E-grāmata: Cryopreservation and Freeze-Drying Protocols

This compilation of new research on one of the most important and frustrating aspects of research in this field has been completely updated and features notes for each protocol, methods for conservation and sustainable use of resources, methods for lyophilization of proteins, and new and new techniques for preserving biological materials. Its 23 articles range from the freeze-drying of yeast cultures and other materials to the cryopreservation of bird or fish sperm right up to the preservation of mammalian oocytes, semen and embryos. Each of the articles includes well-chosen lists of resources and further reading, and the collection includes a glossary of specialized terms. Annotation ©2007 Book News, Inc., Portland, OR (booknews.com)

Cryopreservation and Freeze-Drying Protocols: Second Edition is a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. This widely expanded second edition includes novel approaches and protocols for biological materials that were not preservable when the first edition was published in 1995.This volume begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Detailed descriptions of materials required and stepwise protocols are presented for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.

This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Coverage presents detailed descriptions of the materials required and stepwise protocols for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.