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Embryo Models In Vitro: Methods and Protocols 2024 ed. [Hardback]

  • Formāts: Hardback, 323 pages, height x width: 254x178 mm, weight: 831 g, 3 Illustrations, black and white; XII, 323 p. 3 illus. With online files/update., 1 Hardback
  • Sērija : Methods in Molecular Biology 2767
  • Izdošanas datums: 07-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636855
  • ISBN-13: 9781071636855
  • Hardback
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  • Formāts: Hardback, 323 pages, height x width: 254x178 mm, weight: 831 g, 3 Illustrations, black and white; XII, 323 p. 3 illus. With online files/update., 1 Hardback
  • Sērija : Methods in Molecular Biology 2767
  • Izdošanas datums: 07-Feb-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071636855
  • ISBN-13: 9781071636855
This collection explores the use of pluripotent cells to generate early embryo-like structures in vitro, with the resultant 3D embryo-like structures in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Embryo Models In Vitro: Methods and Protocols serves as an ideal guide to these exciting and innovative advances in developmental biology.
Generation of Human Blastoids from Naļve Pluripotent Stem Cells.- Human
Pre-Gastrulation Embryo Culture in 3D Condition.- Protocol for the Generation
of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System.-
Generation of Human Trophoblast Stem Cell-Dependent Placental In Vitro
Models.- Workflow for Performing Genetic Manipulation in Human Trophoblast
Stem Cells Using CRISPR/Cas9 Technology.- Endometrial Assembloids to Model
Human Embryo Implantation In Vitro.- Stem Cell-Derived Microfluidic Amniotic
Sac Embryoid (µPASE).- Generation of 3D Trophoblast Organoids from Human
Naļve Pluripotent Stem Cells.- Induction of Human Extraembryonic Mesoderm
Cells from Naive Pluripotent Stem Cells.- Modeling Human Paraxial Mesoderm
Development with Pluripotent Stem Cells.- Generation of Stem Cell-Based Mouse
Embryo-Like Structures.- Embryonic Spinal Cord Innervation in Human Trunk
Organogenesis Gastruloids: Cardiac Versus Enteric Customization and Beyond.-
Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like
Structures from Mammalian Skin Dermal Fibroblasts.- Directed Differentiation
of Human Pluripotent Stem Cells to Cytotrophoblast and Syncytiotrophoblast.-
Single-Cell mRNA-sncRNA Co-Sequencing of Preimplantation Embryos.- Evaluation
of Stem-Cell Embryo Models by Integration with a Human Embryo Single-Cell
Transcriptome Atlas.- Inferring Gene Regulatory Networks and Predicting the
Effect of Gene Perturbations via IQCELL.- ShapeMetrics: A 3D Cell
Segmentation Pipeline for Single-Cell Spatial Morphometric Analysis.- Guinea
Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence.-
Manual Dissociation of Mammalian Preimplantation Embryos for Single-Cell
Genomics.- Whole-Mount RNA, Single Molecule RNA (smRNA), and DNA Fluorescence
In Situ Hybridization (FISH) in Mammalian Embryos.