This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to µm3) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.
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1 | (10) |
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Overview of SMFS Techniques |
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2 | (1) |
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Experiments Using Single-Molecule AFM |
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3 | (2) |
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Limitations of AFM-Based SMFS |
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5 | (4) |
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9 | (2) |
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2 Correlation Force Spectroscopy |
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11 | (14) |
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Correlation Force Spectroscopy: Rationale |
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11 | (1) |
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12 | (5) |
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Laterally Offset Configuration |
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14 | (1) |
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Vertically Offset Configuration |
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15 | (2) |
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Analysis of Correlations Between Two Cantilevers |
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17 | (2) |
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Validation of Fluctuation-Dissipation Theorem for One Cantilever |
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19 | (1) |
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Analysis of Thermal Fluctuations to Obtain Correlations |
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19 | (3) |
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22 | (3) |
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3 Dynamics of Single Molecules |
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25 | (14) |
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Elastic Properties of Single Molecules: Worm-Like Chain and Freely Jointed Chain Models |
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25 | (3) |
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Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model |
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28 | (4) |
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32 | (3) |
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Rouse with Internal Friction |
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35 | (1) |
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Model of Linear Viscoelasticity of a Semiflexible Chain |
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36 | (2) |
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38 | (1) |
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4 Microrheology with Correlation Force Spectroscopy |
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39 | (8) |
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Existing Techniques of Rheometry |
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39 | (1) |
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40 | (1) |
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Comparison to FE Analysis |
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40 | (2) |
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Comparison to Simple Harmonic Oscillator Model |
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42 | (2) |
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44 | (3) |
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5 Development of Colloidal Probe Correlation Force Spectroscopy: Case Study |
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47 | (16) |
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51 | (4) |
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53 | (2) |
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55 | (5) |
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60 | (2) |
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62 | (1) |
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6 Correlation Force Spectroscopy for Single-Molecule Measurements |
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63 | (8) |
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Effect of the Distance Between Cantilever Tips |
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63 | (2) |
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Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy |
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65 | (4) |
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69 | (2) |
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7 Single-Molecule Force Spectroscopy of Dextran |
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71 | (12) |
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71 | (1) |
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72 | (8) |
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Stretching a Dextran Molecule |
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72 | (2) |
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Static Force-Elongation Mode |
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74 | (3) |
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77 | (3) |
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80 | (1) |
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81 | (2) |
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8 Single Molecule Force Spectroscopy of Single-Stranded DNA |
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83 | (18) |
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83 | (1) |
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83 | (3) |
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86 | (2) |
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88 | (5) |
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93 | (3) |
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96 | (3) |
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99 | (2) |
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101 | (4) |
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101 | (1) |
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102 | (3) |
| Appendices |
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105 | (6) |
| References |
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Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva. His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces.
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