This detailed volume presents protocols centered on the sensing and signaling of ethylene, a plant hormone with a vital role in fruit ripening, seed germination, stress adaptation, and a wide array of developmental processes. Beginning with a section on ethylene detection and quantification techniques, including gas chromatography and analysis during hypoxia and pathogen interactions, the book continues by examining the role of ethylene in post-harvest biology and fruit ripening, and associated regulatory mechanisms, including promoter characterization and protein-protein interactions. Further sections explore bioinformatics workflows for unraveling mechanistic roles of ethylene at the cellular and whole-plant levels, as well as the regulatory capacity of ethylene in plant developmental processes, root system modifications, and adaptation to diverse biotic and abiotic stressors. Written for the highly successful Methods in Molecular Biology series, chapters offer readers a hands-on approach to investigate the myriad roles that ethylene plays in plant growth and development.
Authoritative and practical, Ethylene Sensing and Signaling serves as a key resource for scientists seeking to employ reliable methodologies in understanding the central function of ethylene in plant biology.
