plant Root Colonization
442(1)
Induction of Systemic Resistance to Plants by Trichoderma spp
443(1)
Signal Transduction Pathways that Mediate Trichoderma-Plant Communication
444(2)
Trichoderma Elicitor of Systemic Resistance in Plants
446(2)
Signal Transduction during Plant--Trichoderma Interaction in Trichoderma
448(1)
Transgenic Plants Expressing Trichoderma Genes
448(1)
Concluding Remarks
449(1)
Acknowledgments
449(1)
References
449(6)
33 Genome-Wide Approaches toward Understanding Mycotrophic Trichoderma Species
Alfredo Herrera-Estrella
Introduction
455(2)
Lessons from the Genome Sequence
457(1)
Transcriptome Analyses
458(1)
The Functional Genomics View of Mycoparasitism
458(1)
High-Throughput Analysis of the Trichoderma-Plant Interaction
459(2)
Future Directions
461(1)
Concluding Remarks
462(1)
Acknowledgments
462(1)
References
462(3)
34 Insights into Signaling Pathways of Antagonistic Trichoderma Species
Susanne Zeilinger
Sabine Gruber
Introduction
465(1)
G Protein Signaling
465(1)
Effector Pathways of G Protein Signaling in Fungi
466(1)
Signaling Pathways and Characterized Components in Trichoderma Species
467(2)
Signal Transduction Components and Pathways Affecting Vegetative Growth and Conidiation
469(2)
The Role of Signaling in Trichoderma Mycoparasitism and Biocontrol
471(3)
Conclusions
474(1)
Acknowledgments
474(1)
References
474(3)
35 Enhanced Resistance of Plants to Disease Using Trichoderma spp
M.G.B. Saldajeno
H.A. Naznin
M.M. Elsharkawy
M. Shimizu
M. Hyakumachi
Introduction
477(1)
Induced Disease Resistance in Plants
478(3)
Induced Resistance by Trichoderma spp
481(1)
Signaling Pathways of Trichoderma-Induced Resistance
482(1)
Trichoderma spp.-Secreted Elicitors of Plant Resistance
483(2)
Engineering Plants for Disease Resistance Using Trichoderma Genes
485(1)
Combination of Trichoderma with Other Beneficial Microorganisms
486(1)
Other Effects of Trichoderma spp. Inoculation to the Plant
487(1)
Conclusion
487(1)
References
488(7)
36 Enhanced Plant Immunity Using Trichoderma
Hexon Angel Contreras-Cornejo
Lourdes Macias-Rodriguez
Jesus Salvador Lopez-Bucio
Jose Lopez-Bucio
Introduction
495(1)
Mechanisms of Plant Protection by Microbes
495(3)
Trichoderma-Induced Immunity
498(2)
Plant Protection Conferred by Trichoderma
500(1)
Conclusions
501(1)
Acknowledgments
501(1)
References
501(4)
37 Genes from Trichoderma as a Source for Improving Plant Resistance to Fungal Pathogen
Barbara Reithner
Robert L. Mach
Introduction
505(1)
Trichoderma Inducing Resistance in Plants
506(1)
Transgenic Plants Expressing Trichoderma Genes Develop Increased Resistance to Fungal Pathogens
506(2)
Trichoderma Genes Involved in Elicitation of ISR
508(3)
Conclusion
511(1)
Abbreviations
511(1)
Acknowledgments
511(1)
References
511(4)
38 Trichoderma Species as Abiotic Stress Relievers in Plants
Najam W. Zaidi
Manzoor H. Dar
Sudhanshu Singh
U.S. Singh
Introduction
515(1)
Microbes for the Management of Abiotic Stresses
516(1)
Alleviation of Abiotic Stress in Plants by Trichoderma
516(1)
Alleviation of Drought Stress in Plants by Trichoderma
517(1)
Alleviation of Salinity Stress in Plants by Trichoderma
518(1)
Alleviation of Heat Stress in Plants by Trichoderma
519(1)
Trichoderma Genes for Abiotic Stress Tolerance
520(1)
Mechanism of Abiotic Stress Tolerance Using Trichoderma
520(1)
Host Gene: Stress Tolerant Varieties
521(1)
Conclusion
522(1)
References
523(4)
39 Advances in Formulation of Trichoderma for Biocontrol
Christian Joseph
R. Cumagun
Introduction
527(1)
Types of Formulation
528(1)
Microencapsulation
528(1)
Enhancement of Shelf Life and Application Efficiency
528(1)
Compatibility with Other Biological Systems
529(1)
Conclusion and Future Prospects
530(1)
References
530(3)
40 Trichoderma: A Silent Worker of Plant Rhizosphere
Akanksha Singh
Birinchi K. Sarma
Harikesh B. Singh
R. S. Upadhyay
Introduction
533(1)
Diverseness Amongst Trichoderma
534(2)
Trichoderma as Inducer of Plant Defense Response
536(2)
Trichoderma as a Biofertilizer and Plant Growth Promoter
538(1)
Commercialization
538(1)
Trichoderma Genes Responsible for Playing "Big Games"
539(1)
Conclusion
540(1)
Acknowledgments
540(1)
References
540(3)
Index 543
