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Geminiviruses: Methods and Protocols [Hardback]

  • Formāts: Hardback, 252 pages, height x width: 254x178 mm, 41 Illustrations, color; 19 Illustrations, black and white; XI, 252 p. 60 illus., 41 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2912
  • Izdošanas datums: 11-Mar-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 107164453X
  • ISBN-13: 9781071644539
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  • Hardback
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  • Formāts: Hardback, 252 pages, height x width: 254x178 mm, 41 Illustrations, color; 19 Illustrations, black and white; XI, 252 p. 60 illus., 41 illus. in color., 1 Hardback
  • Sērija : Methods in Molecular Biology 2912
  • Izdošanas datums: 11-Mar-2025
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 107164453X
  • ISBN-13: 9781071644539
Citas grāmatas par šo tēmu:
This book provides a comprehensive list of the methods used in basic and applied geminivirus research. Chapters guide readers through foundational methods, advanced techniques in cloning, sequencing, phylogenetic, recombination analysis, diagnostics,  and virus-host interaction studies. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Geminiviruses: Methods and Protocols aims to be a practical laboratory guide and serve as a valuable "one-stop-shop" for researchers new to geminiviruses, facilitating the establishment of a robust geminivirus research program.  
Extraction of geminivirus and associated satellite DNA from leaf
samples.- Mechanical sap transmission of geminiviruses.- Cloning of complete
geminivirus genomes by rolling circle amplification (RCA).- Biolistic
inoculation of geminiviruses.- Agroinoculation of plants with geminiviruses.-
Begomovirus transmission by the insect vector, the whitefly Bemisia tabaci.-
Mastrevirus transmission by the insect vectors, leafhoppers.- Transmission of
grabloviruses by insect vectors.- Sequence Demarcation Tool (SDT), a free
user-friendly computer program using pairwise genetic identity calculations
to classify nucleotide or amino acid sequences.- Phylogenetic analysis of
geminiviruses.- Recombination analysis of geminiviruses using Recombination
Detection Program (RDP).- Detection of tomato leaf curl New Delhi virus-ES by
real-time quantitative PCR.- Detection of geminiviruses using loop-mediated
isothermal amplification (LAMP).- Tissue printing hybridization using
digoxigen-labeled DNA probes for the detection of geminiviruses and
associated DNA satellites.- High throughput sequencing (HTS)-based diagnosis
of geminiviruses.- CRISPR-Cas-based diagnosis of geminiviruses.- Full-length
sequencing of circular DNA viruses using CIDER-Seq.- Subcellular localization
of geminivirus proteins by laser scanning confocal microscopy.-
Identification of interaction between geminivirus and plant proteins by
bimolecular fluorescence complementation (BiFC).- Exploring the effect of
tomato yellow leaf curl virus (TYLCV) infection on whitefly probing and
feeding behaviour by Electrical Penetration Graph (EPG) technique.