E-grāmata: FOXO Transcription Factors: Methods and Protocols

FOXO Transcription Factors: A Brief Overview.- Phosphorylation of FOXO
Proteins as a Key Mechanism to Regulate Their Activity.- Identification of
DAF-16/FOXO Binding Partners in Caenorhabditis elegans by Immunoprecipitation
and Mass Spectrometry.- Measuring FOXO Activity by Using qPCR-Based
Expression Analysis of FOXO Target Genes.-   Reporter Gene Assays to Measure
FOXO-Specific Transcriptional Activity.- Functional Motif Discovery in FOXO1
through CRISPR/Cas9 Exon Tiling Scan.- Cardiomyocyte-Specific FoxO1 Knockout
Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental
Considerations using Cre-loxP.- Methods to Study Structure and Dynamics of
FOXO Proteins.- Fluorescence Polarization Method to Assess the FOXO
Phosphopeptide Interaction with 14-3-3.- Characterization of FOXO3-14-3-3
Interaction by Isothermal Titration Calorimetry.- Identification of
Small-Molecule Modulators of FOXO3 through Virtual Screening.- Measuring FOXO
Nuclear Shuttling Dynamics by Fluorescence Microscopy.- Fluorescent Tagging
of Endogenous FOXO for Live Imaging and Pull-Down Assays.- Use of
Microfluidics for Study of FOXO3 Translocation Dynamics.- Multiplexed
Dual-Color Fluorescence-Based Distinction between Nuclear Trapping and
Translocation of FOXO3.- Immunocytochemistry-Based Detection of FOXO Isoforms
in Human Cancer and Fibroblasts.- Cell Death Regulation by Smed-foxO in the
Planarian Schmidtea mediterranea.- Characterizing the Role of daf-16/ C.
elegans FOXO in Lifespan and Healthspan.