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E-grāmata: Food Immunoassay

  • Formāts: EPUB+DRM
  • Izdošanas datums: 13-Nov-2019
  • Izdevniecība: Springer Verlag, Singapore
  • Valoda: eng
  • ISBN-13: 9789811390340
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  • Formāts: EPUB+DRM
  • Izdošanas datums: 13-Nov-2019
  • Izdevniecība: Springer Verlag, Singapore
  • Valoda: eng
  • ISBN-13: 9789811390340

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This book systematically covers immunoassays for food, presenting detailed approaches such as antigen design, food matrix pre-treatment and detection format optimization for 9 classes of food hazards and nutrition constituents. Offering ideas on how to improve the efficiency of recognized xenobiotics and food contents, this practical book also describes the discovery and utilization of novel immune agents like aptamer and molecular imprinted polymers in food analysis. It is intended for a broad range of areas, including biologists and food chemists, and is sure to become a key reference resource for students and professionals alike.

1 Introduction of Immunoassays
1(14)
1.1 Overview
1(1)
1.2 ELISA
1(10)
1.2.1 Principle of ELISA
2(1)
1.2.2 Classification of ELISA
2(1)
1.2.3 Preparation of mAb
3(4)
1.2.4 Establishment of Indirect and Competitive ELISA
7(4)
1.3 Development of Strip Test
11(3)
1.3.1 Principle of Strip Test
11(1)
1.3.2 Steps for Strip Test
12(2)
References
14(1)
2 Mycotoxin Immunoassay in Food
15(38)
2.1 Overview
15(16)
2.1.1 Aflatoxins
15(3)
2.1.2 Ochratoxin
18(1)
2.1.3 Fumonisins
19(1)
2.1.4 Trichothecenes
20(3)
2.1.5 Zearalenone
23(1)
2.1.6 Sterigmatocystin
24(2)
2.1.7 Citrinin
26(1)
2.1.8 Alternaria Toxins
27(1)
2.1.9 Patulin
27(2)
2.1.10 Other Mycotoxin
29(2)
2.2 Design and Synthesis of Antigen
31(10)
2.2.1 Aflatoxins
31(1)
2.2.2 Ochratoxin
32(1)
2.2.3 Fumonisins
33(1)
2.2.4 Trichothecenes
33(2)
2.2.5 Zearalenone
35(1)
2.2.6 Sterigmatocystin
36(1)
2.2.7 Citrinin
37(1)
2.2.8 Alternaria Toxins
38(1)
2.2.9 Patulin
39(1)
2.2.10 Other Mycotoxin
39(2)
2.3 ELISA
41(7)
2.3.1 Anatoxins
42(1)
2.3.2 Ochratoxin
43(1)
2.3.3 Fumonisins
44(1)
2.3.4 Trichothecenes
44(2)
2.3.5 Zearalenone
46(1)
2.3.6 Sterigmatocystin
46(1)
2.3.7 Citrinin
47(1)
2.3.8 Alternaria Toxins
47(1)
2.3.9 Patulin
48(1)
2.4 Colloidal Gold Immunochromatographic Strips
48(1)
2.5 Perspectives
49(1)
References
50(3)
3 Heavy Metal Immunoassay in Food
53(16)
3.1 Overview
53(5)
3.2 Design and Synthesis of Antigen
58(4)
3.2.1 Lead
58(1)
3.2.2 Cadmium
59(1)
3.2.3 Mercury
60(1)
3.2.4 Chromium
61(1)
3.2.5 Copper
62(1)
3.3 Enzyme-Linked Immunosorbent Assay
62(2)
3.4 Colloidal Gold Lateral Flow Assay
64(1)
3.5 Perspectives
65(1)
References
66(3)
4 Pesticide Immunoassay in Food
69(86)
4.1 Pesticide Overview
69(25)
4.1.1 Pyrethroid Insecticides
69(3)
4.1.2 Organic Phosphorus Insecticide
72(2)
4.1.3 Neonicotinoid Insecticides
74(1)
4.1.4 Triazole Fungicides
74(3)
4.1.5 Strobilurin Fungicides
77(3)
4.1.6 Anilinopyrimidine Fungicides
80(1)
4.1.7 Amide Fungicides
80(3)
4.1.8 Herbicide Pesticides
83(6)
4.1.9 Other Pesticides
89(5)
