This book comprehensively reviews cell-free therapy approaches, focusing on the therapeutic potential of mesenchymal stem cell-derived extracellular vesicles (EVs) and secretomes across various reparative and regenerative medicine fields. The initial chapters primarily provide foundational insights into the application of EVs in dentistry, cardiovascular pathologies, and kidney injury. Each chapter discusses the role of mesenchymal stem cell exosomes in targeted therapies for conditions like neurodegenerative disorders, ophthalmological diseases, and diabetes complications, examining the applications of EV-based techniques in personalized medical strategies. The subsequent chapters discuss the advanced diagnostic and therapeutic applications, including using EVs as biomarkers in liquid biopsies and novel tissue engineering and cell-free scaffolding techniques for organ rejuvenation and tissue regeneration. Additionally, this book examines the challenges and progress in transitioning these therapies from laboratory research to clinical applications, covering critical aspects of formulation, Good Manufacturing Practice (GMP) production, regulatory approval, and market access. With insights into emerging technologies like 3D cell scaffolding and theragnostic applications in cancer and neurodegenerative diseases, this book emphasizes the future of a cell-free approach in regenerative medicine. This book is a useful resource for researchers and students in regenerative medicine.
Key Features:
- Provides insights into EV-based therapies and their role in regenerative medicine across multiple medical fields
- Discusses the potential of mesenchymal stem cell-derived exosomes in treating neurodegenerative, ophthalmological, and cardiovascular conditions
- Explores cell-free scaffolding for targeted tissue and organ regeneration
- Reviews the current regulatory, production, and clinical challenges in bringing cell-free therapies to market
- Explores cutting-edge theragnostic applications of EVs in oncology and neurological disorders
This book highlights the therapeutic potential of cell-free and cell-based therapies, including extracellular vesicles (EVs) and mesenchymal stem cell-derived exosomes across regenerative medicine. It explores their applications in liquid biopsies and tissue engineering. It is a useful resource for researchers of regenerative medicine.
About the Editor. List of Contributors.
Chapter 1 Extracellular Vesicles
as in Dentistry from Laboratory to Clinic.
Chapter 2 Extracellular
VesiclesBased Cell-Free Therapies in Cardiovascular Diseases.
Chapter 3
Regenerative Therapy with Extracellular Vesicles in Acute and Chronic Kidney
Injury.
Chapter 4 Isolation and Characterization of IPSC EVs Preconditioned
with Severe Critically Ill COVID-19 Patients Serum.
Chapter 5 Therapeutic
Efficacy of Mesenchymal Stem Cells and Their Derived Exosomes in Different
Neurodegenerative Disorders: Current Status and Future Perspectives.
Chapter
6 Applications and Implications of Mesenchymal Stem Cell-Derived Exosomes in
Ophthalmology.
Chapter 7 Future Management of Type 2 Diabetes and Its
Complications by Mesenchymal Stem CellDerived Exosomes (MSC-Exos).
Chapter 8
Liquid Biopsy Biomarkers for Personalized Management in Heart Failure.
Chapter 9 Prospective Implications of Autologous Platelet-Rich Plasma in
Tissue Engineering and Organ Rejuvenation.
Chapter 10 Cell-Free and
Cell-Based Therapy in Regenerative Medicine.
Chapter 11 Mesenchymal Cell-Free
and Cell-Based Therapy in Regenerative Medicine: A Crucial Exploration of
Formulation, GMP Production, Regulatory Approval, and Market Access.
Chapter
12 Unveiling Mesenchymal Stem Cells Secretome Potentials in Regenerative
Medicine and Disease Therapeutics.
Chapter 13 Engineering Therapeutic
Mesenchymal Stem Cells Secretome for Bone Regeneration.
Chapter 14 Cell-Free
Scaffolding: A Tissue-Engineering Approach with the Potential to
Revolutionize the Regeneration of Chondral Tissue.
Chapter 15 The Secretome
of Mesenchymal Stromal Cells in Treating Intracerebral Hemorrhage: Our
Experience.
Chapter 16 Stem Cell Secretome as Theranostics for Traumatic
Brain Injury.
Chapter 17 Cell-Free and Cell-Based Therapies in Regenerative
Medicine.
Chapter 18 Mesenchymal Stem Cell Secretomes for Cell-Free Therapy
of the Broken Heart.
Chapter 19 From 2D to 3D and Cellular to Cell-Free:
Evolutions in Strategies for Regenerative Medicine.
Chapter 20 Cell-Free and
Cell-Based Approaches in Neurological Surgery.
Chapter 21 A Clinical Overview
of Cell-Free and Cell-Based Therapies in Regenerative Medicine.
Chapter 22
Autologous versus Allogenic Mesenchymal Stem Cells in Heart Disease:
Revisiting Preclinical and Clinical Trials.
Chapter 23 Exosomes and
Encapsulated ExomiRs in Breast Cancer: Theragnostic Applications and Clinical
Implications.
Chapter 24 The Potential of Mesenchymal Stem CellDerived
Exosomes in Cell-Free Therapy for Myocardial Repair.
Chapter 25 Theragnostic
Applications of Exosomes in Alzheimers Disease. Index.
Dr. Khawaja Husnain Haider is a Professor of Cellular and Molecular Pharmacology (Stem Cell and Gene Therapy) and Chairman of the Basic Sciences Department (Medical Program) at Sulaiman AlRajhi University. Before his current assignment, he served in several prestigious institutions in various parts of the world. He has also served as Principal Investigator (PI) and Co-PI on multiple National Institute of Health (NIH) funded stem cell research projects. He has been on the editorial boards and invited reviewer panels for several respected international journals. His research focuses on using, either alone or in combination, DNA, miRNAs, and stem cells as drugs, a topic that has gained popularity in regenerative medicine. He has extensively published on cellular and sub-cellular preconditioning of stem cells and using their conditioned media for cell-free therapy. He has published nearly 350 book chapters, abstracts, and research papers in various books and leading research journals, including Circulation, Circulation Research, Cardiovascular Research, JCMM, the Journal of Biological Chemistry, Cell Cycle, Basic Research in Cardiology, and Antioxidant Redox Signaling. He has also given numerous presentations and edited ten books covering various facets of stem cells and their applications from drug to drug development.