Dr Vijai G. Gupta is an Assistant Professor of Biotechnology at MITS University of India. Currently he is working as Research Scientist at National University of Ireland in Galway. Dr. Guptas present work is focused on the development and optimization of novel Enzyme-based bioconversion systems for biorefining and bioenergy. He has been honored with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Indian Young Scientist Award. He has submitted 33 new fungal nucleotide sequences and deposited 147 fungal strains in International databases. His work with Fusarium spp., Colletotrichum gloeosporioides, Penicillium spp. and Trichoderma spp. is augmented by contributions to biotechnological development, molecular diversity, secondary metabolites and industrial applications Dr. Gupta is the editor-in-chief of the International Journal of Plant Pathology and a regional editorial board member of 8 other respected journals. He is the author of 40 journal articles and 27 book chapters. Gupta has also written and edited books and series from reputed publishers, including CRC Press, Taylor and Francis, USA; Springer, USA; Elsevier, USA; Nova Science Publisher, USA and LAP Lambert Academic Publishing, Germany. Dr. Monika Schmoll received her degree (1999) and Ph. D. (2003) on regulation of cellulase expression and signal transduction in the filamentous fungus Hypocrea jecorina (Trichoderma reesei) at the Vienna University of Technology. Besides gaining postdoctoral experience and building her own group at the Vienna University of Technology, she has been a visiting scientist in the laboratory of Professor N. Louise Glass (Department of Plant and Microbial Biology, University of California, Berkeley, USA), the University of Rome La Sapienza and the University of Szeged, Hungary. She is author of 33 publications and 2 book chapters. Currently, Dr. Schmoll is group leader in the Research Area Molecular Biotechnology at the Vienna University of Technology. The primary research field of Dr. Schmoll is the interconnection between light response, sexual development and cellulase gene expression in Trichoderma reesei. She showed for the first time that cellulase gene expression is modulated by light in T. reesei and could since then elucidate important mechanistic details on the underlying mechanism. Her group discovered the sexual cycle in the biotechnological workhorse Trichoderma, which had previously considered asexual. This work was published in PNAS in 2009 and since then investigation of this phenomenon and its peculiarities in Trichoderma has become an additional focus. Her work with Trichoderma is complemented by contributions to genome annotation of several fungi (Trichoderma spp., Aspergillus nidulans, Postia placenta, Ceriporiopsis subvermispora), especially in the field of signal transduction. Prof. Alfredo Herrera-Estrella grew up in Mexico City and graduated from the National School of Biological Sciences in 1985. He did his graduate research (1986-1990) with Prof. Marc Van Montagu at the State University of Ghent, Belgium, studying the T-DNA transfer process from Agrobacterium tumefaciens to plants. He described for the first time Agrobacterium virulence proteins capable of carrying the T-DNA into the plant cell nucleus, and began to study the mycoparasitic process of the biocontrol agent Trichoderma atroviride. Dr. Herrera-Estrella pioneered the development of molecular tools for the study of a biocontrol agent with the establishment of transformation systems, and cloning of the first mycoparasitism related genes. Such developments opened possibilities for strain improvement. He continued those studies while at the Genetic Engineering Department of the Irapuato Unit of Cinvestav (1991-2004), and began studies towards the elucidation of the mechanisms involved in light perception in Trichoderma. In 2000, he was awarded the prize of the Mexican Academy of Sciences. By 2004, he got involved in the establishment of the National Laboratory of Genomics for Biodiversity. Since then he and his group have been involved in Functional Genomics Projects, including the elucidation of the complete maize genome, and the development of advanced techniques to study gene expression by deep sequencing. Dr. Herrera-Estrella has continuing efforts in the elucidation of signaling cascades triggering asexual development in fungi. In particular his group has been using functional genomics approaches for this purpose, and recently has been involved in the study of the role of reactive oxygen species as signal molecules in injury responses in fungi. Professor R. S. Upadhyay (b. November 15, 1955) received his M. Sc. (1976) and Ph. D. (1980) degrees from Banaras Hindu University (BHU), Varanasi, India. Since then he has been actively engaged in research. His main