This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis.
This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.
Confocal microscopy in the life sciences; Imaging flow cytometry for
quantification of cellular parameters; Live-cell imaging: Seeing is
believing; Atomic force microscopy: A tool for imaging, manipulating
biomolecules, and quantifying single-molecule interactions in biological
systems; Confocal Raman microscopy: Imaging the chemistry; Tracking cancer:
In vivo imaging techniques; Quantum dots: Concepts, imaging, and therapeutic
applications in cancer; Selecting the appropriate in situ proximity ligation
assay protocol; Cell detection and joint shape tracking using local image
filters; Studying subcellular signaling events in living microglial cells
using fluorescence resonance energy transfer-based nanosensors; Membrane
trafficking under the microscope, what new imaging technologies have brought
to light; Illuminating the cycle of life; Methods for the visualization of
circadian rhythms: From molecules to organisms; Measurement of lysosomal ion
homeostasis by fluorescence microscopy; Capturing quantitative features of
protein expression from in situ fluorescence microscopic images of cancer
cell populations; Cancer cell invadopodia: Visualization and quantification
tools.
Raquel Seruca, MD, PhD, is a Professor at Medical Faculty of Porto, vice-president of Ipatimup and Coordinator of the Cancer research program at i3s, both located in Porto, Portugal. Dr. Seruca is an expert in oncobiology with a specific interest in the role of adhesion molecules in cancer and molecular genetics of gastrointestinal cancer. She has more than 200 publications in international peer-review journals, with an h-index of 52, and several national and international awards by her relevant contributions for science and society.
Jasjit S. Suri, PhD, MBA, Fellow AIMBE, is an innovator, visionary scientist, and an internationally known world leader. Dr. Suri was crowned with the Director Generals Gold medal in 1980 and elected as a Fellow of American Institute of Medical and Biological Engineering (AIMBE), awarded by National Academy of Sciences in Washington DC in 2004. Dr. Suri has held executive positions, been the Chairman of the IEEE Denver section, and won over 50 awards during his career.
Joćo M. Sanches, PhD, is a Professor at the Bioengineering Department (DBE) of the Instituto Superior Técnico (IST), Univers. of Lisbon, and Member of the board of directors of the Institute for Systems and Robotics (ISR/IST). Professor Sanches has about 150 international publications in Biomedical Engineering in statistical signal and image processing methods for biological and medical diagnosis. He is senior member of the IEEE Engineering in Medicine and Biology Society and Member of the Bio Imaging and Signal Processing Technical Committee (BISP-TC) of the IEEE Signal Processing Society.
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