Mass spectrometry is being used for a wide range of applications in the clinical laboratory. This text details step-by-step procedures of various mass spectrometry methods used for the analysis of an array of analytes including hormones, drugs and metabolites.
As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications. In Clinical Applications of Mass Spectrometry: Methods and Protocols, expert researchers provide detailed step-by-step procedures for the analysis of number of analytes of clinical importance. This versatile and expansive volume covers mass spectrometry methods for analytes including a variety of drugs, hormones, and metabolic compounds spanning the disciplines of toxicology, therapeutic drug monitoring, endocrinology, and pediatric metabolism. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to the analytes, lists of the necessary materials and reagents, readily reproducible analytical protocols, and detailed notes on troubleshooting and avoiding known pitfalls.
Comprehensive and dependable, Clinical Applications of Mass Spectrometry: Methods and Protocols offers its readers a wide array of valuable methods for experienced mass spectrometric labs that are looking to introduce new analyses as well as for those laboratories currently considering the addition of this resourceful and vital technology.
Mass spectrometry is being used for a wide range of applications in the clinical laboratory. This text details step-by-step procedures of various mass spectrometry methods used for the analysis of an array of analytes including hormones, drugs and metabolites.
Recenzijas
From the reviews:
This book provides a collection of over 50 applications of GC-MS, LC-MS, and ICP-MS for screening, identification, and quantification of clinically relevant analytes in body samples. It can be recommended to critical practitioners in laboratory medicine. (Hans H. Maurer, Analytical and Bioanalytical Chemistry, Vol. 399, 2011)
Clinical Applications of Mass Spectrometry Methods and Protocols is a compilation of ready-to-use protocols for the determination of clinically relevant analytes in various biological matrices. the book is a straightforward compilation of diverse protocols written in a very user-friendly format. It is especially valuable for labs that have just started implementing mass spectrometry or for those planning to do so in the near future. For labs with more experience in mass spectrometry, the protocols can save many hours of precious method development time. (Robert Jansen, Journal of the American Society for Mass Spectrometry, Vol. 22, 2011)
The Evolution of Mass Spectrometry in the Clinical Laboratory.-
Measurement of Plasma/Serum Acylcarnitines Using Tandem Mass Spectrometry.-
Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of
Metabolism.- Identification and Quantitation of Amphetamine, Methamphetamine,
MDMA, Pseudoephedrine, and Ephedrine in Blood, Plasma, and Serum Using Gas
Chromatography-Mass Spectrometry (GC/MS).- Quantification of Antidepressants
Using Gas Chromatography-Mass Spectrometry.- Quantitation of Argatroban in
Plasma using Liquid Chromatography Electrospray Tandem Mass Spectrometry
(UPLC-ESI-MS/MS).- Quantitation of Amobarbital, Butalbital, Pentobarbital,
Phenobarbital, and Secobarbital in Urine, Serum, and Plasma Using Gas
Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Benzodiazepines in
Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- LC-MS/MS
Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and
Meconium.- Simultaneous Determination and Quantification of 12
Benzodiazepines in Serum or Whole Blood Using UPLC/MS/MS.- Determination of
Benzoic Acid in Serum or Plasma by Gas Chromatography-Mass Spectrometry
(GC/MS).- Quantification of Busulfan in Plasma Uing Liquid Chromatography
Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).- Quantitation of
Total 11-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol in Urine and Blood Using
Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Cocaine,
Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood
Using Gas Chromatography-Mass Spectrometry (GC-MS).- Identification and
Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum,
and Plasma Using Ultra-Performance Liquid Chromatography Coupled to Tandem
Mass Spectrometry (UPLC-MS/MS).- Detection and Quantification of Cocaine and
Benzoylecgonine in Meconium Using Solid Phase Extraction and UPLC/MS/MS.-
Diagnosis of Creatine Metabolism Disorders by Determining Creatine and
Guanidinoacetate in Plasma and Urine.- Broad Spectrum Drug Screening Using
Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCMS).- Broad
Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole
Linear Ion Trap Mass Spectrometry.- High Sensitivity Measurement of Estrone
and Estradiol in Serum and Plasma Using LC-MS/MS.- 3-Hydroxy-Fatty Acid
Analysis by Gas Chromatography-Mass Spectrometry.- Quantitation of Fentanyl
in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).-
Determination of Total Homocysteine in Plasma Using Liquid Chromatography
Coupled to Tandem Mass Spectrometry (LC/MS/MS).- Quantitation of Homovanillic
Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine Using Gas
Chromatography-Mass Spectrometry (GC/MS).- Quantitation of 17-OH-Progesterone
(OHPG) for Diagnosis of Congenital Adrenal Hyperplasia (CAH).- Determination
of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry
(GC/MS).- Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass
Spectrometry (GC-MS).- Quantitation of Indomethacin in Serum and Plasma Using
Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Iothalamate in
Urine and Plasma Using Liquid Chromatography Electrospray Tandem Mass
Spectrometry (HPLC-ESI-MS/MS).- Identification and Quantitation of Ketamine
in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS).-
Measurement of Filter Paper Bloodspot Lead by Inductively Coupled Plasma-Mass
Spectrometry (ICP-MS).- Multiplex Lysosomal Enzyme Activity Assay on Dried
Blood Spots Using Tandem Mass Spectrometry.- Gas Chromatography-Mass
Spectrometry Method for the Determination of Methadone and
2-Ethylidene-1,5-Dimethyl-3,3-Diphenylpyrrolidine (EDDP).- Liquid
Chromatography-Tandem Mass Spectrometry (LC-MSMS) Method for Monitoring
Methotrexate in the Setting of Carboxypeptidase-G2 Therapy.- Quantitation of
Methylmalonic Acid in Serum or Plasma Using Isotope Dilution-Selected Ion Gas
Chromatography-Mass Spectrometry.- Quantitation of Methylmalonic Acid in
Plasma Using Liquid ChromatographyTandem Mass Spectrometry.- Monitoring of
Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry.-
Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in
Urine, Serum, and Plasma Using LC-MS/MS.- Quantitation of Opioids in Blood
and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS).- Quantitation
of Morphine, Codeine, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone, and
6-Monoacetylmorphine (6-MAM) in Urine, Blood, Serum, or Plasma Using Liquid
Chromatography with Tandem Mass Spectrometry Detection.- Urine Organic Acid
Analysis for Inherited Metabolic Disease Using Gas Chromatography-Mass
Spectrometry.- Identification of Urine Organic Acids for the Detection of
Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass
Spectrometry (GC/MS).- Quantitation of Orotic Acid in Urine Using Isotope
Dilution-Selected Ion Gas Chromatography-Mass Spectrometry.- Quantitation of
Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry
(GC-MS).- Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas
Chromatography-Mass Spectrometry (GC-MS).- Quantitation of Sirolimus Using
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS).- Quantitation of
Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem
Mass Spectrometry (HPLC/MS/MS).- Analysis of Testosterone in Serum Using Mass
Spectrometry.- Determination of Tetrahydrozoline in Urine and Blood Using Gas
Chromatography-Mass Spectrometry (GC/MS).- Quantitation of 25-OH-Vitamin D
(25OHD) Using Liquid Tandem Mass Spectrometry (LC-MS/MS).- Identification and
Quantitation of Zolpidem in Biological Matrices Using Gas Chromatography-Mass
Spectrometry (GC-MS).- Identification and Quantitation of Zopiclone in
Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS).
