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E-grāmata: Two-Component Signaling Systems, Part A

Volume editor (The Salk Institute, La Jolla, CA, USA), Volume editor (Cornell University, Ithaca, NY, USA), Volume editor (Cornell University, Ithaca, NY, USA)
  • Formāts: EPUB+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 21-Sep-2011
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080548715
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Two-Component Signaling Systems, Part APalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Enzymology
  • Izdošanas datums: 21-Sep-2011
  • Izdevniecība: Academic Press Inc
  • Valoda: eng
  • ISBN-13: 9780080548715
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Biochemists and researchers in related life science fields.

Key Features:
* Presents detailed protocols
* Includes troubleshooting tips

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections include:
Computational Analyses of Sequences and Sequence Alignments
Biochemical and Genetic Assays of Individual Components of Signaling Systems
Physiological Assays and Readouts

* Presents detailed protocols
* Includes troubleshooting tips

Papildus informācija

Fueled by the explosion of sequence information provided by various genome projects, the number of two-component systems continues to rapidly grow.
CONTRIBUTORS TO VOLUME 422 ix
VOLUMES IN SERIES xiii
Section I. Computational Analyses of Sequences and Sequence Alignments
1. Comparative Genomic and Protein Sequence Analyses of a Complex System Controlling Bacterial Chemotaxis
KRISTIN WUICHET, ROGER P. ALEXANDER, AND IGOR B. ZHULIN
3
2. Two-Component Systems in Microbial Communities: Approaches and Resources for Generating and Analyzing Metagenomic Data Sets
MIRCEA PODAR
32
3. Identification of Sensory and Signal-Transducing Domains in Two-Component Signaling Systems
MICHAEL Y. GALPERIN AND ANASTASIA N. NIKOLSKAYA
47
4. Features of Protein–Protein Interactions in "Iwo-Component Signaling Deduced from Genomic Libraries
ROBERT A. WHITE, HENDRIK SZURMANT, JAMES A. HOCH, AND TERENCE HWA
75
5. Sporulation Phosphorelay Proteins and Their Complexes: Crystallographic Characterization
KOTTAYIL I. VARUGHESE, HAIYAN ZHAO, VIDYA HARINI VELDORE, AND JAMES ZAPF
102
6. Control Analysis of Bacterial Chemotaxis Signaling
TAU-MU YI, BURTON W. ANDREWS, AND PABLO A. IGLESIAS
123
7. Classification of Response Regulators Based on "Their Surface Properties
DOUGLAS J. KOJETIN, DANIEL M. SULLIVAN, RICHELE J. THOMPSON, AND JOHN CAVANAGH
141
Section II. Biochemical and Genetic Assays of Individual Components of Signaling Systems
8. Purification and Assays of Rhodobacter capsulatus RegB–RegA Two-Component Signal Transduction System
LEE R. SWEM, DANIELLE L. SWEM, JIANG WU, AND CARL E. BAUER
173
9. Purification and Reconstitution of PYP-Phytochrome with Biliverdin and 4-Hydroxycinnamic Acid
YOUNG-HO CHUNG, SHINJI MASUDA, AND CARL E. BAUER
184
10. Oxygen and Redox Sensing by Two-Component Systems That Regulate Behavioral Responses: Behavioral Assays and Structural Studies of Aer Using In Vivo Disulfide Cross-Linking
BARRY L. TAYLOR, KYLIE J. WATTS, AND MARK S. JOHNSON
190
11. Two-Component Signaling in the Virulence of Staphylococcus aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence
KENJI KUROKAWA, CHIKARA KAITO, AND KAZUHISA SEKIMIZU
233
12. TonB System, In Vivo Assays and Characterization
KATHLEEN POSTLE
245
13. Biochemical Characterization of Plant Ethylene Receptors Following Transgenic Expression in Yeast
G. ERIC SCHALLER AND BRAD M. BINDER
270
14. Structure of SixA, a Histidine Protein Phosphatase of the ArcB HistidineContaining Phosphotransfer Domain in Escherichia coli
TOSHIO HAKOSHIMA AND HISAKO ICHIHARA
288
15. Triggering and Monitoring Light-Sensing Reactions in Protein Crystals
PIERRE-DAMIEN COUREUX AND ULRICH K. GENICK
305
16. Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
CHRISTOPHER J. HALKIDES, CORY J. BOTTONE, ERIC S. CASPER, R. MATTHEW HAAS, AND KENNETH MCADAMS
338
17. Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions
S. THOMAS KING AND LINDA J. KENNEY
352
18. Gene Promoter Scan Methodology for Identifying and Classifying Coregulated Promoters
IGOR ZWIR, OSCAR HARARI, AND EDUARDO A. GROISMAN
361
19. Targeting Two-Component Signal Transduction: A Novel Drug Discovery System
ARID OKADA, YASUHIRO GOTOH, TAKAFUMI WATANABE, EIJI FURUTA, KANEYOSHI YAMAMOTO, AND RYUTARO UTSUMI
386
20. The Essential YycFG Two-Component System of Bacillus subtilis
HENDRIK SZURMANT, TATSUYA FUKUSHIMA, AND JAMES A. HOCH
396
Section III. Physiological Assays and Readouts
21. Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry, and Cell Tracking
MD. A. MOTALEB, MICHAEL R. MILLER, RICHARD G. BAKKER, CHUNHAO LI, AND NYLES W. CHARON
421
22. Phosphorylation Assays of Chemotaxis Two-Component System Proteins in Borrelia burgdorferi
MD. A. MOTALEB, MICHAEL R. MILLER, CHUNHAO LI, AND NYLES W. CHARON
438
23. Regulation of Respiratory Genes by ResD–ResE Signal Transduction System in Bacillus subtilis
HAO GENG, PETER ZUBER, AND MICHIKO M. NAKANO
448
24. Detection and Measurement of Two-Component Systems That Control Dimorphism and Virulence in Fungi
JULIE C. NEMECEK, MARCEL WUTHRICH, AND BRUCE S. KLEIN
465
25. Using Two-Component Systems and Other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices
ALEXANDER J. NINFA, STEPHEN SELINSKY, NICOLAS PERRY, STEPHEN ATKINS, QI XIU SONG, AVI MAYO, DAVID ARPS, PETER. WOOLF, AND MARIETTE R. ATKINSON
488
AUTHOR INDEX 513
SUJECT INDEX 541


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