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E-grāmata: Extracellular Vesicles in Diagnosis and Therapy

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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2504
  • Izdošanas datums: 25-Apr-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071623411
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  • Formāts: PDF+DRM
  • Sērija : Methods in Molecular Biology 2504
  • Izdošanas datums: 25-Apr-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071623411
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This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Extracellular Vesicles in Diagnosis and Therapy serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication.
Aptasensors for Cancerous Exosome Detection.- Detection of
Cancer-Derived Exosomes Using a Sensitive Colorimetric Aptasensor.- Isolation
of Circulating Extracellular Vesicles by High-Performance Size-Exclusion
Chromatography.- Isolation and Proteomic Analysis of Mouse Serum Small
Extracellular Vesicles for Individual Subject Analysis.- Protocol for
Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with
Flow Cytometry.- Targeted Mass Spectrometry-Based Proteomics Method to
Quantify Placental Extracellular Vesicles.- Isolation and Characterization of
Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived
Cardiovascular Progenitor Cells.- Isolation and Characterization of Salivary
Exosomes for Cancer Biomarker Discovery.- Isolation and Characterization of
Urinary Extracellular Vesicles for MicroRNA Biomarker Signature Development
with Reference to MISEV Compliance.- Direct Detection of Extracellular
Vesicle miRNAs Using a Single-Step RT-qPCR Assay.- Purification and
Phosphoproteomic Analysis of Plasma-Derived Extracellular Vesicles.-
Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and
Serum.- Extracellular Vesicles and their Use as Vehicles of Immunogens.-
Isolation and Fluorescent Labeling of Extracellular Vesicles from Cultured
Tumor Cells.- Generation, Characterization, and Count of Fluorescent
Extracellular Vesicles.- Optimized Protocol for Plasma-Derived Extracellular
Vesicles Loading with Synthetic miRNA Mimic Using Electroporation.-
Extracellular Vesicle Loading via pH-Gradient Modification.