E-grāmata: Cell Culture for Biochemists

Acknowledgements.
Chapter
1. Introduction. 1.1 Background. 1.2 Some
advantages. 1.3 Applications.1.4 Animal cell biotechnology.
Chapter
2.
Characteristics of cultured cells. 2.1 Types of cells. 2.2 Primary cells and
transformation. 2.3 Growth control. 2.4 Attachment and Spreading. 2.5 Growth
factors. 2.6 Differentiated functions in cell cultures.
Chapter
3. Culture
vessels. 3.1 Design of culture vessels. 3.2 Small scale cultures. 3.3
Intermediate scale cultures. 3.4 Large scale cultures. 3.5 Suspension
cultures. 3.6 Microcarriers. 3.7 Air lift systems. 3.8 Bioreactors.
Chapter
4. Subculturing. 4.1 Dissociation techniques. 4.2 Subculture of a cell
monolayer. 4.3 Subculture of cells growing in suspension. 4.4 Protocol for
setting up microcarrier cultures 4.5 Subculture of cells growing on
microcarriers. 4.6 The growth cycle.
Chapter
5. Cell culture media. 5.1
Introduction. 5.2 Balanced salt solutions. 5.3 Eagle's medium. 5.4 More
complicated media. 5.5 Simple media with unspecified additives. 5.6
Antibiotics. 5.7 Serum. 5.8 Serum-free media. 5.9 Media for culture of insect
cells. 5.10 Media for culture of plant cells.
Chapter
6. Primary cells. 6.1
Introduction. 6.2 Lymphocytes. 6.3 Human skin biopsies. 6.4 Mouse or rat
embryo cultures. 6.5 Chick embryo cells. 6.6 Chick embryo liver cells. 6.7
Rat hepatocytes. 6.8 Primary kidney cells. 6.9 Endothelial cells. 6.10
Mammary epithelial cell cultures. 6.11 Colonic epithelial cells. 6.12 Rat or
chick skeletal muscle cells. 6.13 Mouse macrophage cultures. 6.14 Ascites
cells. 6.15 Dipteran cell culture.
Chapter
7. Techniques. 7.1 Cell cloning
and plating efficiency. 7.2 Cell counting procedures. 7.3 Storage of cells.
7.4 Karyotyping. 7.5 Cell transfection. 7.6 Cell visualisation. 7.7
Sub-cellular fractionation.
Chapter
8. Glassware preparation and
sterilisation techniques. 8.1 General. 8.2 Sterilisation by heat. 8.3
Sterilisation by filtration.
Chapter
9. Contamination. 9.1. Bacterial
contamination. 9.2 Sterility checks. 9.3 Analysis of bacterial contamination.
9.4 Airborne contamination. 9.5 Antibiotics. 9.6 Disposal of contaminated
material. 9.7 Mycoplasmas. 9.8 Testing for mycoplasma 9.9 Elimination of
mycoplasmas. 9.10 Viral contamination.
Chapter
10. The cell cycle. 10.1
Description. 10.2 Mitosis. 10.3 S-phase. 10.4 Control of the cell cycle. 10.5
Distribution of cells around the cycle. 10.6 Growth fraction. 10.7 Cell cycle
analysis.
Chapter
11. Cell synchronisation. 11.1 Intoduction. 11.2 Selection
of mitotic cells. 11.3 Selective killing of cells in particular phases. 11.4
Selection of cells by size. 11.5 Synchronisation by subculture. 11.6 Serum
deprivation. 11.7 Isoleucine starvation. 11.8 Blockade of S-phase. 11.9
Procedure for inducing synchrony at the G1/S interphase. 11.10
Synchronisation in G2. 11.11 Synchronisation in M.
Chapter
12. Use of
radioactive isotopes in cell culture. 12.1 Estimation of rates of DNA
synthesis. 12.2 Estimation of rates of RNA and protein synthesis. 12.3
Autoradiography. 12.4 Labelling with bromodeoxyuridine. 12.5 DNA repair.
Chapter
13. Cell mutants and cell hybrids. 13.1 Auxotrophic mutants. 13.2
Selection of mutants. 13.3 Temperature sensitive mutants. 13.4 Replica
plating of animal cells. 13.5 Somatic cell hybridisation. 13.6 Myeloma
culture and monoclonal antibody production. 13.7 Methods of fusion. 13.8 Cell
communication.
Chapter
14. Viruses. 14.1 Introduction. 14.2 Virus production.
14.3 Virus detection. 14.4 Production and testing of viral vaccines. 14.5
Viral transformation of cells.
Chapter
15. Differentiation in cell cultures.
15.1 Erythroid differentiation of Friend cells. 15.2 Skin and keratinocytes.
15.3 Teratocarcinoma cells. 15.4 Differentiation of muscle cells. 15.5
Differentiation of adipose cells. 15.6 Differentiated hepatocytes.
Chapter
16. Appendices. References. Subject Index.