Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms.
Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement.
Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Genome engineering as a tool for enhancing crop traits: Lessons from
CRISPR/Cas9.- Genome editing for food security in vegetable
crops.- CRISPR/Cas9-mediated targeted mutagenesis in medicinal
plants.- Genome Editing: a review of the challenges and approaches.- Recent
advances and application of CRISPR base editors for improvement of various
traits in crops.- New Cas endonuclease variants broadening the scope of the
CRISPR toolbox.- Multiplexed genome editing in plants using CRISPR/Cas-based
endonuclease systems.- Transgene free genome editing in plants.- Genome
editing by Ribonucleoprotein based delivery of Cas9 system in plants.- Virus
mediated delivery of CRISPR/Cas9 system in plants.- Characterization of gene
edited crops via metabolomics.- Genome editing in plants for improvement of
resistance in plants against bacterial pathogen.- Improvement of resistance
in plants against Insect-pests using genome editing tools.- Applications of
gene drive for weeds and pest management using CRISPR/Cas9 system in
plants.- Recent trends in targeting genome editing of tomato for abiotic
stress tolerance.- Biosafety issue related to genome editing in plants using
CRISPR-Cas9.- Regulatory constraints and differences of Genome edited crops
around the globe.
Dr. Shabir Hussain Wani received his PhD in Genetics and Plant Breeding on Development of Transgenic Rice for Drought and Salt Tolerance from Punjab Agricultural University. He has published more than 130 peer-reviewed papers and edited 20 books on plant stress physiology, including 10 with Springer. He is on Editorial Boards of Plant Cell Tissue and Organ Culture, Frontiers in Plant Science and Frontiers in Genetics. He is currently an Assistant Professor (Senior Scale), Genetics and Plant Breeding, at the Mountain Research Centre for Field Crops of the Sher-e-Kashmir University of Agricultural, Sciences and Technology of Kashmir in India. Dr. Goetz Hensel is a the Head of the Centre for Plant Genome Engineering at Heinrich-Heine-University Düsseldorf, Germany. Before, he was a Senior Researcher at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany. He is on the Editorial Boards of BMC Plant Biology, Frontiers in Plant Science, Plant Cell Tissue and Organ Culture, and the Journal of Applied Genetics. He is member of the Management Board of the Society for Plant Biotechnology, Germany. He serves in the EFB Plant, Agriculture and Food Division Board and the German Joint Synthetic Biology Working Group. He was involved in the improvement of methods of Agrobacterium-mediated gene transfer to cereals and in a new principle of RNA-mediated downregulation of genes called host-induced gene silencing (HIGS). He recently focused his research on designer endonuclease-mediated gene targeting (TALEN, CRISPR/Cas9), molecular farming and genes involved in the spike architecture and domestication of barley. He has published over 100 articles in this field.
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