This volume explores the latest experimental and computer-based methods used to study the field of motility research. The chapters in this book cover topics such as methods to investigate motility and chemotaxis in a range of systems including macrophages and Dictyostelium discoideum; ways in which electric fields impact motility and taxis; recently developed computer-based methods for studying pseudopod-driven locomotion of amoeboid cells; and software tools for kymograph-based analysis of cell contour dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Cell Motility and Chemotaxis: Methods and Protocols is a valuable resource for researchers looking to expand their knowledge of motility-related research across different disciplines.
Chemotaxis Assay of Bone Marrow-Derived Macrophages.- Methods and
Protocol for Single Cell Motility Assays under Topological
Constraints.- Imaging-Based Analysis of Cell-Cell Contact Dependent Migration
in Dictyostelium.- A Mutant of Dictyostelium discoideum, KI-Cell, As a Model
of Collective Cell Migration Independent of Chemotaxis.- Using Multiphoton
Intra-Vital Microscopy to Study Neutrophil Transmigration and Blood-Brain
Barrier Permeability in a Mouse Model of Herpes Simplex Virus
Encephalitis.- An In Vivo Metastasis Model using Genotype-Defined Tumor
Organoids.- Different Morphotypes of Physarum polycephalum as Models for
Chemotaxis and Locomotion.- Flagella and Cell Body Staining of Bacteria with
Fluorescent Dyes.- Re-Purposing Proximity-Dependent Protein Labelling
(BioID2) for Protein Interaction Mapping in E. coli.- Electrotaxis of
Dictyostelium discoideum, Migration in an Electric Field.- Electric
Cell-substrate Impedance Sensing as a Tool to Characterize Wound Healing
Dynamics.- Chemotaxis of Large Multinucleate Cells of Dictyostelium Produced
by Electric-Pulse Induced Fusion.- A Hands-On Guide to AmoePy: A Python-Based
Software Package to Analyze Cell Migration Data.- Pseudopod Tracking and
Statistics during Cell Movement in Buffer and Chemotaxis.- Generation of a
Virtual Cell using a Phase Field Approach to Model Amoeboid Crawling.
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