Atjaunināt sīkdatņu piekrišanu

E-grāmata: Cilia: Structure and Motility

Volume editor , Volume editor (Professor, Department of Molecular Biology and Biophysics Director, Electron Microscopy Facility, University of Connecticut Health Center)
  • Formāts: EPUB+DRM
  • Sērija : Methods in Cell Biology
  • Izdošanas datums: 27-Nov-2009
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080961064
Citas grāmatas par šo tēmu:
  • Formāts - EPUB+DRM
  • Cena: 132,81 €*
  • * ši ir gala cena, t.i., netiek piemērotas nekādas papildus atlaides
  • Ielikt grozā
  • Pievienot vēlmju sarakstam
  • Šī e-grāmata paredzēta tikai personīgai lietošanai. E-grāmatas nav iespējams atgriezt un nauda par iegādātajām e-grāmatām netiek atmaksāta.
  • Formāts: EPUB+DRM
  • Sērija : Methods in Cell Biology
  • Izdošanas datums: 27-Nov-2009
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080961064
Citas grāmatas par šo tēmu:

DRM restrictions

  • Kopēšana (kopēt/ievietot):

    nav atļauts

  • Drukāšana:

    nav atļauts

  • Lietošana:

    Digitālo tiesību pārvaldība (Digital Rights Management (DRM))
    Izdevējs ir piegādājis šo grāmatu šifrētā veidā, kas nozīmē, ka jums ir jāinstalē bezmaksas programmatūra, lai to atbloķētu un lasītu. Lai lasītu šo e-grāmatu, jums ir jāizveido Adobe ID. Vairāk informācijas šeit. E-grāmatu var lasīt un lejupielādēt līdz 6 ierīcēm (vienam lietotājam ar vienu un to pašu Adobe ID).

    Nepieciešamā programmatūra
    Lai lasītu šo e-grāmatu mobilajā ierīcē (tālrunī vai planšetdatorā), jums būs jāinstalē šī bezmaksas lietotne: PocketBook Reader (iOS / Android)

    Lai lejupielādētu un lasītu šo e-grāmatu datorā vai Mac datorā, jums ir nepieciešamid Adobe Digital Editions (šī ir bezmaksas lietotne, kas īpaši izstrādāta e-grāmatām. Tā nav tas pats, kas Adobe Reader, kas, iespējams, jau ir jūsu datorā.)

    Jūs nevarat lasīt šo e-grāmatu, izmantojot Amazon Kindle.

Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella,  focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

* Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures



Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella.

* Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Papildus informācija

Updated classic and highly innovative techniques essential to the study of cilia and flagella
Contributors ix
Preface xi
Cryo-Electron Microscope Tomography to Study Axonemal Organization
Daniela Nicastro
Introduction and Rationale
2(10)
Methods and Materials
12(18)
Discussion
30(12)
References
32(10)
Electron Microscopic Imaging and Analysis of Isolated Dynein Particles
Anthony J. Roberts
Stan A. Burgess
Introduction
42(3)
Rationale
45(1)
Methods
46(13)
Summary
59(5)
References
60(4)
Immunogold Labeling of Flagellar Components In Situ
Stefan Geimer
Introduction
64(3)
Methods for Immunogold Labeling of Flagellar Components
67(7)
Materials
74(3)
Summary and Discussion
77(5)
References
78(4)
Scanning Electron Microscopy to Examine Cells and Organs
Jovenal T. San Agustin
John A. Follit
Gregory Hendricks
Gregory J. Pazour
Introduction
82(1)
Materials
82(2)
Metal-Impregnation Method for Scanning EM Analysis of Mouse Kidneys (modified from McManus et al., 1993)
84(2)
Method for Scanning EM Visualization of Mouse Nodal Cilia
86(1)
Summary
87(3)
References
87(3)
X-ray Fiber Diffraction Studies on Flagellar Axonemes
Kazuhiro Oiwa
Shinji Kamimura
Hiroyuki Iwamoto
Introduction
90(1)
Rationale
91(6)
Methods
97(8)
Results and Discussion
105(2)
Summary
107(5)
References
108(4)
Markers for Neuronal Cilia
Jacqueline S. Domire
Kirk Mykytyn
Introduction
112(1)
Immunoenzymatic Labeling of Neuronal Cilia in Brain Sections
113(2)
Immunofluorescent Labeling of Neuronal Cilia in Brain Sections
115(2)
Visualization of Neuronal Cilia In Vitro
117(3)
Summary
120(4)
References
121(3)
Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells
Heymut Omran
Niki T. Loges
Introduction
124(2)
Antibodies
126(3)
Materials and Methods
129(1)
Methods
129(2)
Discussion
131(4)
References
133(2)
Immunoprecipitation to Examine Protein Complexes
Gregory J. Pazour
Introduction
135(2)
General Procedure
137(2)
Controls for Immunoprecipitation Assays
139(2)
Summary
141(3)
References
142(2)
Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes
Karsten Boldt
Jeroen van Reeuwijk
Christian Johannes Gloeckner
Marius Ueffing
Ronald Roepman
Introduction
144(6)
Methods
150(5)
Data Evaluation
155(2)
Outlook
157(5)
References
158(4)
Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products
Miho Sakato
Chemical Crosslinking
162(2)
Identification of Interaction Partners
164(6)
Summary
170(4)
References
170(4)
Analysis of the Ciliary/Flagellar Beating of Chlamydomonas
Kenneth W. Foster
Introduction
174(8)
Rationale
182(1)
Techniques
182(49)
Future Directions
231(11)
References
232(10)
Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii
Ritsu Kamiya
Introduction
242(1)
Motility Assessments in Live Chlamydomonas Cells
242(4)
In Vitro Reactivation of Axonemal Beating
246(2)
Microtubule Sliding by Axonemal Dynein
248(8)
References
251(5)
High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain
Karl-Ferdinand Lechtreck
Michael J. Sanderson
George B. Witman
Introduction
256(1)
Materials and Equipment
257(2)
Methods
259(3)
Discussion
262(1)
Summary
262(4)
References
263(3)
Observation of Nodal Cilia Movement and Measurement of Nodal Flow
Yasushi Okada
Nobutaka Hirokawa
Introduction and Historical Background
266(3)
Observation of Nodal Cilia Motility in Mouse Embryo
269(7)
Observation of Nodal Flow in Mouse Embryo
276(2)
Observation of Nodal Cilia Motility and Nodal Flow in Rabbit Embryo
278(3)
Discussion
281(7)
References
284(4)
Modification of Mouse Nodal Flow by Applying Artificial Flow
Shigenori Nonaka
Introduction
288(1)
Solutions
289(1)
Experimental Setup
289(2)
Methods
291(4)
Discussion
295(5)
References
296(4)
Measuring Cilium-Induced Ca2+ Increases in Cultured Renal Epithelia
Helle A. Praetorius
Introduction
300(1)
Methods
300(10)
Materials
310(1)
Summary/Conclusion
311(4)
References
311(4)
Index 315(10)
Volume in Series 325
Stephen M. King is Professor of Molecular Biology and Biophysics at the University of Connecticut School of Medicine and is also director of the electron microscopy facility. He has studied the structure, function and regulation of dyneins for over 30 years using a broad array of methodologies including classical/molecular genetics, protein biochemistry, NMR structural biology and molecular modeling, combined with cell biological approaches, imaging and physiological measurements.