focus on research has been on biological control of plant pathogens, programmed plant cell death in response to pathogens, bioremediation of toxic effluents, induced resistance in plants and their immunization, plant growth promoting microbes, chitinase production from actinomycetes, mycorrhizal technology for reclamation of wastelands, role rhizobacteria in detoxifying phytotoxic effects, development of molecular markers in tracking microbes in environment, effect of biotic and abiotic factors on plants, and molecular basis of plant-microbe interaction specially for Trichoderma spp. & Fusarium spp. His work has been well cited internationally. He has been working at Banaras Hindu University in various positions such as Lecturer, (1984-88), Senior Lecturer, (1988-1991), UGC Research Scientist-B, BHU (1988-1991 on lien from BHU), Reader, Banaras Hindu University (1991-1999, appointed in absentia), Professor, (1999 till date), Student Advisor, Faculty of Science, BHU (2010-11), Coordinator, Environmental Science, BHU (2011 till date) and Dy. Coordinator, Centre of Advanced Study in Botany, BHU (2011, for five years). He has been recipient of six prestigious national fellowships of the Government of India. He is recipient of five national awards in the area of science, two conferred by the Prime Minister of India. In addition he worked in prestigious foreign laboratories as visiting Scientist of The Royal Society, London (1988-89), Research Associate of NIH, U.S.A. (1990-91), and INSA- JSPS Fellowship, Japan (1994-95). He has also visited many other courtiers to participate in International conferences or for delivering invited lectures. Dr. Irina Druzhinina studied biology at the Lomonosovs State University in Moscow, Russia, and at the University of Vienna, where she graduated (PhD) in 2001. Thereafter she became a University Assistant in the research area of C.P. Kubicek at Vienna University of Technology, and habilitated in 2011 in Microbiology”. She is now leader of the working group Microbiology” at the same institute.The scientific work of Irina Druzhinina started with a focus on mycology and molecular evolution, working on species diversity and population differentiation in Trichoderma, where she established an online tool for species identification based on DNA barcodes that is today one of the main resources for this purpose for researchers worldwide. In addition, she worked on such diverse topics as phenotype profiling of industrial fungi, peptaibol biosynthesis in Trichoderma, biodiesel production by marine algae, Trichoderma endophytes, biofungicide development and molecular ecology of Trichoderma. In the last years, her interest expanded to ecological genomics, i.e. the use of genome wide information to study the evolutionary adaptation of Trichoderma in its habitats. She acts as a member of the editorial board for Applied and Environmental Microbiology, and is the chair of the International Subcommission on Taxonomy of Trichoderma and Hypocrea, a member of ICTF/IUMS. She published > 60 papers in peer reviewed international journals and edited a book Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, Department of Biochemistry, School of Natural Sciences, NUI Galway which has developed a strong track record in Glycobiotechnology and Enzyme Biotechnology. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy and her group have developed patented enzyme-based technologies for key bioenergy and biorefinery applications from terrestrial and marine biomass and wastes, including 3rd generation feedstocks. The group also investigates the use of enzymes for the recovery and selective modification of high-value biochemicals and plant carbohydrate-derived bioactives (Glycobioengineering). Dr. Tuohy is a PI in the Energy Research Centre, NUI, Galway and the recently funded national Bioenergy and Biorefinery Competence Centre, is a member of the EU FP7 Biofuels Platform and a national research PhytoNetwork. Dr. Tuohy has been a visiting researcher in RUGhent, Belgium and BSH Institut fur Holzchemie, Hamburg. Dr. Tuohy is author of ~132 research publications, including refereed publications, book chapters, conference papers poster/short communications. She is also a reviewer for international journals and funding agencies and several books as co-editor- Springer Science Publisher, USA; CRC Press, Taylor and Francis, USA; Germany; Nova Science Publisher, USA and Elsevier Press, USA (under Progress) with Dr. V. K. Gupta