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E-grāmata: Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2531
  • Izdošanas datums: 08-Aug-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071624937
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Capillary Electrophoresis-Mass Spectrometry: Methods and ProtocolsPalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2531
  • Izdošanas datums: 08-Aug-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071624937
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This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.
Evaluation of prototype CE-MS interfaces.- Capillary
Electrophoresis-Mass Spectrometry (CE-MS) by Sheath-Flow Nanospray interface
and its use in biopharmaceutical applications.- Protocol for etching
bare-fused silica capillaries for sheathless capillary electrophoresis-mass
spectrometry coupling.- Microfabricated Liquid Junction Capillary
Electrophoresis-Mass Spectrometry Interface.- Successive Multiple
Ionic-Polymer Layer Coatings for intact protein analysis by Capillary Zone
Electrophoresis - Mass Spectrometry Application to Hemoglobin
Analysis.- On-line Immobilized Enzyme Microreactor Capillary Zone
Electrophoresis-Mass Spectrometry for Peptide Mapping.- Bottom up analysis of
proteins by peptide mass fingerprinting with tCITP-CZE-ESI-TOF MS after
tryptic digest.- Top-down proteomics by capillary zone electrophoresis-tandem
mass spectrometry for large-scale characterization of proteoforms in complex
samples.- Assessment of macro- and microheterogeneity of monoclonal
antibodies using Capillary Zone Electrophoresis hyphenated with Mass
Spectrometry.- High Sensitivity Glycoproteomic Analysis of Biological Samples
by CZE-ESI-MS.- Capillary Zone Electrophoresis-Electrospray Ionization Tandem
Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate
Oligosaccharides.- In-Source Fragmentation for Identification of Compounds by
CE-ESI-TOF in Human Plasma. L-Proline as case study.- Assessing the energy
status of low numbers of mammalian cells by capillary electrophoresis-mass
spectrometry.- Applications of Capillary Electrophoresis-Mass Spectrometry to
Chiral Analysis.- Improved Sensitivity to Determine Antibiotic Residues in
Chicken Meat by In-Line Solid-Phase Extraction coupled to Capillary
Electrophoresis-Tandem Mass Spectrometry.- CE Coupled to ICP-MS and Single
Particle ICP-MS for Nanoparticle Analysis. 
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