This volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and no need for specialized reagents. The techniques described are a must for the measurement of many clinically relevant analytes in the fields of drug analysis, endocrinology, and inborn errors of metabolism. Each chapter provides a brief introduction about a specified analyte, followed by detailed instructions on the analytical protocol. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols is a great resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.
Recenzijas
This is a compendium of mass spectrometry protocols for clinically important analytes. This book is intended for anyone interested in clinical applications of mass spectrometry. It would be of interest to basic scientists, clinical laboratory practitioners (e.g., scientists, physicians, etc.), manufacturers, and healthcare providers interested in applications of mass spectrometry. This is your book if you are contemplating implementing mass spectrometry in your laboratory for any of these clinically important analytes. (Valerie L. Ng, Doody's Book Reviews, March, 2016)
Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular
Analysis.- Quantification of Free Carnitine and Acylcarnitines in Plasma or
Serum Using HPLC/MS/MS.- Quantification of Arginine and its Methylated
Derivatives in Plasma by High Performance Liquid Chromatography Tandem Mass
Spectrometry (LC-MS/MS).- Quantitation of Albumin in Urine by Liquid
Chromatography Tandem Mass Spectrometry.- Quantitation of Aldosterone in
Serum or Plasma Using Liquid Chromatography -Tandem Mass Spectrometry
(LC-MS/MS).- Quantification of Five Clinically Important Amino Acids by
HPLC-Triple TOF 5600 Based on Pre-Column Double Derivatization
Method.- Sensitive, Simple, and Robust Nano Liquid Chromatography-Mass
Spectrometry Method for Amyloid Protein Subtyping.- Quantitation of
Ubiquinone (Coenzyme Q10) in Serum/Plasma Using Liquid Chromatography
Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS).- Quantitative Analysis
of Salivary Cortisol Using LC-MS/MS.- Quantification of Dihydroxyacetone
Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600 Mass
Spectrometer.- Simultaneous Quantitation of Estradiol and Estrone in Serum
Using Liquid Chromatography Mass Spectrometry.- Direct Measurement of Free
Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid
Chromatography-Tandem Mass Spectrometry.- Quantification of -Aminobutyric
Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem
Mass Spectrometry.- Quantitation of Insulin Analogues in Serum Using
Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass
Spectrometry.- Quantitation of Insulin Like Growth Factor 1 in Serum by
Liquid Chromatography High Resolution Accurate-Mass Mass
Spectrometry.- Quantitation of Free Metanephrines in Plasma by Liquid
Chromatography Tandem Mass Spectrometry.- Quantification of Metanephrine and
Normetanephrine in Urine Using Liquid Chromatography Tandem Mass
Spectrometry.- High Throughput Analysis of Methylmalonic Acid in Serum,
Plasma and Urine by LC-MS/MS: Method for Analyzing Isomers without
Chromatographic Separation.- Quantitation of 5-Methyltetrahydrofolate in
Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass
Spectrometry.- Quantitative Organic Acids in Urine by Two Dimensional Gas
Chromatography Time of Flight Mass Spectrometry (GCxGC-TOFMS).- High
Sensitivity Measurement of Pancreatic Polypeptide and its Variant in Serum
and Plasma by LC-MS/MS.- Quantitation of Parathyroid Hormone in Serum or
Plasma by Liquid Chromatography Tandem Mass Spectrometry.- Determination of
Phenylalanine and Tyrosine by High Performance Liquid Chromatography Tandem
Mass Spectrometry.- Urine Purine Metabolite Determination by UPLC Tandem Mass
Spectrometry.- Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass
Spectrometry.- Quantitation of Plasma Renin Activity in Plasma Using Liquid
Chromatography -Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of
S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid
Chromatography Electrospray Tandem Mass Spectrometry.- A Simple, High
Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide
Trihexoside in Dried Blood Spots by LC/MS/MS.- Quantification of
Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone and
Testosterone by Liquid Chromatography Tandem Mass Spectrometry
(LC/MS/MS).- Urinary Succinylacetone Analysis by Gas Chromatography-Mass
Spectrometry (GC-MS).- Quantification of 1,25-Dihydroxyvitamin D2 & D3 in
Serum Using Liquid Chromatography Tandem Mass Spectrometry.- High-Throughput
Serum 25-Hydroxy Vitamin D Testing with Automated Sample
Preparation.- Quantitation of 25-OH-Vitamin-D2 and 25-OH-Vitamin-D3 in Urine
Using LC-MS/MS.
