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E-grāmata: Chemogenomics: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2706
  • Izdošanas datums: 09-Aug-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071633977
  • Formāts - EPUB+DRM
  • Cena: 142,75 €*
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2706
  • Izdošanas datums: 09-Aug-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071633977

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This volume presents both theoretical guidance and protocols on chemogenomics including chemogenomics library assembly, compound profiling, and phenotypic assays. The chapters in this book cover topics such as the assembly and use of Kinase Chemogenomics; data mining for chemogenomic compound candidates; protocols for protein family-focused assay systems to profile chemogenomic compounds; functional and target engagement assays in cellular settings for broad characterization; and a discussion on phenotypic assays where chemogenomic sets may be applied. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chemogenomics: Methods and Protocols is a valuable resource for all researchers who are interested in learning more about this diverse and developing field. 

 An Introduction to Chemogenomics.- Developing a Kinase Chemogenomic
Set: Facilitating Investigation into Kinase Biology by Linking Phenotypes to
Targets.- Compilation of Custom Compound/Bioactivity Datasets from Public
Repositories.- Quality Control of Chemogenomic Library using
LC-MS.- Annotation of the Effect of Chemogenomic Compounds on Cell Health
using High-Content Microscopy in Live-Cell Mode.- Characterization of
Cellular Viability using Label-Free Brightfield Live-Cell
Imaging.- Plate-Based Screening for DUB Inhibitors.- NanoBRET Live Cell
Kinase Selectivity Profiling Adapted for High Throughput Screening.- A
Fluorescence-Based Reporter Gene Assay to Characterize Nuclear Receptor
Modulators.- Measuring Protein-Protein Interactions in Cells using
NanoLuciferase Bioluminescence Resonance Energy Transfer (NanoBRET)
Assay.- HiBiT Cellular Thermal Shift Assay (HiBiT CETSA).- Detection of
Cellular Target Engagement for Small Molecule Modulators of Striatal-Enriched
Protein Tyrosine Phosphatase (STEP).- Target Deconvolution by Limited
Proteolysis Coupled to Mass Spectrometry.- Global Assessment of Drug Target
Engagement and Selectivity of Covalent Cysteine-Reactive Inhibitors using
Alkyne-Functionalized Probes.- 3D-Spheroid Invasion Assay for High-Throughput
Screening of Small Molecule Libraries.- Live-Cell High-Throughput Screen for
Monitoring Autophagy Flux.- Phenotypic Chemical Screening in CD4+ T Cells to
Identify Epigenetic Inhibitors.