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E-grāmata: Cell-Based Assays Using iPSCs for Drug Development and Testing

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1994
  • Izdošanas datums: 23-May-2019
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493994779
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Cell-Based Assays Using iPSCs for Drug Development and TestingPalielināt
  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1994
  • Izdošanas datums: 23-May-2019
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493994779
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The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.

Cell-Based Assays using Differentiated Human Induced Pluripotent
Cells.- Monolayer Generation of Vascular Endothelial Cells from Human
Pluripotent Stem Cells.- Generating Human iPSC-Derived Astrocytes with
Chemically Defined Medium for In Vitro Disease Modelling.- Human Induced
Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic
Differentiation.- Production of Cardiomyocytes from Human Pluripotent Stem
Cells by Bioreactor Technologies.- Improved Protocol of Cardiac
Differentiation and Maturation of Pluripotent Stem Cells.- Human Pluripotent
Stem Cell Expansion in Stirred Tank Bioreactors.- A Protocol for the One-Step
Differentiation of Human Induced Pluripotent Stem Cells into Mature
Podocytes.- Differentiation of hiPS Cells into Definitive Endoderm for High
Throughput Screening.- LC-MS/MS Based Metabolomics for Cell
Cultures.- Preparation of iPSCs for Targeted Proteomic Analysis.- Measurement
of Bile Acids as a Marker of the Functionality of iPSC-Derived
Hepatocytes.- Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein
Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation.- Seeding
Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates.- High
Content Autophagy Analysis in iPSC-Derived Neurons using
Immuno-Fluorescence.- High Content Analysis of Mitochondrial Function in
iPSC-Derived Neurons.- Evaluating the Effect of Drug Compounds on Cardiac
Spheroids using the Cardiac Cell Outgrowth Assay.- Assay Procedures for
Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well
Microelectrode Arrays.- Multielectrode Array (MEA) Based Detection of
Spontaneous Network Activity in Human iPSC-Derived Cortical
Neurons.- Assessing Neuronal Excitability on a Fluorometric Imaging Plate
Reader (FLIPR) Following a Defined Electrostimulation Paradigm.- Fabrication
of a Microfluidic Cell Culture Device using Photolithographic and Soft
Lithographic Techniques.- Using a Microfluidic Device for Culture and Drug
Toxicity Testing of 3D Cells.- hiPS CellDerived Neurons for High-Throughput
Screening.
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