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E-grāmata: High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants

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  • Formāts: 280 pages
  • Izdošanas datums: 01-Jan-2011
  • Izdevniecība: Thieme Medical Publishers Inc
  • Valoda: eng
  • ISBN-13: 9781638532545
  • Formāts - EPUB+DRM
  • Cena: 149,01 €*
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  • Formāts: 280 pages
  • Izdošanas datums: 01-Jan-2011
  • Izdevniecība: Thieme Medical Publishers Inc
  • Valoda: eng
  • ISBN-13: 9781638532545

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With practical examples of how to establish the identity and quality of botanical products



High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance thin-layer chromatography (HPTLC) to establish the identity, purity, quality, and stability of raw materials, extracts, and finished botanical products.



The text provides a complete overview of the technique and common applications of HPTLC in herbal analysis. It will help the analyst answer questions such as:







Am I paying for a high-quality material, but getting a cheap adulterant? Is this raw material worth its price? Does this product comply with the claim on its label? Has the composition of this product changed after being on the shelf for more than a year?

Practical examples provided by renowned experts help the reader gain a firm understanding of HPTLC methodologies. More than 300 full-color illustrations aid comprehension of complex concepts and easy-to-reference text boxes provide summaries of key information.



This book is essential for analysts, quality assurance professionals, and regulators seeking a comprehensive text on how to use HPTLC to determine whether botanicals comply with current, good manufacturing practices. It will also benefit students in pharmacognosy, phytopharmacy, pharmaceutical biology, and analytical chemistry programs.

Recenzijas

A handy tool for analysts...A highlight of the book is the inclusion of modern instrumentation techniques...fair, updated, simple yet thorough [ discussions] of theoretical and practical aspects of modern TLC.--Doody's Book Reviews



"A comprehensive book...Well organized...Provides a very good understanding of HPTLC technique and serves as a good practical tool. This volume is therefore a must for institutions and persons involved in TLC studies of medicinal plants."-- Phytomedicine



"The text experly presents a basic but comprehensive introduction to TLC and further presents the technique's more modern modifications and applications. The information is presented in a clear and concise manner, including very useful definitions of validation parameters. The authors have presented information in a clear and concise manner with numerous practical examples and illustrations throughout the text. The appendices contain especially useful resources including invaluable lists for mobile phases and reagent discriptions, a template for a HPTLC standard operating procedure, and even an example of a validation protocol for identity...This book provides an exceptional introduction and overview to the many applications of HPTLC in medicinal plant analysis."--HerbalGram

Foreword xi
Preface xiii
Acknowledgments xv
Introduction
1(21)
Analysis of Herbal Drugs
1(5)
What Is Chromatography?
6(5)
What Is TLC?
11(8)
Modern TLC
19(3)
Theoretical Concepts
22(45)
General Chromatographic Principles
22(11)
Special Aspects of TLC
33(9)
The Stationary Phase
42(6)
The Mobile Phase
48(7)
The Chromatographic Result in TLC
55(6)
Densitometry
61(6)
Practical Aspects of Modern TLC
67(62)
Sample Preparation
68(5)
Selecting the Stationary Phase
73(19)
Sample Application
92(5)
Chromatogram Development
97(13)
Derivatization
110(4)
Visualization and Documentation of the Chromatogram
114(2)
Densitometry
116(5)
Reporting and Record Keeping
121(4)
TLC Software
125(1)
Standardization
125(2)
Practical Recommendations
127(2)
Typical Applications in Herbal Analysis
129(46)
Why HPTLC?
129(3)
HPTLC Analysis of Raw Material
132(28)
HPTLC Analysis of Herbal Drug Preparations and Products
160(15)
Method Development
175(18)
Theoretical Aspects
175(4)
Method Development in Practice
179(14)
Validation
193(22)
General Information
193(13)
Validation of Qualitative HPTLC Methods
206(4)
Validation of Quantitative HTPLC Methods
210(4)
The Final Word
214(1)
List of Abbreviations
215(3)
Glossary of TLC Terms
218(41)
Appendixes
Definitions
224(3)
Standard Operating Procedure for HPTLC of Herbal Raw Materials and Preparations
227(4)
Resources
231(2)
Common Mobile Phases for the Screening of Unknown Herbal Drugs
233(1)
Most Common Nonspecific Derivation Reagents for Analysis of Herbal Drugs
234(4)
Worksheet
238(2)
Validation of Method for Identification of Eleuthero by HPTLC Fingerprint
240(19)
Index 259