| Preface |
|
vii | |
| Contributors |
|
xv | |
|
1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice |
|
|
1 | (22) |
|
|
|
|
|
|
|
|
|
2 Role of Heat Shock Factors in Stress-Induced Transcription |
|
|
23 | (12) |
|
|
|
|
|
|
|
|
|
3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter |
|
|
35 | (12) |
|
|
|
|
|
|
|
|
|
4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay |
|
|
47 | (12) |
|
|
|
5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay |
|
|
59 | (16) |
|
Maria A.W.H. van Waarde-Verhagen |
|
|
|
|
6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors |
|
|
75 | (12) |
|
|
|
7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine |
|
|
87 | (10) |
|
|
|
|
|
|
|
8 Primary Colorectal Cells Culture as a Translational Research Model |
|
|
97 | (10) |
|
|
|
Nina Claire Dempsey-Hibbert |
|
|
|
|
|
|
|
|
|
|
9 Cell Death and Survival Assays |
|
|
107 | (22) |
|
|
|
|
|
10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro |
|
|
129 | (10) |
|
|
|
|
|
11 Proteomic Profiling of Hsp90 Inhibitors |
|
|
139 | (24) |
|
|
|
|
|
|
|
|
|
|
|
|
|
12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides |
|
|
163 | (16) |
|
|
|
13 Nucleotide Exchange Factors for Hsp70 Chaperones |
|
|
179 | (10) |
|
|
|
|
|
|
|
14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast |
|
|
189 | (10) |
|
|
|
15 Bacterial Hsp90 ATPase Assays |
|
|
199 | (10) |
|
|
|
|
|
|
|
16 Detecting Posttranslational Modifications of Hsp90 |
|
|
209 | (12) |
|
|
|
|
|
|
|
|
|
17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90) |
|
|
221 | (12) |
|
|
|
|
|
18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond |
|
|
233 | (20) |
|
|
|
|
|
|
|
|
|
19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors |
|
|
253 | (22) |
|
|
|
20 Computational Analysis of the Chaperone Interaction Networks |
|
|
275 | (18) |
|
|
|
|
|
|
|
|
|
|
|
21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer |
|
|
293 | (14) |
|
|
|
|
|
|
|
|
|
|
|
22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody |
|
|
307 | (14) |
|
|
|
|
|
|
|
|
|
|
|
|
|
23 Detection and Analysis of Extracellular Hsp90 (eHsp90) |
|
|
321 | (10) |
|
|
|
Alexander J. Baker-Williams |
|
|
|
|
|
|
24 Molecular Chaperone Receptors |
|
|
331 | (14) |
|
|
|
|
|
|
|
|
|
25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy |
|
|
345 | (14) |
|
|
|
|
|
|
|
26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells |
|
|
359 | (12) |
|
|
|
|
|
|
|
27 Hsp70: A Cancer Target Inside and Outside the Cell |
|
|
371 | (26) |
|
|
|
|
|
|
|
|
|
28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding |
|
|
397 | (26) |
|
|
|
|
|
29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors |
|
|
423 | (20) |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Index |
|
443 | |
Biežāk uzdotie jautājumi par e-grāmatām