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Germline Development in the Zebrafish: Methods and Protocols 2021 ed. [Mīkstie vāki]

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  • Formāts: Paperback / softback, 383 pages, height x width: 254x178 mm, weight: 758 g, 76 Illustrations, color; 11 Illustrations, black and white; XIV, 383 p. 87 illus., 76 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2218
  • Izdošanas datums: 20-Feb-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071609726
  • ISBN-13: 9781071609729
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  • Mīkstie vāki
  • Cena: 118,31 €*
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  • Standarta cena: 139,19 €
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  • Formāts: Paperback / softback, 383 pages, height x width: 254x178 mm, weight: 758 g, 76 Illustrations, color; 11 Illustrations, black and white; XIV, 383 p. 87 illus., 76 illus. in color., 1 Paperback / softback
  • Sērija : Methods in Molecular Biology 2218
  • Izdošanas datums: 20-Feb-2022
  • Izdevniecība: Springer-Verlag New York Inc.
  • ISBN-10: 1071609726
  • ISBN-13: 9781071609729
Citas grāmatas par šo tēmu:
This volume details a wide range of methods, ranging from beginner through advanced, used to





further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.





 





Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Separation of Oocyte and Follicle Layer for Gene Expression Analysis in
Zebrafish.- The Xenopus Oocyte as an Expression System for Functional
Analyses of Fish Aquaporins.- Computer-Assisted Sperm Analysis to Test
Environmental Toxicants.- Cryopreservation and Transplantation of
Spermatogonial Stem Cells.- Masculinization of Zebrafish through Partial
Depletion of Primordial Germ Cells by Injecting Diluted Morpholino
Oligonucleotides into Embryos.- Chemical Genetics: Manipulating the Germline
with Small Molecules.- In vitro Induction of Teleost PGCs.- Applying Rho
Pathway Inhibitors to Investigate Germ Plasm Localization.- Cryopreservation
of Pooled Sperm Samples.- Quantifying Tissue Tension in the Granulosa Layer
after Laser Surgery.- In-vivo and Ex-vivo CT Imaging of Zebrafish
Gonads.- Live and Time-lapse Imaging of Early Oogenesis and Meiotic
Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries.- Detection of
the Polar Body after Fertilization.- Labelling the Micropylar Cell in
Zebrafish Whole-mount and Cryo-sectioned Follicles.- Visualisation of
Transcriptional Activity in Early Zebrafish Primordial Germ
Cells.- Experimental Methodologies to Visualise Early Cell Biology in
Zebrafish Embryos.- Observation of Medaka Larval Gonads by
Immunohistochemistry and Confocal Laser Microscopy.- Methods for
Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish
Embryo.- Glyoxal Fixation as an Alternative for Zebrafish Embryo
Immunostaining.- Histological Analysis of Gonads in Zebrafish.- Manipulating
and Visualizing the Germline with Transgenic Lines.-  Liquid Chromatography
and Tandem Mass Spectrometry in Label Free Protein Quantification of
Zebrafish (Danio rerio) Eggs.- In vivo Protein Lifetime Measurements across
Multiple Organs in the Zebrafish.- In vivo Imaging of Protein Interactions in
the Germ Plasm with Bimolecular Fluorescent Complementation.- The Zygotic
Genome Activation: Critical Prelude to the Most Important Time of Your
Life.- Identification of RNA-binding Protein Landscapes Across Zebrafish
Embryonic Transcriptome via iCLIP Approach.- Tethered Function Assay to Study
RNA Regulatory Proteins in Zebrafish Embryos.- -Massively Parallel Analysis
of Regulatory RNA Sequences.- Exploring Translational Control of Maternal
mRNAs in Zebrafish.