4.2 Design and Synthesis of Antigen (Hapten)
94(33)
4.2.1 Design and Synthesis of Pyrethroid Antigens
95(2)
4.2.2 Design and Synthesis of Organophosphorus Insecticides Antigen
97(4)
4.2.3 Design and Synthesis of Neonicotinoid Insecticides Antigen
101(1)
4.2.4 Design and Synthesis of Triazole Fungicides Antigen
102(3)
4.2.5 Design and Synthesis of Strobilurin Fungicides Antigen
105(3)
4.2.6 Design and Synthesis of Anilinopyrimidine Fungicides Haptens
108(7)
4.2.7 Design and Synthesis of Trifloxystrobin Antigen Amide Fungicides
115(5)
4.2.8 Design and Synthesis of Bensulfuron-Methyl Herbicide Antigen
120(1)
4.2.9 Other Pesticides
121(6)
4.3 Enzyme-Linked Immunosorbent Assay
127(20)
4.3.1 ELISA for Pyrethroids Analysis
127(2)
4.3.2 ELISA of Organophosphorus Pesticides
129(2)
4.3.3 ELISA of Neonicotinoid Insecticides
131(2)
4.3.4 ELISA of Triazole Fungicides
133(1)
4.3.5 ELISA of Strobilurin Fungicides
134(4)
4.3.6 ELISA of Anilinopyrimidine Fungicides
138(2)
4.3.7 ELISA of Amide Fungicides
140(2)
4.3.8 ELISA of Herbicide Pesticides
142(1)
4.3.9 ELISA of Other Pesticides
143(4)
4.4 Strip Test
147(1)
4.5 Perspectives
148(2)
References
150(5)
5 β-Agonist and Hormone Immunoassays in Food
155(22)
5.1 Overview
155(3)
5.2 Design and Synthesis of Antigen
158(11)
5.2.1 0-Agonists
158(6)
5.2.2 Artificial Hormone
164(5)
5.3 Enzyme-Linked Immunosorbent Assay
169(2)
5.3.1 Sample Pretreatment
169(1)
5.3.2 Enzyme-Linked Immunosorbent Assay (ELISA)
170(1)
5.4 Colloidal Gold Immunochromatographic Strips
171(2)
5.5 Perspectives
173(1)
References
173(4)
6 Antibiotics Immunoassay in Food
177(56)
6.1 Aminoglycoside Antibiotics
177(6)
6.1.1 Overview
177(3)
6.1.2 Synthesis of Antigen and Immunoassay
180(1)
6.1.3 Immunochromatographic Test Strip (ICTS)
181(2)
6.2 Amphenicols Antibiotics
183(3)
6.2.1 Overview
183(1)
6.2.2 Synthesis of Antigen and Immunoassay
184(1)
6.2.3 Immunochromatographic Test Strip (ICTS)
185(1)
6.3 β-Lactam Antibiotics
186(7)
6.3.1 Overview
186(4)
6.3.2 Synthesis of Antigen and Immunoassay
190(2)
6.3.3 Immunochromatographic Test Strip (ICTS)
192(1)
6.4 Nitrofuran Antibiotics
193(4)
6.4.1 Overview
193(1)
6.4.2 Immunoassay for Nitrofurans
194(2)
6.4.3 Immunochromatographic Test Strip (ICTS)
196(1)
6.5 Quinolones Antibiotics
197(8)
6.5.1 Overview
197(1)
6.5.2 Synthesis of Antigen and Immunoassay for QNs
198(6)
6.5.3 Immunochromatographic Test Strip
204(1)
6.6 Sulfonamides Antibiotics
205(8)
6.6.1 Overview
205(2)
6.6.2 Synthesis of Antigen and Immunoassay for SAs
207(3)
6.6.3 Immunochromatographic Test Strip for SAs
210(3)
6.7 Tetracyclines Antibiotics
213(7)
6.7.1 Overview
213(1)
6.7.2 Synthesis of Antigen and Immunoassay for TCs
213(3)
6.7.3 Immunochromatographic Test Strip for TCs
216(4)
6.8 Others
220(6)
6.8.1 Macrolide Antibiotics
220(2)
6.8.2 Lincomycin Antibiotics
222(2)
6.8.3 Nitroimidazoles
224(1)
6.8.4 Polypeptide Antibiotics
225(1)
6.9 Perspectives
226(1)
References
227(6)
7 Illegal Additives Immunoassay in Food
233(22)
7.1 Overview
233(4)
7.1.1 Illegal Colors
234(1)
7.1.2 Malachite Green
235(1)
7.1.3 Phthalate Esters
235(1)
7.1.4 Melamine
236(1)
7.2 Design and Synthesis of Antigen
237(7)
