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CRISPR Guide RNA Design: Methods and Protocols 2021 ed. [Mīkstie vāki]

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  • Formāts: Paperback / softback, 286 pages, height x width: 254x178 mm, weight: 566 g, 46 Illustrations, color; 10 Illustrations, black and white; X, 286 p. 56 illus., 46 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2162
  • Izdošanas datums: 16-Sep-2021
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071606891
  • ISBN-13: 9781071606896
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  • Mīkstie vāki
  • Cena: 118,31 €*
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CRISPR Guide RNA Design: Methods and Protocols 2021 ed.Palielināt
  • Formāts: Paperback / softback, 286 pages, height x width: 254x178 mm, weight: 566 g, 46 Illustrations, color; 10 Illustrations, black and white; X, 286 p. 56 illus., 46 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2162
  • Izdošanas datums: 16-Sep-2021
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071606891
  • ISBN-13: 9781071606896
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071606896.html
Keywords:
This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.
Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR
Experiments.- Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and
Cas-Analyzer.- Chemical Modification of Guide RNAs for Improved CRISPR
Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells.- Gene
Disruption Using Chemically-Modified CRISPR-Cpf1 RNA.- Split-and-Click
sgRNA.- Chimeric DNA-RNA Guide RNA Designs.- Harnessing tRNA for Processing
Ability and Promoter Activity.- Targeting Noncoding RNA Domains to Genomic
Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing
sgRNAncRNA Expression Constructs.- Controlling the Activity of CRISPR
Transcriptional Regulators with Inducible sgRNAs.- Gene Manipulation Using
Fusion Guide RNAs for Cas9 and Cas12a.- Methods for Measuring CRISPR/Cas9 DNA
Cleavage in Cells.- In Vitro Assays for Comparing the Specificity of First-
and Next-Generation CRISPR/Cas9 Systems.- Profiling Genome-Wide Specificity
of CRISPR-Cas9 Using Digenome-Seq.- Detection of CRISPR/Cas9-Generated
Off-Target Effect by Integration-Defective Lentiviral Vector.- Genome-Wide
CRISPR Off-Target DNA Break Detection by the BLISS Method.