This book is designed to give students an understanding of the role of microorganisms in food processing and preservation; the relation of microorganisms to food spoilage, foodborne illness, and intoxication; general food processing and quality control; the role of microorganisms in health promotion; and federal food processing regulations. The listed laboratory exercises are aimed to provide a hands-on-opportunity for the student to practice and observe the principles of food microbiology. Students will be able to familiarize themselves with the techniques used to research, regulate, prevent, and control the microorganisms in food and understand the function of beneficial microorganism during food manufacturing process.
The second edition add 5 new chapters including Chapter 10 -Thermal inactivation of Escherichia coli O157:H7 in mechanically tenderized beef steaks and color measurements, Chapter 11-Evaluate antimicrobial activity of chlorine water on apples and measurement of free chlorine concentrations, Chapter 12-Evaluate cross-contamination of Salmonella on tomatoes in wash water using most probable number (MPN) technique, Chapter 15-DNA extraction and purity determination of foodborne pathogens, and Chapter 16-Practice of multiplex PCR to identify bacteria in bacterial solutions. It also includes new lab work flowcharts for Gram-staining and endospore-staining technology in Chapter 1, pour plating and spread plating in Chapter 3, Enterotube II in Chapter 9, and Kirby Beau test procedure in Chapter 20. It includes a new sample of syllabus with the hybrid teaching of both lecture and lab sections in one course, which will assist junior faculty/instructors to develop similar lecture and lab courses.
Food Microbiology Laboratory Safety and Notebook Record.- Staining
Technology and Bright-field Microscope Use.- Enumeration of Bacteria in Broth
Suspension by Spread and Pour Plating.- Isolation of Foodborne pathogens
on Selective, Differentiate and Enrich Medium by Streak-plating.- Enumeration
of Aerobic plate counts, Coliforms and Escherichia coli of Organic Fruit
Juice on Petri-film.- Enumeration and identification of Staphylococcus aureus
in Chicken Salads.- Enumeration and identification Listeria monocytogenes
on ready-to-eat (RTE) frankfurters.- Isolation and identification of
Salmonella and Campylobacter spp. on broiler carcasses.- Thermal inactivation
of Escherichia coli O157:H7 in Non-intact reconstructed beef
patties.- Thermal inactivation of Escherichia coli O157:H7 in mechanically
tenderized beef steaks and color measurements.- Evaluate antimicrobial
activity of chlorine water on apples and measurement of free chlorine
concentrations.- Evaluate cross-contamination of Salmonella on tomatoes in
wash water using most probable number (MPN) technique.- Cultivation of
Anaerobic Bacteria in Canned Food.- Observation and enumeration of Molds from
Spoiled Bread.- DNA Extraction and Purity Determination of Foodborne
Pathogens.- Practice of Multiplex PCR to identify bacteria in bacterial
solutions.- PCR Identification of Listeria monocytogenes in Deli
Meat.- Cheese Making and Characterization.- Wine and Sauerkraut Making and
Characterization.- Introduction of oral presentation and job interview
preparation.- Appendix- Samples of Food Microbiology Lab Course Syllabus.
Dr. Cangliang Shen is an Associate Professor/Extension Specialist at Davis College, Division of Animal & Nutritional Sciences and Family & Community Development, West Virginia University. Dr. Shen worked at IEH Consulting Groups (Research Associate), USDA-ARS (Postdoctoral Research Microbiologist), and Western Kentucky University (Assistant Professor) after obtaining his Ph.D. from Colorado State University. Dr. Shen is currently teaching General Microbiology, Food Microbiology Lab, and Food Chemistry courses. Dr. Shens research interest is in developing postharvest sanitizing procedures for reducing microbial food safety risks on poultry meat products and fresh produce. Dr. Shen has published 49 peer-review journal articles, 2 textbooks, and 2 book chapters, and secured USDA-NIFA grants over $3.0 million. Dr. Shen serves as the editorial board member of Journal of Food Protection and Food Protection Trends. Dr. Shen received Outstanding Volunteer Award of Food Microbiology Division-Institute of Food Technologists (IFT) in 2018. He was the Chair of Food Microbiology Division-IFT in 2019-2020 and he is the Chair of Biotechnology Division-IFT in 2022-2023.
Dr. Yifan Zhang is a Professor in the Department of Nutrition and Food Science at Wayne State University, Michigan. She is an Editorial Board member of Journal of Food Protection, and a member of International Association of Food Protection (IAFP), Institute of Food Technologists (IFT), and American Society for Microbiology (ASM). Prior to joining the Wayne State faculty, Dr. Zhang has worked as a Postdoctoral Researcher at The Ohio State University. Dr. Zhangs research is on molecular characterization and risk analysis of foodborne bacteria, food and agricultural reservoir of antimicrobial resistance, urban agriculture, and the development of novel food safety control. Dr. Zhang is currently teaching Introductory Food Science and Advanced Food Science at the undergraduate level and Microbial Food Safety at the graduate level.
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