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DNA and RNA Isolation Techniques for Non-Experts 2022 ed. [Hardback]

  • Formāts: Hardback, 203 pages, height x width: 235x155 mm, weight: 500 g, 48 Illustrations, color; 10 Illustrations, black and white; XVI, 203 p. 58 illus., 48 illus. in color., 1 Hardback
  • Sērija : Techniques in Life Science and Biomedicine for the Non-Expert
  • Izdošanas datums: 30-Mar-2022
  • Izdevniecība: Springer Nature Switzerland AG
  • ISBN-10: 3030942295
  • ISBN-13: 9783030942298
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DNA and RNA Isolation Techniques for Non-Experts 2022 ed.Palielināt
  • Formāts: Hardback, 203 pages, height x width: 235x155 mm, weight: 500 g, 48 Illustrations, color; 10 Illustrations, black and white; XVI, 203 p. 58 illus., 48 illus. in color., 1 Hardback
  • Sērija : Techniques in Life Science and Biomedicine for the Non-Expert
  • Izdošanas datums: 30-Mar-2022
  • Izdevniecība: Springer Nature Switzerland AG
  • ISBN-10: 3030942295
  • ISBN-13: 9783030942298
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9783030942298.html
Keywords:
This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
Part I Introductory
Chapters
Basic Idea About Nucleic Acids for Nonexperts
3(20)
1 Molecules of Life
3(1)
2 Nucleic Acids
4(4)
3 DNA
8(6)
3.1 Secondary Structure of DNA: The Double Helix
9(1)
3.2 Other Secondary Structures of DNA/Types of DNA
10(1)
3.3 Physical and Chemical Properties of DNA
11(1)
3.4 From DNA to Chromosome
12(2)
3.5 Functions of DNA
14(1)
4 RNA
14(9)
4.1 Structure
15(1)
4.2 Types of RNA
16(4)
4.3 Functions of RNA
20(1)
References
21(2)
General Steps During Isolation of DNA and RNA
23(10)
1 Introduction
23(1)
2 Common Steps of Nucleic Acid Isolation
24(2)
3 Widely Used Extraction Methods
26(1)
4 DNA Extraction and Purification Based on Source Materials
27(1)
4.1 DNA Isolation from Microbes
27(1)
4.2 DNA Extraction from Animal Cells and Tissues
28(1)
4.3 DNA Extraction from Plant Tissues and Cells
28(1)
5 RNA Isolation
28(5)
References
29(4)
Part II Methods
Phenol-Chloroform DNA Isolation Method
33(8)
1 Introduction of the Technique
33(1)
2 Basic Principle
34(1)
3 Protocol
34(3)
3.1 Reagents Required
34(1)
3.2 Working Solutions
35(1)
3.3 Procedure
35(2)
4 Precautions
37(1)
5 Applications
38(3)
5.1 Gene Sequencing
38(1)
5.2 Genetic Engineering
38(1)
References
39(2)
RNA Isolation by the Guanidinium-Acid-Phenol Method
41(6)
1 Introduction
41(1)
2 Basic Principle
42(1)
3 Protocol
42(3)
3.1 Reagents Required
42(1)
3.2 Working Solutions
43(1)
3.3 Procedure
43(2)
4 Precautions
45(1)
5 Applications
46(1)
References
46(1)
Spin Column-Based Isolation of Nucleic Acid
47(8)
1 Introduction
47(1)
2 Basic Principle
48(1)
3 Protocol
49(2)
3.1 Reagents Required
49(1)
3.2 Working Solution
50(1)
3.3 Procedure
50(1)
4 Precautions
51(1)
5 Applications
52(3)
References
52(3)
Isolation of Plasmid DNA by Alkaline Lysis
55(8)
1 Introduction
55(1)
2 Basic Principle
56(1)
3 Protocol
57(3)
3.1 Reagents Required
57(1)
3.2 Working Solution
58(1)
3.3 Procedure
58(2)
4 Precautions
60(1)
5 Applications
60(3)
References
61(2)
DNA Isolation by Hydrophilic Ionic Liquid Treatment
63(6)
1 Introduction
63(1)
2 Basic Principle
64(1)
3 Protocol
65(1)
3.1 Reagents Required
65(1)
3.2 Working Solutions
65(1)
3.3 Procedure
65(1)
4 Precautions
66(1)
5 Applications
67(2)
5.1 Food Safety Surveillance
67(1)
5.2 PCR Enhancement
67(1)
References
68(1)
Lithium Chloride-Based Isolation of RNA
69(4)
1 Introduction
69(1)
2 Basic Principle
70(1)
3 Protocol
70(1)
3.1 Reagents Required
70(1)
3.2 Working Solution
70(1)
3.3 Procedure
71(1)
4 Precautions
71(1)
5 Applications
71(2)
References
72(1)
Isolation of Bacteriophage DNA by PEG Method
73(6)
1 Introduction
73(1)
2 Basic Principle
74(1)
3 Protocol
74(2)
3.1 Reagents Required
74(1)
3.2 Working Solutions
75(1)
3.3 Procedure
75(1)
4 Precautions
76(1)
5 Applications
77(2)
5.1 Genetic Engineering
77(1)
5.2 Molecular Studies
77(1)
5.3 Vaccine Development
77(1)
References
78(1)
DNA Isolation by Chelex Method
79(6)
1 Introduction
79(1)
1.1 Basic Principle
80(1)
2 Protocol
80(2)
2.1 Reagents Required
80(1)
2.2 Working Solutions
81(1)
2.3 Procedure
81(1)
3 Precautions
82(1)
