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Forensic DNA Analysis: Methods and Protocols 2023 ed. [Mīkstie vāki]

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  • Formāts: Paperback / softback, 431 pages, height x width: 254x178 mm, 92 Illustrations, color; 3 Illustrations, black and white; IX, 431 p. 95 illus., 92 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2685
  • Izdošanas datums: 15-Jul-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071632973
  • ISBN-13: 9781071632970
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Forensic DNA Analysis: Methods and Protocols 2023 ed.Palielināt
  • Formāts: Paperback / softback, 431 pages, height x width: 254x178 mm, 92 Illustrations, color; 3 Illustrations, black and white; IX, 431 p. 95 illus., 92 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2685
  • Izdošanas datums: 15-Jul-2024
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071632973
  • ISBN-13: 9781071632970
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781071632970.html
Keywords:

This volume focuses on the latest techniques used in forensic DNA analysis. The chapters include a comprehensive collection of extraction, quantification, STR amplification, and detection methods for routine forensic samples, including manual, semi-automated, and automated procedures using both home-brew and commercial products. The chapters also discuss probabilistic modeling software and specialized start-to-finish procedures for mitochondrial DNA analysis, archived latent fingerprints, latent DNA, rapid DNA profiling, and next-generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Forensic DNA Analysis: Methods and Protocols is a valuable resource for researchers interested in learning more about forensic DNA analysis procedures.


Forensic DNA Analysis: An Overview of the Laboratory Process.- Organic
Extraction of Nucleic Acids using Ethanol Precipitation or Microcon®
Centrifugal Filter Purification Methods.- Manual Silica-Based DNA
Extractions.- Applied Biosystems PrepFiler Forensic DNA Extraction Kit
(Manual and Semi-Automated via AutoMate Express).- Robotic DNA Extraction
Utilizing Qiagen BioSprint® 96 Workstation.- DNA Extraction of Bone through
Demineralization.- Differential Extraction with Purification via
Organic/Microcon® and Promega DNA IQ Methods.- DNA Purification from
Bloodstains and Buccal Cells/Saliva on FTA® Cards.- Yield Gel via
Quantitative Gel Electrophoresis.- Quantitative PCR of Alu Repeats using
PowerUp SYBR® Green Master Mix.- Quantitation of DNA using the Applied
Biosystems Quantifiler® Trio DNA Quantification Kit.- QIAGEN's Investigator®
Quantiplex Pro Kit.- DNA Amplification using Promega's PowerPlex® Fusion
Systems (5C and 6C).- Amplification of Extracted DNA and Direct Amplification
with the PowerPlex® Y23 System.- Applied Biosystems' GlobalFiler PCR
Amplification Kit.- QIAGENs Investigator® 24plex QS and GO! PCR
Amplification.- Low Volume STR Amplification Options: Coupling with Standard
or Fast PCR, Traditional or Normalized DNA Extraction, and/or Traditional or
Alternative Capillary Electrophoresis.- Capillary Electrophoresis with
Applied Biosystems' 3500 Genetic Analyzer.- Likelihood Ratio Calculation
using LRmix Studio.- Mitochondrial DNA Analysis.- An Optimized Forensic DNA
Analysis Workflow for Obtaining STR Results from Archived Latent
Fingerprints.- Detection of Latent DNA using a DNA Binding Dye.- Rapid DNA
Profile Development with Applied Biosystems RapidHIT ID
System.- Next-Generation Sequencing: ForenSeq DNA Signature Prep Kit with
the Illumina MiSeq FGx.