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E-grāmata: Dengue: Methods and Protocols

  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1138
  • Izdošanas datums: 02-Apr-2014
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493903481
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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 1138
  • Izdošanas datums: 02-Apr-2014
  • Izdevniecība: Humana Press Inc.
  • Valoda: eng
  • ISBN-13: 9781493903481

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Infection by flaviviruses such as dengue virus serotypes (DENV 1-4), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE), yellow fever virus (YFV) and West Nile virus (WNV) impact millions of lives and cause tens of thousands of mortalities each year. Dengue is a global public health emergency especially since there is no preventative vaccine or antiviral treatment for dengue disease.Dengue: Methods and Protocols offers the increasing number of dengue researchers a one-stop protocol book with techniques compiled from the leading laboratories working on dengue. Chapters cover topics such as dengue virus isolation from clinical samples, quantification of human antibodies against the virus, assays to quantify the virus particles, the widely used mouse model to study dengue pathogenesis, vaccine and antiviral efficacies. Written in the successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Dengue: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies on dengue research.

Preface v
Contributors xi
Part I Modern Quantitative Virological Methods
1 Dengue Virus Growth, Purification, and Fluorescent Labeling
3(12)
Summer Zhang
Kuan Rong Chan
Hwee Cheng Tan
Eng Eong Ooi
2 Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique
15(12)
Milly M. Choy
Duane J. Gubler
3 Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique
27(14)
Ruklanthi de Alwis
Aravinda M. de Silva
4 Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy
41(12)
Joanne L. Tan
Shee Mei Lok
5 Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion
53(22)
Kaiting Ng
John E. Connolly
6 Pseudo-infectious Reporter Virus Particles for Measuring Antibody-Mediated Neutralization and Enhancement of Dengue Virus Infection
75(24)
Swati Mukherjee
Theodore C. Pierson
Kimberly A. Dowd
7 Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High-Content Imaging
99(14)
Kah Hin Tan
Kitti Chan Wing Ki
Satoru Watanabe
Subhash G. Vasudevan
Manoj Krishnan
Part II Reverse Genetic Systems to Study Virus Replication and Evolution
8 Development and Application of Dengue Virus Reverse Genetic Systems
113(18)
Andrew D. Davidson
9 Construction of Self-Replicating Subgenomic Dengue Virus 4 (DENV4) Replicon
131(20)
Sofia L. Alcaraz-Estrada
Rosa del Angel
Radhakrishnan Padmanabhan
10 Targeted Mutagenesis of Dengue Virus Type 2 Replicon RNA by Yeast In Vivo Recombination
151(10)
Mark Manzano
Radhakrishnan Padmanabhan
11 Identification of Dengue-Specific Human Antibody Fragments Using Phage Display
161(14)
Moon Y.F. Tay
Chin Chin Lee
Subhash G. Vasudevan
Nicole J. Moreland
12 Next-Generation Whole Genome Sequencing of Dengue Virus
175(24)
Pauline Poh Kim Aw
Paola Florez de Sessions
Andreas Wilm
Long Truong Hoang
Niranjan Nagarajan
October M. Sessions
Martin Lloyd Hibberd
Part III Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions
13 MPGAfold in Dengue Secondary Structure Prediction
199(26)
Wojciech K. Kasprzak
Bruce A. Shapiro
14 Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE
225(16)
Joanna Sztuba-Solinska
Stuart F.J. Le Grice
15 Use of Small-Angle X-ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5
241(12)
Kyung H. Choi
Marc Morais
16 Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry
253(18)
Alex M. Ward
J. Gunaratne
Mariano A. Garcia-Blanco
17 Analysis of Affinity of Dengue Virus Protein Interaction Using Biacore
271(14)
Yin Hoe Yau
Susana Geifman Shochat
18 Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation
285(16)
Nicholas J. Barrows
Sharon F. Jamison
Shelton S. Bradrick
Caroline Le Sommer
So Young Kim
James Pearson
Mariano A. Garcia-Blanco
19 Investigating Dengue Virus Nonstructural Protein 5 (NS5) Nuclear Import
301(30)
Johanna E. Fraser
Stephen M. Rawlinson
Chunxiao Wang
David A. Jans
Kylie M. Wagstaff
Part IV In Vitro Enzyme Assays for Antiviral Screening
20 Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High-Throughput Screening
331(14)
Mark Manzano
Janak Padia
Radhakrishnan Padmanabhan
21 Construction of Dengue Virus Protease Expression Plasmid and In Vitro Protease Assay for Screening Antiviral Inhibitors
345(16)
Huiguo Lai
Tadahisa Teramoto
Radhakrishnan Padmanabhan
22 Construction of Plasmid, Bacterial Expression, Purification, and Assay of Dengue Virus Type 2 NS5 Methyltransferase
361(16)
Siwaporn Boonyasuppayakorn
Radhakrishnan Padmanabhan
Part V Dengue Animal Model for Vaccines and Antivirals
23 Animal Models in Dengue
377(14)
Emily Plummer
Sujan Shresta
24 Evaluation of Dengue Antiviral Candidates In Vivo in Mouse Model
391
Satoru Watanabe
Subhash G. Vasudevan
Erratum to 1(400)
Index 401