4 Applications
83(2)
4.1 Forensic Science
83(1)
4.2 Pathology
83(1)
4.3 Gene Sequencing
83(1)
References
84(1)
DNA Isolation by Lysozyme and Proteinase K
85(4)
1 Introduction of the Technique
85(1)
2 Basic Principle
86(1)
3 Protocol
86(1)
3.1 Reagents Required
86(1)
3.2 Working Solutions
87(1)
3.3 Protocol for DNA Isolation
87(1)
4 Precautions
87(1)
5 Applications
88(1)
References
88(1)
Isolation of DNA from Blood Samples by Salting Method
89(6)
1 Introduction
89(1)
2 Basic Principle
90(1)
3 Protocol
90(2)
3.1 Reagents Required
90(1)
3.2 Working Solutions
90(1)
3.3 Procedure for DNA Extraction
91(1)
4 Precautions
92(1)
5 Applications
93(2)
References
93(2)
CTAB or SDS-Based Isolation of Plant's DNA
95(12)
1 Introduction
95(1)
2 Basic Principle
96(2)
3 Protocol
98(5)
3.1 Reagents Required
98(1)
3.2 Working Solutions
98(1)
3.3 Procedure
99(4)
4 Precautions
103(1)
5 Applications
103(4)
References
105(2)
DNA Extraction by Spooling Method
107(4)
1 Introduction of the Technique
107(1)
2 Basic Principle
108(1)
3 Protocol
108(2)
3.1 Reagents Required
108(1)
3.2 Working Solutions
109(1)
3.3 Procedure
109(1)
4 Precautions HO
5 Applications
110(1)
References
110(1)
Magnetic Bead-Based Nucleic Acid Isolation
111(8)
1 Introduction
111(1)
2 Basic Principle
112(2)
3 Protocol
114(1)
3.1 Reagents Required
114(1)
3.2 Working Solution
114(1)
3.3 Procedure
114(1)
4 Precautions
115(1)
5 Applications
116(3)
References
116(3)
Density Gradient-Based Nucleic Acid Isolation
119(6)
1 Introduction of the Technique
119(1)
2 Basic Principle
120(1)
3 Protocol
121(2)
3.1 Reagents Required
121(1)
3.2 Working Solutions
121(1)
3.3 Protocol
122(1)
4 Precautions
123(1)
5 Applications
123(2)
References
123(2)
DNA Extraction from Agarose Gel Through Paper Strips
125(10)
1 Introduction
125(1)
2 Basic Principle
126(1)
3 Protocol
126(4)
3.1 Reagent Required
126(1)
3.2 Working Solutions
127(1)
3.3 Procedure
128(2)
4 Precautions
130(1)
5 Applications
130(5)
References
130(5)
Part III Major Applications of DNA and RNA
Transformation or Genetic Modification of Cells/Organisms
135(12)
1 Introduction
135(2)
2 Natural Method of Genetic Modification
137(1)
3 Genetic Modification in Plants
137(3)
4 Genetic Modification in Animals and Microbes
140(1)
5 Non-targeted Mutagenesis
141(1)
6 Genetic Engineering
141(6)
References
144(3)
Gene Cloning and Vectors
147(10)
1 Introduction
147(2)
2 Basic Steps of the Technique (Cell-Based DNA Cloning)
149(1)
3 Vector or Cloning Vector
150(1)
4 Types of Cloning Vectors
151(3)
5 Applications
154(3)
References
156(1)
Polymerase Chain Reaction (PCR)
157(8)
1 Concept of PCR
157(1)
2 Taq Polymerase
158(1)
3 Primers
159(1)
4 Buffer System
160(1)
5 The Reaction Conditions
160(2)
6 Basic Principle
162(1)
7 Short Protocol
162(1)
8 Applications
163(2)
References
163(2)
Southern and Northern Blotting
165(6)
1 Introduction
165(1)
2 Materials Required
166(1)
3 General Protocol
167(2)
3.1 Restriction Digestion and Gel Electrophoresis
167(1)
3.2 Transfer
167(1)
3.3 Prehybridization
168(1)
3.4 Hybridization
168(1)
3.5 Detection of Probe
169(1)
4 Application
169(2)
References
169(2)
Genome Mapping
171(8)
1 Introduction
171(1)
2 Difference Between Genome Mapping and DNA Sequencing
172(1)
3 Types of Genome Mapping
172(1)
4 Genetic Mapping
172(2)
5 Physical Mapping
174(5)
References
178(1)
Applications of DNA Sequencing Technologies for Current Research
179(13)
1 Introduction
179(1)
2 History
180(1)
3 Basic Principle
181(1)
4 Maxam-Gilbert Sequencing
181(1)
5 Chain-Termination or Dideoxy Method/Sanger Sequencing Principle
182(1)
6 Large-Scale Sequencing and De Novo Sequencing
183(1)
7 Shotgun Sequencing
183(1)
8 Clone Contig Approach
184(1)
9 Amplicon Sequencing
184(1)
10 High-Throughput Methods
185(1)
11 Long-Read Sequencing Methods: Nanopore Sequencing
185(1)
12 Short-Read Sequencing Methods: 454 Pyrosequencing
186(1)
13 Illumina (Solexa) Sequencing
187(1)
14 SOLiD Sequencing
188(1)
15 Ion Torrent Semiconductor Sequencing
188(1)
16 PacBio Sequencing
188(1)
17 The Basic Protocol for DNA Sequencing
189(1)
18 Precautions
190(1)
19 Need of DNA Sequencing Technology
191(1)
20 Conclusion
192(1)
References 192(5)
Index 197
Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).