Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal is to understand the energetic relationships between macromoleculer structures and biological functions.
This volume supplements Volumes 259 and Volume 295 of Methods in Enzymology.
Key Features
* Probing Stability of Helical Transmembrane Proteins
* Energetics of Vinca Alkaloid Interactions with Tubulin
* Deriving Complex Ligand Binding Formulas
* Mathematical Modeling of Cooperative Interactions in Hemoglobin
* Analysis of Interactions of Regulatory Protein TyrR with DNA
* Parsing Free Energy of Drug-DNA Interactions
* Use of Fluorescence as Thermodynamics Tool
Recenzijas
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Papildus informācija
Key Features * Probing Stability of Helical Transmembrane Proteins * Energetics of Vinca Alkaloid Interactions with Tubulin * Deriving Complex Ligand Binding Formulas * Mathematical Modeling of Cooperative Interactions in Hemoglobin * Analysis of Interactions of Regulatory Protein TyrR with DNA * Parsing Free Energy of Drug-DNA Interactions * Use of Fluorescence as Thermodynamics Tool
Contributors To Volume 323 xi Preface xv Volumes In Series xvii Quantitative Dissection of Transcriptional Control System: N-Dependent Antitermination Complex of Phage λ as Regulatory Paradigm 1(30) Marc R. Van Gilst Peter H. von Hippel Problems and Prospects in Microcalorimetry of Biological Macromolecules 31(32) George P. Privalov Peter L. Privalov Probing Stability of Helical Transmembrane Proteins 63(14) Karen G. Fleming Energetics of Vinca Alkaloid Interactions with Tubulin 77(27) Sharon Lobert John J. Correia Kinetics and Thermodynamics of Conformational Equilibria in Native Proteins by Hydrogen Exchange 104(20) Cammon B. Arrington Andrew D. Robertson Mathematical Modeling of Cooperative Interactions in Hemoglobin 124(31) Michael L. Johnson Deriving Complex Ligand-Binding Formulas 155(12) Michael L. Johnson Martin Straume Calculation of Entropy Changes in Biological Processes: Folding, Binding, and Oligomerization 167(10) L. Mario Amzel Evaluating Energetics of Erythropoietin Ligand Binding to Homodimerized Receptor Extracellular Domains 177(30) Preston Hensley Michael L. Doyle David G. Myszka Robert W. Woody Michael R. Brigham-Burke Connie L. Erickson-Miller Charles A. Griffin Christopher S. Jones Dean E. McNulty Shawan P. OBrien Bernard Y. Amegadzie Laurie MacKenzie M. Dominic Ryan Peter R. Young Measurement of Protein Interaction Bioenergetics: Application to Structural Variants of AntisCD4 Antibody 207(24) Michael L. Doyle Michael R. Brigham-Burke Michael N. Blackburn Ian S. Brooks Thomas M. Smith Roland Newman Mitchell Reff Walter F. Stafford III Raymond W. Sweet Alemseged Truneh Preston Hensley Daniel J. OShannessy Analysis of Interaction of Regulatory Protein TyrR with DNA 231(23) Geoffrey J. Howlett Barrie E. Davidson Proteolytic Footprinting Titrations for Estimating Ligand-Binding Constants and Detecting Pathways of Conformational Switching of Calmodulin 254(48) Madeline A. Shea Brenda R. Sorensen Susan Pedigo Amy S. Verhoeven Analysis of Reversibly Interacting Macromolecular Systems by Time Derivative Sedimentation Velocity 302(23) Walter F. Stafford Kinetic, Equilibrium, and Thermodynamic Analysis of Macromolecular Interactions with BIACORE 325(15) David G. Myszka Structure-Function Relationships in Two-Component Phospholipid Bilayers: Monte Carlo Simulation Approach Using a Two-State Model 340(33) Istvan P. Sugar Rodney L. Biltonen Parsing Free Energies of Drug-DNA Interactions 373(33) Ihtshamul Haq Terence C. Jenkins Babur Z. Chowdhry Jinsong Ren Jonathan B. Chaires Direct Measurement of Sodium Ion Release from DNA on Binding of Cationic Ligands 406(13) Charles H. Spink Thomas P. White Enthalpy-Entropy Compensations in Nucleic Acids: Contribution of Electrostriction and Structural Hydration 419(23) Luis A. Marky Donald W. Kupke Time-Resolved Fluorescence Methods for Analysis of DNA-Protein Interactions 442(17) David P. Millar Use of Fluorescence Spectroscopy as Thermodynamics Tool 459(14) Maurice R. Eftink Microsecond Dynamics of Biological Macromolecules 473(38) Joseph R. Lakowicz Ignacy Gryczynski Grzegorz Piszczek Leah Tolosa Rajesh Nair Michael L. Johnson Kazimierz Nowaczyk Author Index 511(20) Subject Index 531(14) Errata 545
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