7.2.1 Synthesis of Sudan I Antigen
237(2)
7.2.2 Synthesis of Rhodamine B (RB) Antigen
239(1)
7.2.3 Synthesis of Tartrazine Antigen
239(2)
7.2.4 Synthesis of Malachite Green (MG) Antigen
241(1)
7.2.5 Synthesis of PAEs Antigen
242(1)
7.2.6 Synthesis of MEL Antigen
243(1)
7.3 ELISA
244(5)
7.3.1 Illegal Colors
245(1)
7.3.2 Malachite Green
246(1)
7.3.3 PAEs
246(2)
7.3.4 Mel
248(1)
7.4 Immunochromatographic Strip (ICS)
249(1)
7.5 Perspective
250(1)
References
251(4)
8 Pathogen Immunoassay in Food
255(66)
8.1 Overview of Foodborne Pathogens
255(8)
8.1.1 Salmonella spp
255(1)
8.1.2 Listeria Monocytogenes
256(1)
8.1.3 Escherichia Coli (0157:H7)
256(1)
8.1.4 Staphylococcus Aureus and Staphylococcal Enterotoxins
257(1)
8.1.5 Vibrio Parahemolyticus
258(3)
8.1.6 Cronobacter spp
261(1)
8.1.7 Campylobacter jejuni
262(1)
8.1.8 Clostridium botulinum and Botulinum Toxin
262(1)
8.1.9 Norovirus
263(1)
8.2 Surface Antigens of the Foodborne Pathogens
263(3)
8.2.1 Whole Cell Antigen
263(1)
8.2.2 Surface Proteins
264(1)
8.2.3 Peptide
265(1)
8.2.4 Polysaccharide
265(1)
8.3 Immunogens and Antibodies of the Foodborne Pathogens
266(16)
8.3.1 Salmonella spp
266(1)
8.3.2 Listeria Monocytogenes
267(3)
8.3.3 Escherichia Coli (0157:H7)
270(1)
8.3.4 Staphylococcus Aureus and Staphylococcal Enterotoxins
271(1)
8.3.5 Vibrio. Parahemolyticus
271(1)
8.3.6 Cronobacter spp
272(2)
8.3.7 Campylobacter Jejuni
274(2)
8.3.8 Clostridium botulinum and Botulinum Toxin
276(1)
8.3.9 Norovirus
277(5)
8.4 ELISA Methods of the Foodborne Pathogens
282(11)
8.4.1 Salmonella spp
282(1)
8.4.2 Listeria Monocytogenes
283(2)
8.4.3 Escherichia Coli (0157:H7)
285(1)
8.4.4 Staphylococcus Aureus and Staphylococcal Enterotoxins
285(1)
8.4.5 Vibrio. Parahemolyticus
286(2)
8.4.6 Cronobacter spp
288(1)
8.4.7 Campylobacter Jejuni
288(2)
8.4.8 Clostridium botulinum and Botulinum Toxin
290(2)
8.4.9 Norovirus
292(1)
8.5 Immunochromatographic Strips of the Foodborne Pathogens
293(17)
8.5.1 Salmonella spp
293(3)
8.5.2 Listeria Monocytogenes
296(2)
8.5.3 Escherichia Coli (0157:H7)
298(1)
8.5.4 Staphylococcus Aureus and Staphylococcal Enterotoxins
299(2)
8.5.5 Vibrio Parahemolyticus
301(3)
8.5.6 Cronobacter spp
304(1)
8.5.7 Campylobacter Jejuni
305(1)
8.5.8 Clostridium botulinum and Botulinum Toxin
306(3)
8.5.9 Norovirus
309(1)
8.6 Perspectives
310(1)
References
311(10)
9 Immunoassay for Allergens in Food
321(18)
9.1 Overview
321(5)
9.1.1 Allergic Reaction
321(1)
9.1.2 Food Allergens
322(4)
9.2 ELISA for Food Allergens
326(6)
9.2.1 Glycinin in Soybean
326(2)
9.2.2 Prolamins
328(1)
9.2.3 Casein
329(1)
9.2.4 Arah 1
329(1)
9.2.5 Ovalbumin
330(1)
9.2.6 Parvalbumin
331(1)
9.2.7 Crustacean Tropomyosin
331(1)
9.3 Colloidal Gold Immunochromatographic Strip Test
332(2)
9.3.1 Strip Test for j6-Conglycinin
333(1)
9.3.2 Arah 2
334(1)
9.4 Perspective
334(1)
References
335(4)
10 Food Nutrition Immunoassay
339
10.1 Overview
339(3)
10.1.1 Vitamin B2
339(1)
10.1.2 Biotin
340(1)
10.1.3 Folic Acid
341(1)
10.1.4 Vitamin Bi2
341(1)
10.2 Design and Synthesis of Antigen
342(4)
10.2.1 Vitamin B2
342(1)
10.2.2 Biotin
343(1)
10.2.3 Folic Acid
344(1)
10.2.4 Vitamin B12
345(1)
