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Epitope Mapping Protocols Fourth Edition 2025 [Hardback]

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  • Formāts: Hardback, 394 pages, height x width: 254x178 mm, 78 Illustrations, color; 6 Illustrations, black and white; XI, 394 p. 84 illus., 78 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2937
  • Izdošanas datums: 03-Jul-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071645900
  • ISBN-13: 9781071645901
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Epitope Mapping Protocols Fourth Edition 2025Palielināt
  • Formāts: Hardback, 394 pages, height x width: 254x178 mm, 78 Illustrations, color; 6 Illustrations, black and white; XI, 394 p. 84 illus., 78 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2937
  • Izdošanas datums: 03-Jul-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071645900
  • ISBN-13: 9781071645901
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071645901.html
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This detailed new edition explores methodologies for the characterization of antibody-antigen interactions tailored to different applications and requirements. Beginning with an overview of key concepts and definitions related to antibody epitopes, the book continues with sections on structural biology methods, display technologies to analyze libraries of proteins or protein arrays, techniques based on protein labeling, as well as computational approaches and binding assays that rely on binding interactions or competition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and up-to-date, Epitope Mapping Protocols, Fourth Edition serves as an ideal resource for scientists from a wide range of fields involved in critical antibody research.

 

Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

An Overview of Epitopes and Methods for Antibody Epitope Mapping.-
Antibody-Antigen Epitope Mapping by X-Ray Crystallography.- Mapping Antibody
Epitopes by Solution NMR Spectroscopy: Practical Considerations.-
Cryo-Electron Microscopy-Based Epitope Mapping.- Epitope Mapping Using Yeast
Display and Next Generation Sequencing.- Rapid Mapping of Protein Binding
Sites and Conformational Epitopes by Coupling Yeast Surface Display to
Chemical Labeling and Deep Sequencing.- High-Density Antigen Microarrays for
the Assessment of Antibody Selectivity.- Mammalian Cell Display with
Automated Oligo Design and Library Assembly Allows for Rapid Residue Level
Conformational Epitope Mapping.- Epitope Mapping of Antibodies Using
Bacterial Cell Surface Display of Gene Fragment Libraries.- Mapping of
Antibody Structural Epitopes Using Bacterial Cell Surface Display of Folded
Domains.- Highly Multiplexed Immunoassays for the Validation of Anti-GPCR
Antibodies.- Probing Antibody Binding Sites on G Protein-Coupled Receptors
Using Genetically-Encoded Photo-Activatable Cross-Linkers.- Genetic Code
Expansion to Enable Site-Specific Post-Translational Peptide Epitope Tagging
of G Protein-Coupled Receptors in Live Cells.- Multiplexed Epitope Mapping of
HIV-1 Neutralizing Antibodies by Coupling Chemical Labeling of Cysteines to
Deep Sequencing.- Predicting Epitopes of Synthetic Binding Proteins Using
Protein Structure Prediction and Protein-Protein Docking.- Structure-Based
Epitope Profiling with the Structural Profiling of Antibodies to Cluster by
Epitope 2 (SPACE2) Algorithm.- Binding Kinetics and Epitope Binning Using
Surface Plasmon Resonance.- High-Throughput Epitope Binning Analysis of Human
Monoclonal Antibodies to Assess Epitope Diversity Using the Octet Bio-Layer
Interferometry Platform.- Competitive Epitope Binning Using HT-SPR.-
Label-Free Cell-Based Assay for Characterization of Biomolecules and
Receptors.- B Cell Epitope Mapping by High Density Competition ELISAs.