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Forensic DNA Typing Protocols Softcover reprint of the original 2nd ed. 2016 [Mīkstie vāki]

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  • Formāts: Paperback / softback, 298 pages, height x width: 254x178 mm, weight: 5815 g, 18 Illustrations, color; 10 Illustrations, black and white; X, 298 p. 28 illus., 18 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 1420
  • Izdošanas datums: 26-May-2018
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493980890
  • ISBN-13: 9781493980895
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Forensic DNA Typing Protocols Softcover reprint of the original 2nd ed. 2016Palielināt
  • Formāts: Paperback / softback, 298 pages, height x width: 254x178 mm, weight: 5815 g, 18 Illustrations, color; 10 Illustrations, black and white; X, 298 p. 28 illus., 18 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 1420
  • Izdošanas datums: 26-May-2018
  • Izdevniecība: Humana Press Inc.
  • ISBN-10: 1493980890
  • ISBN-13: 9781493980895
Citas grāmatas par šo tēmu:
Permanent link: https://www.kriso.lv/db/9781493980895.html

This volume presents a series of protocols and methods, some of which are not widely used by researchers/practitioners, and will aid in the execution of different laboratory techniques. Forensic DNA Typing Protocols, Second Edition is arranged into a series of related chapters. Chapter 1-3 examines two different aspects of RNA analysis for body fluid identification. Chapters 4-7 focuses on the storage of biological materials and the extraction of DNA from hard tissues. Chapters 8-10 present methods for monitoring the quality of DNA extracts, and steps to aid in the purification of DNA. Chapters 11-16 talk about methods on non-standard markers, such as INDELs, Y chromosome STRs, and mitochondrial DNA. Detailed procedures and data analysis for phenotypes and ancestry are explored in Chapter 17-19. The last chapter (20) looks at the application of DNA typing to the identification of non-human material to species level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Forensic DNA Typing Protocols, Second Edition, is a valuable resource for forensic specialists, researchers, and anyone interested in the field of forensic science.

 

Recenzijas

This is an instructional book that raises awareness of the technology available in genetic forensic sciences. The book is written in a way that is easy to understand for advanced professionals as well as beginners. Anyone wanting to understand the technology involved in forensic DNA analysis would benefit from this unique book. The value of this book lies in the simple and logical way it presents complex information in forensic medicine. (Luis F. Escobar, Doody's Book Reviews, November, 2016)

Collection of Samples for DNA Analysis.- Body Fluid Identification Using
mRNA Profiling.- mRNA Profiling for Vaginal Fluid and Menstrual Blood
Identification.- Preservation of and DNA Extraction from Muscle Tissue.- DNA
Extraction: Organic and Solid-Phase.- Extraction of DNA from Skeletal
Remains.- Extraction of DNA from Human Skeletal Material.- The Development
and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for
Forensic DNA Analysis.- NucleoSpin ®XS Columns for DNA Concentration and
Clean-Up.- Purification of PCR Products to Improve STR Profiles.- Analysis of
30 Biallelic INDEL Markers Using the Investigator DIPplex ® Kit.- Analysis of
Mitochondrial Control Region Using Sanger Sequencing.- Whole Human
Mitochondrial DNA Sequencing.- In-Solution Hybridization for the Targeted
Enrichment of the Whole Mitochondrial Genome.- Enhanced DNA Profiling of the
Semen Donor in Late Reported Sexual Assaults: Use of Y-Chromosome Targeted
Pre-Amplification and Next Generation Y-STR Amplification Systems.- Analysis
of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y STRs).- A
Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and
Hair Color from DNA.- Inference of Ancestry in Forensic Analysis I: Autosomal
Ancestry Informative Marker Sets.- Inference of Ancestry in Forensic
Analysis II: Analysis of Genetic Data.- Species Determination: The Role and
Use of the Cytochrome b Gene.