10.3 Enzyme-Linked Immunosorbent Assay (ELISA)
346(8)
10.3.1 Vitamin B2
346(3)
10.3.2 Biotin
349(1)
10.3.3 Folic Acid
350(3)
10.3.4 Vitamin B12
353(1)
10.4 Colloidal Gold Immunochromatographic (ICT) Strips
354(7)
10.4.1 Vitamin B2
354(3)
10.4.2 Biotin
357(1)
10.4.3 Folic Acid
358(1)
10.4.4 Vitamin B12
359(2)
10.5 Perspectives
361(1)
References
361
Chuanlai Xu is a Full Professor at the International Joint Research Laboratory for Biointerface and Biodetection, and State Key Laboratory of Food Science and Technology, Jiangnan University in China. He obtained his doctorate from Jiangnan University in 2002, and was a Visiting Scholar at the University of East Anglia (2007) and the University of Michigan (2008). For almost 20 years his research has focused on fast detection technology and measures, and to date he has developed more than 400 monoclonal antibodies against mycotoxins, antibiotics, pesticides, and hormones. Over 80% fabricated fast reagents and devices, such as enzyme-linked immunoassay, immunoaffinity materials and strip sensors have been widely used in the food industry and in-field detection. He has published over 300 peer-reviewed articles and holds over 200 patents. He is the EIC of Food and Agricultural Immunology.

Hua Kuang is a Full Professor at State Key Laboratory of Food Science and Technology, Jiangnan University in China. She received her BS degree (2003) from Jiangnan University, and her MS (2006) and PhD (2009) degrees from the China Agricultural University. She was a postdoctoral fellow (20122013) at the City University of Hong Kong. She has mainly focused on the plasmonic circular dichroism properties of nanoscale self-assemblies and fabrication of detection probes for biological applications. She has published more than 100 peer-reviewed journal articles, and has received various awards, including from the Excellent Youth Foundation of Jiangsu Scientific Committee (2014), Young top-notch talent (2014), Excellent Youth Foundation (Ministry of Science & Technology) (2015), Cheung Kong Young Scholar (Ministry of Education of China) (2015), Youth Science and technology innovation Pioneer (Ministry of Science & Technology) (2016) and APEC Science Prize for Innovation, Research and Education (Asia-Pacific Economic Cooperation) (2016). She is an associate editor of Science Bulletin.

Liguang Xu is a Full Professor at the School of Food Science and Technology at Jiangnan University, China. He received his BS degree (2006) in Food Science from Qingdao Agricultural University, and his MS (2009) in Food Science and PhD (2012) in Biosensors from Jiangnan University. His PhD research focused on the surface-enhanced Raman scattering and circular dichroism properties of nanoparticle diverse superstructures. He was a Postdoctoral Fellow (20152016) at the University of Michigan, where he cooperated with Prof. Nicolas A. Kotov, studying the application of plasmonic circular dichroism for sensing DNA fragments of resistant bacteria in blood. His current interests include design and synthesis of nanoparticle assembled superstructures applied in biological systems.