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E-grāmata: Foodborne Bacterial Pathogens: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2852
  • Izdošanas datums: 05-Sep-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071641002
  • Formāts - EPUB+DRM
  • Cena: 190,34 €*
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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2852
  • Izdošanas datums: 05-Sep-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071641002

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This updated volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Foodborne Bacterial Pathogens: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
 Direct or DNA extraction-free amplification and quantification of
foodborne pathogens.- The use of multiplex real time PCR for the simultaneous
detection of foodborne bacterial pathogens.- Viability detection of foodborne
bacterial pathogens in food environment by PMA-qPCR and by microscopy
observation.- Electrochemical Detection of Nucleic Acids using 3D Graphene
Screen Printed Electrodes.- Application of MinION long read sequencer for
semi-targeted detection of foodborne pathogens.- Integrating MALDI-TOF mass
spectrometry with machine learning techniques for rapid antimicrobial
resistances screening of foodborne bacterial pathogens.- Assessment of
viability of Listeria monocytogenes by flow cytometry. - Method to study the
survival abilities of foodborne bacterial pathogens under food processing
conditions.- Characterization of bacterial membrane fatty acid profiles for
biofilm cells.- Comparing gene expression between planktonic and biofilm
cells of foodborne bacterial pathogens through RT-qPC,- Characterization of
Food-Borne Pathogens in Biofilms: A 4D Structural Dynamics Approach Using
HCS-CLSM.- Zebrafish larvae microinjection and automated fluorescence
microscopy for studying Klebsiella pneumoniae infection and the host immune
response.- Flow Cytometry and Automated Microscopy Integration for Phagocytic
Analysis of hypervirulent Klebsiella pneumoniae in Dictyostelium
discoideum.- A Comprehensive Guide to Quality Assessment and Data Submission
for Genomic Surveillance of Enteric Pathogens.- Genomic pipeline for analysis
of mutational events in bacteria.- Using GWAS and Machine Learning to
identify and predict genetic variants associated with foodborne bacteria
phenotypic traits.- Identifying putative biomarkers of foodborne pathogens
using a metabolomic approach.- Illumina MiSeq 16S rRNA Gene Library
Preparation for Poultry Processing Microbiome Analyses.- Bioinformatic
pipeline for profiling foodborne bacterial ecology and resistome from
short-read metagenomics.