E-grāmata: Cerebral Cortex Development: Methods and Protocols

Overview: Research on the Genetic Architecture of the Developing
Cerebral Cortex in Norms and Diseases.- In Situ Hybridization of Brain
Slices.- Immunohistochemistry of Brain Tissue.- Two-Photon FRET/FLIM Imaging
of Cerebral Neurons.- Pre- and Post-Embedding Immunoelectron Microscopy to
Analyze the Central Nervous System.- Mouse Brain Tissue Preparation for
Scanning Electron Microscopy.- Simple Method for the Preparation of the
Postsynaptic Density Fraction from Mouse Brain.- Reconstitution of Neuronal
Motor Traffic on Septin-Associated Microtubules.- BioID Analysis of
Actin-Binding Proteins.- Proteomic Analyses of the Mouse Brain.- Generation
of iPS Cells using Sendai Virus Vectors.- Directed Differentiation of Neurons
from Human iPSCs for Modeling Neurological Disorders.- Generating Homogeneous
Brain Organoids from Human iPSCs.- Primary Culture of Dissociated Neurons
from Embryonic Cerebral Cortex.- Immunocytochemistry of Primary Cultured
Cerebral Cortical Neurons.- Brain Sample Preparation after Performing In
Utero Electroporation to Proliferating and Differentiated Cells in the
Developing Mouse Neocortex.- Live Imaging of Migrating Neurons and Glial
Progenitors Visualized by In Utero Electroporation.- Ca2+ Imaging in Immature
Cortical Neurons.- In Vitro Patch-Clamp.- In Vivo Whole-Cell Recording from
the Mouse Brain.- In Vivo Brain Microdialysis.- CT-Scan Data Analysis in
Malformations of Cortical Development.- MRI Data Analysis in Malformations of
Cortical Development.- Genomic Copy Number Analysis using Droplet Digital
PCR: A Simple Method with EvaGreen Single-Color Fluorescent
Design.- Locomotor Assays in Drosophila Larvae and Adult Flies.- Analyses of
Genetic Regulation of the Nervous System in the Nematode Caenorhabditis
elegans.- Analyses of Neural Circuits Governing Behavioral Plasticity in the
Nematode Caenorhabditis elegans.- Social Behavior in Animal Models of Autism
Spectrum Disorder.- Preparation of Healthy Single Neuron or Astrocyte
Suspension from Adult Mouse Brain for RNA-seq.