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E-grāmata: Genotype Phenotype Coupling: Methods and Protocols

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  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2681
  • Izdošanas datums: 05-Jul-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071632796
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Genotype Phenotype Coupling: Methods and ProtocolsPalielināt
  • Formāts: EPUB+DRM
  • Sērija : Methods in Molecular Biology 2681
  • Izdošanas datums: 05-Jul-2023
  • Izdevniecība: Springer-Verlag New York Inc.
  • Valoda: eng
  • ISBN-13: 9781071632796
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This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Genotype Phenotype Coupling: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics.
Quantitative Determination of Staphylococcus aureus Using Aptamer-Based
Recognition and DNA Amplification Machinery.- Construction of Synthetic VHH
Libraries in Ribosome Display Format.- Isolation of Adhirons Specific for
Plant Protoplast Membrane Biomarkers is Simplified by Phagemid Design.-
Facile One-Step Generation of Camelid VHH and Avian scFv Libraries for Phage
Display by Golden Gate Cloning.- Identification of New Antibodies Targeting
Tumor Cell Surface Antigens by Phage Display.- Phage Display of Bovine
Ultralong CDRH3.- Bacterial Cell Display for Selection of Affibody
Molecules.- Isolation of Antigen-Specific Unconventional Bovine Ultra-Long
CDR3H Antibodies Using Cattle Immunization in Combination with Yeast Surface
Display.- Selection of High-Affinity Heterodimeric Antigen-Binding Fc
Fragments from a Large Yeast Display Library.- A Two-Step Golden Gate Cloning
Procedure for the Generation of Natively Paired YSD Fab Libraries.-
Single-Cell B-Cell Sequencing to Generate Natively-Paired scFab Yeast Surface
Display Libraries.- One-Pot Droplet RT-OE-PCR for the Generation of Natively
Paired Antibody Immune Libraries.- Affinity Maturation of the Natural Ligand
(B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast Surface Display.-
Accessing Transient Binding Pockets by Protein Engineering and Yeast Surface
Display Screening.- Tyrosine Phosphorylation Screening on the Yeast Surface
by Magnetic Bead Selection and FACS.- Bulk Reformatting of Antibody Fragments
Displayed on the Surface of Yeast Cells to Final IgG Format for Mammalian
Production.- Antibody Secreting Cell Isolation from Different Species for
Microfluidic Antibody Hit Discovery.- Efficient Microfluidics Downstream
Processes for Rapid Antibody Hit Confirmation.- Cell Line Development Using
Targeted Gene Integration into MAR-Rich Landing Pads for Stable Expression of
Transgenes.- Generation of Human 293-F Suspension NGFR Knockout Cells Using
CRISPR/Cas9 Coupled to Fluorescent Protein Expression.- Antibody Display
Technology (ADbody) to Present Challenging and Unstable Target Proteins on
Antibodies.- SUMO: In Silico Sequence Assessment Using Multiple Optimization
Parameters.- Streamlined Data Analysis Pipeline for Deep Sequence-Coupled
Biopanning Identification of Pathogen-Specific Antibody Responses in Serum.
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