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Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols Second Edition 2021 [Hardback]

  • Formāts: Hardback, 309 pages, height x width: 254x178 mm, weight: 816 g, 48 Illustrations, color; 27 Illustrations, black and white; XIII, 309 p. 75 illus., 48 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2219
  • Izdošanas datums: 20-Oct-2020
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071609734
  • ISBN-13: 9781071609736
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  • Formāts: Hardback, 309 pages, height x width: 254x178 mm, weight: 816 g, 48 Illustrations, color; 27 Illustrations, black and white; XIII, 309 p. 75 illus., 48 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2219
  • Izdošanas datums: 20-Oct-2020
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071609734
  • ISBN-13: 9781071609736
Citas grāmatas par šo tēmu:
This detailed second edition presents a wide variety of marine invertebrate model systems, from cephalochordata to holothurians, along with novel experimental protocols for taking advantage of their unique properties. The techniques range from culturing the organisms to modifying their DNA. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, Second Edition is an ideal guide for researchers working with these versatile organisms and for furthering our understanding of fundamental biological questions.
Preface v
Contributors xi
1 Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)
1(30)
Linda Z. Holland
Guang Li
2 Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata
31(18)
Ryusaku Deguchi
Takeshi Nakano
3 The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
49(20)
Naoyuki Murabe
Ei-ichi Okumura
Kazuyoshi Chiba
Enako Hosoda
Susumu Ikegami
Takeo Kishimoto
4 Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis
69(12)
Aldine R. Amiel
Eric Rbttinger
5 Staining and Tracking Methods for Studying Sponge Cell Dynamics
81(18)
Carole Borchiellini
Sandie M. Degnan
Emilie Le Goff Caroline Rocher
Amelie Vernale
Stephen Baghdiguian
Nina Sejourne
Florent Marschal
Andre Le Bivic
Nelly Godefroy
Bernard M. Degnan
Emmanuelle Renard
6 Microscopy Studies of Placozoans
99(20)
Carolyn L. Smith
Tatiana D. Mayorova
Christine A. Winters
Thomas S. Reese
Sally P. Leys
Andreas Heyland
7 Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata
119(18)
Lauren Bates
Emily Wiseman
Jamie Kitson
David J. Carroll
8 Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging
137(14)
Stephen A. Strieker
9 Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization
151(12)
Emily Wiseman
Lauren Bates
David J. Carroll
10 Quantifying Cell Proliferation During Regeneration of Aquatic Worms
163(18)
Eduardo E. Zattara
B. Duygu Ozpolat
11 In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis
181(14)
Laura Fierro-Constain
Caroline Rocher
Florent Marschal
Quentin Schenkelaars
Nina Sejourne
Carole Borchiellini
Ewmanulle Renard
12 Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond
195(22)
Margherita Perillo
Periklis Paganos
Maxwell Spurrell
Maria I. Arnone
Gary M. Wessel
13 Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation
217(14)
Alex McDougall
Celine Hebras
Isa Gomes
Remi Dumollard
14 Transcriptomic Analysis in the Sea Anemone Nematostella vectensis
231(10)
Jacob F. Warner
Eric Rbttinger
15 RNA Interference on Regenerating Holothurian Gut Tissues
241(12)
Miosotis Alicea-Delgado
Samir A. Bello-Melo
Jose E. Garcta-Arraras
16 ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos
253(14)
Marta S. Magri
Danila Voronov
Jovana Ranaelovic
Claudia Cuomo
Jose Luis Gomez-Skarmeta
Maria I. Arnone
17 Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase
267(10)
Sonoko Kinjo
Masato Kiyomoto
Takashi Yamamoto Kazubo Ikeo
Sbunsuke Yaguchi
18 Functional Studies of Trichoplax adhaerens Voltage-Gated Calcium Channel Activity
277(12)
Julia Gauberg
Adriano Senatore
Andreas Heyland
19 A Bioinformatics Tutorial for Comparative Genomics of Meiofauna
289
Joseph L. Sevigny
Jon L. Norenburg
Francesca Leasi
Correction to: A Bioinformatics Tutorial for Comparative Genomics of Meiofauna 1(306)
Index 307