This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical
Laboratory.- System Performance Monitoring in Clinical Liquid
Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Tandem Mass Spectrometry
for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain
Organic Acidurias and Fatty Acid Oxidation Disorders.- Quantification of
Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid
Chromatography Electrospray Tandem Mass Spectrometry.- Quantitation of
Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass
Spectrometry (LC-MS/MS).- Comprehensive Determination of Amino Acids for
Diagnosis of Inborn Errors of Metabolism.- Quantification of Branched-Chain
Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass
Spectrometry (LC- MS/MS).- Quantitation of Butyrylcarnitine,
Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance
Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS).- Quantification
of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass
Spectrometry.- Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in
Negative Mode.- Measurement of Urine Free Cortisol and Cortisone by
LC-MS/MS.- Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by
Determining Creatine and Guanidinoacetate in Plasma and Urine.- Quantitation
of Estradiol and Testosterone in Serum Using LC-MS/MS.- Quantitation of Fatty
Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative
Chemical Ionization-Mass Spectrometry.- Quantitation of -Aminobutyric Acid
in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass
Spectrometry.- A Simple, Fast, and Reliable LC-MS/MS Method for the
Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine
Specimens.- Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and
Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry
(GC/MS).- Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra
Performance Liquid Chromatography Tandem Mass Spectrometry.- Quantitation of
IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
(LC-MS/MS).- Quantification of Insulin Analogs by Liquid Chromatography-High
Resolution Mass Spectrometry.- Differentiation of Common IGF-1 Variants Using
HRAM-MS COM Determination with Follow-Up MS/MS Verification.- Monitoring and
Identifying Insulin-Like Growth Factor 1 Variants by Liquid
Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory.-
Quantitation of Lactate in Cerebrospinal Fluid Using Liquid
Chromatography-Electrospray Tandem Mass Spectrometry.- Multiplex Lysosomal
Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry.-
Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem
Mass Spectrometry.- Detection of 13C-Mannitol and Other Saccharides Using
Tandem Mass Spectrometry for Evaluation ofIntestinal Permeability, or Leaky
Gut.- LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in
Serum, Plasma, and Urine: Method for Analyzing Isomers without
Chromatographic Separation.- Quantitation of 5-Methyltetrahydrofolate in
Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass
Spectrometry.- Screening of Organic Acidurias by Gas Chromatography Mass
Spectrometry (GC-MS).- Identification of Urine Organic Acids for the
Detection of Inborn Errors of Metabolism Using Urease and Gas
Chromatography-Mass Spectrometry (GC/MS).- Quantitative Organic Acids in
Urine by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry
(GCxGC-TOFMS).- Quantification of Parathyroid Hormone and Its Fragments in
Serum by Liquid Chromatography-High Resolution Mass Spectrometry.- High
Sensitivity Measurement of Parathyroid Hormone Related Protein (PTHrP) in
Plasma by LC-MS/MS.- Quantitation of Phenylalanine in Dried Blood Spot using
Liquid Chromatography TandemMass Spectrometry for Monitoring of Patients with
Phenylketonuria (PKU).- An Optimized Procedure for Proteomic Analysis of
Extracellular Vesicles Using In-Stage Tip Digestion and DIA LC-MS/MS:
Application to Liquid Biopsy in Cancer.- High-Throughput Plasma Proteomic
Profiling.- Quantitation of Purine in Urine by Ultra Performance Liquid
Chromatography-Tandem Mass Spectrometry.- Quantitation of Pyrimidine in Urine
by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry.-
Quantitation of Renin Activity in Plasma Using Liquid Chromatography-Tandem
Mass Spectrometry (LC-MS/MS).- Liquid Chromatography-Tandem Mass Spectrometry
(LC-MS/MS) Method for the Quantification Steroids Androstenedione,
Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and
Testosterone.- A User-Friendly Sample Preparation Alternative for Manual and
Automated LC-MS/MS Quantification of Testosterone.- Quantitation of
Thyroglobulin in Serum Using SISCAPA and Liquid Chromatography-Tandem Mass
Spectrometry (LC-MS/MS).- Quantitation of Free Thyroxine by Equilibrium
Dialysis and Liquid Chromatography-Tandem Mass Spectrometry.- Quantification
of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid
Chromatography-Tandem Mass Spectrometry.- Very Long-Chain Fatty Acids
Quantification by Gas-Chromatography Mass Spectrometry.- Quantification of
Very Long-Chain and Branched-Chain Fatty Acids in Plasma by Liquid
Chromatography-Tandem Mass Spectrometry.- High Throughput Analysis of
25-OH-Vitamin D2 and D3 Using Multi-Channel Liquid Chromatography-Tandem Mass
Spectrometry.- Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid
Chromatography-Tandem Mass Spectrometry.
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