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E-grāmata: Characterization of Nanoparticles Intended for Drug Delivery

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2789
  • Izdošanas datums: 20-Mar-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071637869
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Characterization of Nanoparticles Intended for Drug DeliveryPalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2789
  • Izdošanas datums: 20-Mar-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071637869
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This third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in this book are dedicated to physicochemical characterization techniques and cover newer methods such as asymmetric-flow field-flow fractionation, single particle inductively coupled plasma mass spectrometry, and resistive pulse sensing. The next eighteen chapters explore the immunotoxicity of nanomaterials, including microbial contaminants such as endotoxin and beta-glucans, anti-PEG antibodies, autoimmunity, and immunosuppressive properties. The last two chapters talk about new pharmacology protocols, including a new technique to assess drug release and a tissue distribution assay using PEG immunohistochemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Characterization of Nanoparticles Intended for Drug Delivery, Third Edition is a valuable resource that will help both expert and novice researchers further enhance their understanding of this important and developing field.

Advancements in Nanoparticle Characterization.- Nanoparticle Size
Distribution and Stability Assessment Using Asymmetric-Flow Field-Flow
Fractionation.- Assessment of Protein Binding Using Asymmetric-Flow
Field-Flow Fractionation Combined with Multi-Angle Light Scattering and
Dynamic Light Scattering.- Quantitation of Active Pharmaceutical Ingredients
in Polymeric Drug Products Using Elemental Analysis.- Particle Size and
Concentration Measurement Using the Spectradyne nCS1 Instrument.- Measuring
Size and Number Concentration of Metallic Nanoparticles Using spICP-MS.- Ion
Quantitation in Liposomal Products with RP-HPLC and Charged Aerosol
Detection.- A Static Headspace Gas Chromatography Method for Quantitation of
Residual Solvents in Nanoformulations.- Current Considerations and Practical
Solutions for Overcoming Nanoparticle Interference with LAL Assays and
Minimizing Endotoxin Contamination.- Detection of Beta-Glucan Contamination
inNanoparticle Formulations.- Detection of Intracellular Complement
Activation by Nanoparticles in Human T Lymphocytes.- Analysis of
Nanoparticles Potential to Induce Autoimmunity.- Analysis of Nanoparticles
Effects on Drug-Induced Psoriasis.- Detection of Nanoparticle-Mediated Total
Oxidative Stress in T-Cells Using CM-H2DCFDA Dye.- Detection of Induction of
Mitochondrial Oxidative Stress by Nanoparticles in T-Cells Using MitoSOX Red
Dye.- Detection of Nanoparticle-Mediated Change in Mitochondrial Membrane
Potential in T-Cells Using JC-1 Dye.- Detection of Antigen Presentation by
Murine Bone Marrow-Derived Dendritic Cells After Treatment with
Nanoparticles.- Antigen-Specific Stimulation of CD8+ T-Cells by Murine Bone
Marrow-Derived Dendritic Cells After Treatment with Nanoparticles.- Detection
of Pre-Existing Antibodies to Polyethylene Glycol and PEGylated Liposomes in
Human Serum.- In Vitro and In Vivo Methods for Analysis of Nanoparticle
Potential to Induce Delayed-Type Hypersensitivity Reactions.- Analysis of
Nanoparticle Adjuvant Properties.- Methods for Analysis of Nanoparticle
Immunosuppressive Properties.- Evaluation of the Acute Anaphylactoid
Reactogenicity of Nanoparticle-Containing Medicines and Vaccines Using the
Porcine CARPA Model.- Immunophenotyping, Part I: Instrument Calibration and
Reagent Qualification for immunophenotyping Analysis of Human Peripheral
Blood Mononuclear Cell Cultures.- Immunophenotyping, Part II: Analysis of
Nanoparticle Effects on the Composition and Activation Status of Human
Peripheral Blood Mononuclear Cells.- Understanding the Role of Scavenger
Receptor A1 in Nanoparticle Uptake by Murine Macrophages.- Assessment of
Temperature-Dependent Drug Release of Solubilizing Nanoformulations Using the
SITUA.- Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa
Fluor 488 and PEG Immunohistochemistry.
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