Preface |
|
v | |
Contributors |
|
xi | |
|
Part I Modern Quantitative Virological Methods |
|
|
|
1 Dengue Virus Growth, Purification, and Fluorescent Labeling |
|
|
3 | (12) |
|
|
|
|
|
2 Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique |
|
|
15 | (12) |
|
|
|
3 Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique |
|
|
27 | (14) |
|
|
|
4 Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy |
|
|
41 | (12) |
|
|
|
5 Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion |
|
|
53 | (22) |
|
|
|
6 Pseudo-infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection |
|
|
75 | (24) |
|
|
|
|
7 Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High-Content Imaging |
|
|
99 | (14) |
|
|
|
|
|
|
Part II Reverse Genetic Systems to Study Virus Replication and Evolution |
|
|
|
8 Development and Application of Dengue Virus Reverse Genetic Systems |
|
|
113 | (18) |
|
|
9 Construction of Self-Replicating Subgenomic Dengue Virus 4 (DENV4) Replicon |
|
|
131 | (20) |
|
|
|
Radhakrishnan Padmanabhan |
|
|
10 Targeted Mutagenesis of Dengue Virus Type 2 Replicon RNA by Yeast In Vivo Recombination |
|
|
151 | (10) |
|
|
Radhakrishnan Padmanabhan |
|
|
11 Identification of Dengue-Specific Human Antibody Fragments Using Phage Display |
|
|
161 | (14) |
|
|
|
|
|
12 Next-Generation Whole Genome Sequencing of Dengue Virus |
|
|
175 | (24) |
|
|
|
|
|
|
|
|
Part III Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions |
|
|
|
13 MPGAfold in Dengue Secondary Structure Prediction |
|
|
199 | (26) |
|
|
|
14 Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE |
|
|
225 | (16) |
|
|
|
15 Use of Small-Angle X-ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5 |
|
|
241 | (12) |
|
|
|
16 Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry |
|
|
253 | (18) |
|
|
|
|
17 Analysis of Affinity of Dengue Virus Protein Interaction Using Biacore |
|
|
271 | (14) |
|
|
|
18 Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation |
|
|
285 | (16) |
|
|
|
|
|
|
|
|
19 Investigating Dengue Virus Nonstructural Protein 5 (NS5) Nuclear Import |
|
|
301 | (30) |
|
|
|
|
|
|
Part IV In Vitro Enzyme Assays for Antiviral Screening |
|
|
|
20 Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High-Throughput Screening |
|
|
331 | (14) |
|
|
|
Radhakrishnan Padmanabhan |
|
|
21 Construction of Dengue Virus Protease Expression Plasmid and In Vitro Protease Assay for Screening Antiviral Inhibitors |
|
|
345 | (16) |
|
|
|
Radhakrishnan Padmanabhan |
|
|
22 Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase |
|
|
361 | (16) |
|
Siwaporn Boonyasuppayakorn |
|
|
Radhakrishnan Padmanabhan |
|
|
Part V Dengue Animal Model for Vaccines and Antivirals |
|
|
|
23 Animal Models in Dengue |
|
|
377 | (14) |
|
|
|
24 Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model |
|
|
391 | |
|
|
Erratum to |
|
1 | (400) |
Index |
|
401 | |